FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005284943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83106953
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1136018413.669354476273485No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA17201292.0697774829983238No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG8762611.0543774839152147No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG6882520.8281521282581494No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6017070.7240152337193736Clontech SMART CDS Primer II A (100% over 26bp)
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA5785210.6961162443291598No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3555880.42786793061706885No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG3021580.36357728095265385No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCG2284480.27488434090466535No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG2239060.2694190942122496No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG2158990.25978452127826174No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC2142260.2577714526484926No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG1972310.2373219001303056No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGC1826960.21983238875332126No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1708400.20556643437523214No Hit
GTGAAATCTGTCCCAGACCCGCTGCCAGTGAATCGGGCAGGGACCCCGGA1448970.1743500330231094No Hit
ATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCA1403550.16888478633069365No Hit
TATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGC1378800.16590669615814216No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1171550.1409689511778876No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT974410.117247710910542No Hit
CCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGG902160.10855409414420476No Hit
CTAAAATCTGTCCCAGATCCACTTCCATTGAACCTTGATGGGACCCCTGT874130.1051813318194929No Hit
GATGCATCGTAGATCAGGACCTTAGGGGCTTTCCCTGGTCTCTGCTGATA865000.10408274744472944No Hit
GGACCTTAGGGGCTTTCCCTGGTCTCTGCTGATACCAATTTAAAAAGTAG850890.10238493522918594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)