FastQCFastQC Report
Fri 3 May 2024
EGAF00005285863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005285863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393787456
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACGCC14341025736.41818824213639No Hit
AGCCAATT11873839830.152915282298885No Hit
CTAGGTGA10904228127.690643604452447RNA PCR Primer, Index 38 (100% over 8bp)
TCGTTCAG151568033.8489806541729963No Hit
GATACGCA8557780.217319771607961No Hit
AGCCAATA7394230.18777210617902465No Hit
CTAGGTAA4293060.10901972458970353No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)