FastQCFastQC Report
Tue 10 May 2022
EGAF00005286142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497744
Sequences flagged as poor quality0
Sequence length35-150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA114750.7661522930487453No Hit
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT107790.719682402333109No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG104730.6992516745184757No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG93610.6250066767084361No Hit
CCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGG93280.6228033629245051No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG92150.6152586823916504No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG88230.5890859853219241No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGG82790.5527646914292429No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA82690.5520970205856275No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT80130.5350046469890716No Hit
CTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT79910.5335357711331176No Hit
GCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCGA79580.5313324573491865No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT79340.5297300473245093No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC74600.49808244933713636No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC73440.49033746755119706No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC71520.4775181873537801No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG70250.4690387676398637No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA63450.4236371502740121No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC60390.4032064224593789No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA56200.37523101411189097No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC55200.3685543056757363No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG54700.365215951457659No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAG53190.3551341217190655No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG52770.3523299041758805No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG52720.3519960687540728No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG51140.34144686942494845No Hit
AGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGC50390.33643933809783244No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA50200.3351707634949631No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC48990.32709194628721594No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG46100.30779625890672907No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG45520.30392376801375937No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC45090.30105278338621283No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT44040.2940422395282505No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA43870.2929071990941042No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC42450.2834262731147646No Hit
TTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC42220.28189063017444904No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG41860.27948701513743335No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT40900.2730773750387249No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA40130.26793630954288583No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCG40090.2676692412054396No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC39870.26620036534948566No Hit
GCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCA39600.2643976540717239No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC37130.2479061842344219No Hit
TAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACAC36830.2459031717035755No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT36740.2453022679443216No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC36520.24383339208836757No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA35970.24016120244848252No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC35910.23976059994231325No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT35810.23909292909869775No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG33360.2227349934301189No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT33040.2205984467305494No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG32540.2172600925124721No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG32240.2152570799816257No Hit
GTTCTGGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGA31930.21318730036641778No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG31710.21171842451046374No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG31390.20958187781089424No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT30880.20617675650845538No Hit
CCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAG30210.20170336185623178No Hit
GTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGC30150.2013027593500625No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG29780.19883237722868527No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC29680.1981647063850698No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT29580.19749703554145437No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC29570.1974302684570928No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT29320.19576109134805414No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC29230.19516018758880022No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT29200.1949598863357156No Hit
TACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT28980.19349101047976155No Hit
GGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTG28560.1906867929365766No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT28150.1879493424777532No Hit
CTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTC27800.18561249452509906No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA27510.18367624907861424No Hit
CTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAA27480.1834759478255296No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC27090.18087203153542927No Hit
CCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGG27060.18067173028234465No Hit
GGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTC27000.18027112777617538No Hit
CTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATA26580.1774669102329904No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC26440.17653217105192878No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA25650.17125757138736658No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG25600.17092373596555888No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAAT25070.1673850804943969No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC24880.16611650589152752No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGC24660.1646476300355735No Hit
GCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC24270.1620437137454732No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC24250.16191017957675008No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT24170.16137604290185772No Hit
GTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAA23700.15823798993686503No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG23670.1580376886837804No Hit
GGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGGCT23560.1573032507558034No Hit
TCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTA23060.15396489653772608No Hit
CACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCT22920.15303015735666442No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA22920.15303015735666442No Hit
ATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC21980.14675405142667908No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC21960.14662051725795597No Hit
ACTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTAC21730.1450848743176404No Hit
CCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTC21670.14468427181147112No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC21500.14354923137732484No Hit
GAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG21360.14261449219626318No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT21250.14188005426828618No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT21210.14161298593083999No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG20700.13820786462840112No Hit
CTGCTGGAGCGGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGT20660.13794079629095493No Hit
CCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGC20220.13500304457904688No Hit
GATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAAC20200.1348695104103238No Hit
GAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG20170.13466920915723915No Hit
ACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAG20110.13426860665106988No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCA20100.13420183956670834No Hit
GGGAAAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGT19960.13326710038564668No Hit
GCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCT19670.13133085493916183No Hit
CCCTTTATTCCCCCACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTG19660.1312640878548003No Hit
TACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAA19610.13093025243299256No Hit
CCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA19420.12966167783012317No Hit
TTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCG19400.12952814366140009No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATC19340.1291275411552308No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG19120.12765866529927677No Hit
CCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT19040.1271245286243844No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCG18970.12665715903385358No Hit
GCCTTCTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG18930.12639009069640741No Hit
GTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGAG18680.12472091358736873No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA18340.12245083271907614No Hit
GGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGC18330.1223840656347146No Hit
GGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATT18320.12231729855035306No Hit
CGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACG18200.12151609353801451No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA18190.12144932645365297No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA17920.1196466151758912No Hit
GCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT17800.11884541016355264No Hit
GGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCG17780.11871187599482955No Hit
GGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG17660.11791067098249101No Hit
CTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATC17510.1169091647170678No Hit
GCTGCCCACCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGC17370.11597442553600615No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC17300.11550705594547533No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC17110.11423848134260596No Hit
CCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGA17060.11390464592079821No Hit
CTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAG16590.11076659295580554No Hit
GGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT16480.11003215502782852No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCA16470.10996538794346697No Hit
GAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGC16370.1092977170998515No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA16250.10849651208751296No Hit
CTTCCAGATATCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCT16120.10762853999081284No Hit
CAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCG16020.10696086914719738No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC16010.10689410206283582No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGA15810.10555876037560491No Hit
AACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGA15700.1048243224476279No Hit
CAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTT15520.10362251492912007No Hit
GTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGA15400.10282130991678151No Hit
CTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTACC15340.10242070741061224No Hit
CTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTC15290.10208687198880449No Hit
GGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCC15240.10175303656699676No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG15200.10148596822955058No Hit
CCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCT15120.1009518315546582No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG15060.10055122904848893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)