FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005284994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325516460
Sequences flagged as poor quality0
Sequence length16
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCTCGGTGGTCGC43460531.3351254188497872Illumina Single End PCR Primer 1 (100% over 16bp)
TTCCCATCTAACGAAT7741390.23781869586564072No Hit
TGTTACTTGGGTTTGT7310920.22459447979988478No Hit
TGTGGTAACCAGGTGA7159670.21994801737521968No Hit
CAATTCGGACTCACGG7097200.21802891319228526No Hit
CTAGAGACTACCTAGT6702710.20591001757637695No Hit
TATGGGTACAGAGGCT6311430.19388973448531605No Hit
CGGACGTTGGGTGTCC6297420.19345934150303798No Hit
TGGGCCATGCCTACGG6026450.18513503126692887No Hit
TTTGCGGCTTCTGTAG5844000.17953009196524194No Hit
CTTGCTGACTGGCATT5819790.17878635077316826No Hit
TGTACGATGAAAGCTA5674980.17433772780645257No Hit
ACTTATCACTTACGAC5400330.16590036645151524No Hit
AGACTACTGCGGTTGC5380850.1653019328116311No Hit
GGTATTGACGACTCGC5358250.16460765148404477No Hit
TTCCTACCTTTTCCGC5305100.1629748615477079No Hit
CCGTTGTACTTTCTTG5294780.16265782688838532No Hit
TCAATGGACTTCGGGC5241050.1610072191126679No Hit
ACTGATTCTCTCCTAC5220380.16037222818164096No Hit
CTGTTGCTGACCGTGC5134140.1577228997882319No Hit
CTCTCTTTGTAACGAA4925660.1513183081433117No Hit
TGGACAACTGCCGTTG4845050.14884193567354473No Hit
GCATCAATGAGGACAC4835850.1485593078764742No Hit
GCATCAATGCCTGCGA4796130.1473390930830349No Hit
GTTGATGACAAGGCTG4778260.14679011930763808No Hit
TCTTTAGACCCCGAGT4729910.1453047873523815No Hit
CGGTCTACTCACAGTT4700960.14441543140399107No Hit
TTAAGGAGAGTTCTCC4673730.14357891456548771No Hit
CAATGCCCTGCTAATC4628660.14219434556396932No Hit
GCACAATTGACCATGT4613210.14171971518736717No Hit
AGGGAGTTGTTGTGAC4593960.1411283472424098No Hit
GCAGACGCTTGGGCAA4548300.13972565319738361No Hit
AGAGACCGAGCTTACA4383610.13466630842569374No Hit
TTCCGGACTGCCCTGA4372710.13433145592699058No Hit
GGTATACTGGCCCAAC4355750.1338104377271736No Hit
AGCTGATACTCATTGG4318910.13267869772238247No Hit
GTATGAAGAAGCCATT4250580.1305795719208792No Hit
GCAGACGCTTGCAAGG4204150.12915322315805475No Hit
GGCAACGCTATCCGTA4143900.12730231829136995No Hit
TCCGTTGACTGTCACA4138570.12713857849154542No Hit
TAGTACCGACTATCTT4021480.1235415253655683No Hit
TGTAACGTGACGCCAC4014440.1233252536599839No Hit
TGTTGTACTCGCCACA3956640.1215496138044755No Hit
AACTCCCTGTCTATCT3888390.11945294563599027No Hit
CGTAATCGAATAACCC3876190.11907815660074454No Hit
CGTGTACCTCTTGAAC3870230.11889506294090321No Hit
CTGGGACACCCTCAAA3845200.118126131010395No Hit
GGGCTTCGAGGCATTT3767530.1157400765540397No Hit
TGTGGTAACTACGTTG3744050.11501876126325532No Hit
GTATAGCTGCATTCAG3727710.11451678971932786No Hit
TAACAGAGAGGCCAAA3720130.11428392899087193No Hit
CTTACATTGAGGACCA3692560.11343696721204206No Hit
TGTCGCTTGTCTGTAG3659910.1124339457365689No Hit
CCTGATCACTTCCATT3648590.11208619066452123No Hit
GGGCTGACTCCTGTGG3583540.11008782781675618No Hit
GAGAGAAGAATCAGCT3521130.10817056685858528No Hit
AAGACCTACGTTCTCC3486460.1071054901494075No Hit
CTCTGACTGGGACCAA3457790.10622473591658008No Hit
CCGTACGGATTTCCGC3418080.10500482832726798No Hit
AAGTGGCGAAGGCATG3418050.10500390671488624No Hit
GCATATCGAAGCAAGC3390830.10416769708051016No Hit
CTTGCTGACTTAGAAG3350420.10292628520229054No Hit
AATTCGGTGACGTGCC3328370.10224890010170298No Hit
ATACAGGCTTGAAAGT3296780.1012784422637184No Hit
GCCTAAATGAGGACCA3292650.1011515669591639No Hit
CGCTTCAACGCGGTAA3280480.10077769953629995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)