FastQCFastQC Report
Tue 10 May 2022
EGAF00005286102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103245
Sequences flagged as poor quality0
Sequence length35-150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG126171.1436263024078968No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA100480.9107677805020644No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG96310.8729701924776454No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT94460.8562014783660927No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG89310.8095210039474459No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA88080.7983720751057108No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT84340.7644720800910043No Hit
GGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC77880.7059175432474202No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC75080.6805378678353403No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC72030.6528921499757533No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG71650.6494477654555425No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC68830.623886806647662No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA63920.5793817329786222No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG55390.502064364669679No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC52050.4717900375709838No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG49440.44813255441900934No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA48640.44088121858698653No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC47420.4298229314431518No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC47280.42855394767254784No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG47110.42701303880824293No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT44360.40208657188566455No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC40590.3679146517772571No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG40090.3633825668822428No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC39520.35821599010192656No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG38650.3503301623846018No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC37880.3433507516462798No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT37150.336733907699559No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG34870.31606760057829403No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT34840.3157956754845932No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG33730.30573444701766156No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA33540.3040122547575561No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT33520.3038309713617556No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG31800.2882405993229065No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC31730.2876061074376045No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGACGG31000.2809892634908837No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA30720.27845129594967577No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT30590.277272953876972No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG30590.277272953876972No Hit
CTGCTGGAGCGGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGT29970.2716531686071544No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC29450.2669398003163395No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC29260.2652176080562341No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC28600.2592352559948153No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG28330.2567879301515076No Hit
GGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCT28040.25415932091239934No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA27990.25370611242289787No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA27940.2532529039333965No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT27620.2503523696005874No Hit
CTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTC27450.2488114607362825No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC27010.24482322602866996No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT26390.23920344075885228No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG26170.237209323405046No Hit
CCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGG25990.2355777728428409No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT25810.23394622228063577No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC25680.23276788020793207No Hit
CACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCT25430.23050183776042493No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT25110.2276013034276158No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG25090.22742002003181525No Hit
CTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATA24920.22587911116751042No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC24450.221618951366197No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG24330.2205312509913936No Hit
GTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAA24320.22044060929349327No Hit
CCCCGTCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC23740.21518339081527676No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGGGGG23500.2130079900656699No Hit
TTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCG23200.21028873912866136No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA23070.20911039705595766No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG22240.201587136130234No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC22160.20086200254703174No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG22090.2002275106617297No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC21670.19642055934991776No Hit
CCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTC21340.19342938331920834No Hit
ACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAG21270.19279489143390632No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA20560.1863593308829861No Hit
CCCTTTATTCCCCCACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTG19840.17983312863416556No Hit
GGACAGGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC19780.17928927844676384No Hit
GCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCT19770.17919863674886358No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGG19520.17693259430135647No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGACGGGG19110.17321628468744477No Hit
GGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGC18950.1717660175210402No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG18910.17140345072943908No Hit
GGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCG18660.16913740828193194No Hit
CCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGA18600.16859355809453022No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT18230.16523981527221968No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC18160.1646053233869177No Hit
CAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTT18140.16442403999111713No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT18140.16442403999111713No Hit
CGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACG18120.16424275659531656No Hit
GATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAAC18020.1633363396163137No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA17950.1627018477310117No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA17770.16107029716880655No Hit
CACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCG17510.15871361302339917No Hit
GCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC17430.1579884794401969No Hit
CTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAG17360.15735398755489488No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA17360.15735398755489488No Hit
GTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGA16960.15372831963888348No Hit
GGCTAGGGGACAGGGACGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA16870.1529125443577809No Hit
CCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCT16760.1519154856808778No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGAC16360.14828981776486636No Hit
ACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTA16180.14665826720266123No Hit
CAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCG15270.1384098726937353No Hit
GAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACG15260.138319230995835No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGACGGGGTGGGCAGCAGCTCGAAT15210.1378660225063336No Hit
TCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCT15170.13750345571473244No Hit
CGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAAC15160.13741281401683217No Hit
GGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCC15010.13605318854832787No Hit
CTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGC14470.1311585368617125No Hit
CCCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCC14140.12816736083100308No Hit
GGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTC14130.1280767191331028No Hit
AACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGA14090.12771415234150166No Hit
CTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTC14060.12744222724780083No Hit
CTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGC13880.12581067668559567No Hit
CTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCT13770.12481361800869253No Hit
GACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGA13560.12291014235278656No Hit
CCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCC13490.12227565046748455No Hit
TCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTA13450.12191308367588342No Hit
CTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTC13410.12155051688428227No Hit
GCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGGTGTGACCG13270.12028153311367829No Hit
GTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCA13050.118287415759872No Hit
GTCTAGCTCAGCTCAGTCCAGCTCAGCCCAGCCTAGTCCAGCCCAGCCCA13020.11801549066617115No Hit
TCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCC12970.11756228217666972No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG12900.11692779029136774No Hit
CTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACA12550.11375533086485776No Hit
TGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGC12550.11375533086485776No Hit
GCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATG12500.11330212237535633No Hit
CCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCC12380.11221442200055293No Hit
GTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGG12170.11031094634464693No Hit
CCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCT12090.10958581276144465No Hit
GCTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCA12080.10949517106354435No Hit
GCGTCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC12000.10877003748034207No Hit
GCTGCCCACCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGC11870.10759169540763838No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAAT11760.10659463673073524No Hit
GAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGC11760.10659463673073524No Hit
GGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGTCGGGGGCT11620.10532565296013124No Hit
AACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTG11600.10514436956433068No Hit
GGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGC11590.10505372786643039No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC11460.1038753857937267No Hit
AGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTA11380.10315025221052442No Hit
AGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCG11360.10296896881472382No Hit
CACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCT11260.10206255183572098No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)