FastQCFastQC Report
Thu 12 May 2022
EGAF00005284947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77624786
Sequences flagged as poor quality0
Sequence length91
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT75868899.773796993140826No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA30469923.9252823189747668No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG13159521.6952729505753483No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG8979131.1567349119648458No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG6055110.7800485272835406No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG4252410.5478160029967748No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT4206180.5418604310226376No Hit
GGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACT4111870.5297109611355322No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA3534650.4553506917236461No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3158120.4068442778058029Clontech SMART CDS Primer II A (100% over 26bp)
GGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTGGG3039870.3916107414453935No Hit
GGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAGGTCACACTG2779630.35808536721763073No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2644210.34063990849520664No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1845010.2376831029202451No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC1703900.21950463090487618No Hit
CTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCG1479140.19054996170939525No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1471850.18961082868556958No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1456680.18765655598715597No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT1313920.16926552299931621No Hit
GTTGGTACCAGGCTAAGAAATTGCTGACACTCTGACTGGCCCTGCAGAAG1248320.16081461403320327No Hit
GGTTTGTGTTGGTACCAGGCTAAGAAATTGCTGACACTCTGACTGGCCCT1095080.14107349680809425No Hit
GTCGGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGCTAAGAAATTGCTG1009550.13005510894419728No Hit
GCCTGGCCAGGTTTGTGTTGGTACCAGGCTAAGAAATTGCTGACACTCTG1008630.12993659009894082No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG976170.12575493605869653No Hit
GGAGTCGGGGAGCCTGGCCAGGTTTGTGTTGGTACCAGGCTAAGAAATTG912650.11757198274272859No Hit
GTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCTTT881140.11351271229269476No Hit
GCTCGCTCCCCTGGAGACAAAGACAAGGTGACTGGAGACTGTGTCATGAC875110.11273589855693773No Hit
GCCCTGTTGGATGCATCATAGATGAGGAGTCGGGGAGCCTGGCCAGGTTT819520.10557452615714778No Hit
CTAAGAAATTGCTGACACTCTGACTGGCCCTGCAGAAGAGGGTGGCTCGC818580.10545343081525532No Hit
GTCTGTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCTTTGGCCC791060.1019081714441055No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)