FastQCFastQC Report
Tue 10 May 2022
EGAF00005286101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103245
Sequences flagged as poor quality0
Sequence length35-150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG125891.1410883348666887No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG102830.9320685795086313No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG101980.9243640351871072No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT101780.9225512012291014No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA96070.8707947917280386No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT87940.7971030913351069No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC82260.745618606927745No Hit
GGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC80620.7307533684720982No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA79720.7225956156610726No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG76050.6893301125316679No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC75460.6839822523555511No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC72500.6571523097770667No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA66140.5995041899124854No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG59770.5417654283500039No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG57470.5209178378329382No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA53220.4823951162253171No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC51640.4680737279570721No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG48040.43544271671296947No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC47490.43045742332845377No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC46150.4183114358098156No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT44130.400001812833958No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC42180.3823266817434024No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG41710.378066521942089No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC39640.35930369047673No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT38610.34996759559300067No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT38560.34951438710349925No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG37820.34280690145887815No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC36750.33310823978354764No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG35970.32603818734732537No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA34700.31452669171398917No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGACGG33580.3043748215491573No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG33290.30174621231004894No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT32870.29793926099823703No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC32680.2962170687381316No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA31800.2882405993229065No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG30980.2808079800950831No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG30770.27890450443917714No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT30440.2759133284084678No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT30190.27364728596096066No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC30140.2731940774714592No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC30060.2724689438882569No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC29810.2702029014407498No Hit
CTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTC29670.2689339176701458No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA28980.2626796405150261No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC28320.2566972884536073No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG28040.25415932091239934No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG27990.25370611242289787No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC27650.2506242946942882No Hit
CCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGG27160.24618285149717425No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA27030.24500450942447055No Hit
CACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCT26780.2427384669769634No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT26430.23956600755045346No Hit
CTGCTGGAGCGGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGT26200.23748124849874686No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG26120.2367561149155446No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG25310.2294141373856215No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC24950.22615103626121127No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC24390.2210751011787953No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT24200.2193529089186899No Hit
CTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATA24200.2193529089186899No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGGGGG24110.2185371336375873No Hit
TTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCG24060.21808392514808586No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT23810.21581788270057875No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT23770.2154553159089776No Hit
CTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACA23630.2141863321383736No Hit
CCCCGTCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC23460.21264542327406877No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA23320.2113764395034648No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA23270.21092323101396335No Hit
GGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCT23150.20983553063915994No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG22990.20838526347275538No Hit
CCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTC22670.20548472913994625No Hit
GTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAA22370.2027654782029377No Hit
ACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAG21850.19805210991212288No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGG21460.19451708369401177No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC21300.19306681652760718No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAAT20580.18654061427878665No Hit
CCCTTTATTCCCCCACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTG20340.18436521352917984No Hit
CAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTT20130.18246173787327385No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGACGGGG20090.1820991710816727No Hit
GCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCT19990.18119275410266986No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG19900.18037697882156728No Hit
GGACAGGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC19880.18019569542576672No Hit
GGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGC19040.17258179280214275No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT19000.17221922601054163No Hit
CCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCT18770.17013446695883508No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG18720.16968125846933366No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC18470.16741521602182652No Hit
CGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACG18210.1650585318764191No Hit
CACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCG18190.16487724848061855No Hit
CAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCG17830.16161414735620827No Hit
GGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCG17700.16043580528350457No Hit
GGCTAGGGGACAGGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA17570.15925746321080086No Hit
CCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGA17350.15726334585699459No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGAC17210.15599436208639061No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA17110.15508794510738774No Hit
TCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCT16690.15128099379557577No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG16470.1492868764417695No Hit
GTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGA16440.14901495134806864No Hit
CTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAG16400.1486523845564675No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA16360.14828981776486636No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGACGGGGTGGGCAGCAGCTCGAAT16360.14828981776486636No Hit
AACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGA16210.1469301922963621No Hit
ACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTA15680.14212618230764698No Hit
GAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACG15560.14103848193284357No Hit
GGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCC15480.1403133483496413No Hit
GATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAAC15320.13886308118323673No Hit
GCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC15290.13859115608953587No Hit
CGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAAC14940.1354186966630259No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA14680.13306201251761848No Hit
CTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGC14660.13288072912181792No Hit
CTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGC14520.13161174535121392No Hit
CTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCT14470.1311585368617125No Hit
CTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTC14450.13097725346591194No Hit
GACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGA14310.12970826969530794No Hit
TCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCC14300.12961762799740764No Hit
GGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAG14050.12735158554990053No Hit
TGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGC13770.12481361800869253No Hit
CCCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCC13570.12300078405068683No Hit
CTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTC13430.12173180028008283No Hit
CTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTC13380.12127859179058141No Hit
GTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCA13300.12055345820737914No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC13290.12046281650947886No Hit
CCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCC13250.12010024971787772No Hit
GCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGGTGTGACCG13250.12010024971787772No Hit
CCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCC13050.118287415759872No Hit
CCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCT13040.11819677406197174No Hit
GGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGTCTTGGTGCGG12960.11747164047876944No Hit
AGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTA12910.11701843198926802No Hit
GTCTAGCTCAGCTCAGTCCAGCTCAGCCCAGCCTAGTCCAGCCCAGCCCA12880.11674650689556716No Hit
GCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT12730.1153868814270629No Hit
AGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCG12690.11502431463546174No Hit
AACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTG12540.11366468916695749No Hit
GCGTCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC12490.11321148067745607No Hit
GCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATG12450.11284891388585491No Hit
GAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGC12440.11275827218795462No Hit
GAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCG12430.11266763049005435No Hit
AGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGAT12300.11148928841735062No Hit
GTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGG12260.11112672162574949No Hit
GGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTC12190.11049222974044749No Hit
CACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCT11930.10813554559504009No Hit
CAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGC11780.1067759201265358No Hit
TTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTG11760.10659463673073524No Hit
GCTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCA11760.10659463673073524No Hit
CTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGT11760.10659463673073524No Hit
TCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACC11730.10632271163703438No Hit
GTCGTTGTCTCCCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCG11630.10541629465803154No Hit
GAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCC11600.10514436956433068No Hit
CTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATC11510.10432859428322812No Hit
GGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGGCT11490.10414731088742755No Hit
GGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGG11430.10360346070002584No Hit
GGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGGGGGCT11400.10333153560632498No Hit
CCCGACGCCCAGCGAGGATATCTGGAAGAAATTCGAGCTGCTGCCCACCC11350.10287832711682356No Hit
GCTGCCCACCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGC11290.10233447692942184No Hit
TCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCT11190.101428059950419No Hit
ACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTC11120.100793568065117No Hit
GGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGC11060.10024971787771528No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)