FastQCFastQC Report
Wed 11 May 2022
EGAF00005286211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086204
Sequences flagged as poor quality0
Sequence length35-150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGA604682.8984701400246573No Hit
GCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAA583952.7991030599116864No Hit
GCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACG440702.112449213979074No Hit
CCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAG363721.7434536603323547No Hit
GCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTT338371.6219410949264788No Hit
ATCCAGGACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTC323101.5487459519778506No Hit
CCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGC278521.3350563990865707No Hit
CTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACA278451.3347208614306174No Hit
CTCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTC275881.3224018360620533No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC266871.279213346345803No Hit
GTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGCTG248961.1933636403726577No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT243221.1658495525845027No Hit
CCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCG205630.9856658313376833No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCG187810.9002475309221917No Hit
GTGTGCACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAG170630.8178970033611286No Hit
TCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCC161670.7749481833991306No Hit
GAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTC153650.7365051548170745No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG128550.6161909381824596No Hit
GAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACC124150.5950999998082642No Hit
GAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCA119200.5713726941372944No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC115810.5551230847989937No Hit
ACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGG115440.5533495286175273No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT112660.5400238902811039No Hit
GGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCG112330.5384420699030392No Hit
ATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTC109360.5242056865004573No Hit
TCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGA102570.49165853387300573No Hit
GTGTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGAC102340.4905560530034455No Hit
CTACCAGGCTGCGCGCTGTGTTCGGGGAGACAACGACGGCGGTGGCGGGA101470.4863857992794569No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA98750.47334776464813605No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG90450.43356258544226745No Hit
GCTCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGT85050.40767825198302754No Hit
GATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCT84910.40700717667112135No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC84130.40326832850478667No Hit
GCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGG82960.3976600562552847No Hit
GGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCT82670.39626997168062184No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT82340.3946881513025572No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT82140.3937294722855483No Hit
GGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGAC80610.3863955778054303No Hit
AGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGT80190.3843823518697117No Hit
CTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACG78750.3774798629472477No Hit
CTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGC73790.3537046233254274No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG73430.35197900109481145No Hit
GCCGAGAAGCCGCTCCACATACAGTCCTGGATGATGATGTTTTTGATGAA73110.3504451146675972No Hit
GGACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGA69140.3314153361799709No Hit
GACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCAT68310.32743681825938403No Hit
TGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAA68050.3261905355372725No Hit
TCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCC65460.31377564226700744No Hit
TCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCTGTGTTCGGGGAGAC65420.3135839064636057No Hit
CAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAG64550.309413652739617No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG62170.2980053724372113No Hit
GCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCTGGATGATGATG61990.2971425613219033No Hit
GACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGAT61180.2932599113030174No Hit
GGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGA59830.28678882793820737No Hit
CTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAG58490.2803656785242479No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC58150.2787359241953328No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGTTTCACCAACAG57240.27437393466794235No Hit
GCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGT57200.2741821988645406No Hit
CCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGAC55630.2666565685810208No Hit
TCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCT55040.26382846548084465No Hit
GTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCT54850.2629177204146862No Hit
CTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGA53710.25745325001773556No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA53460.25625490124647443No Hit
GGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGG52710.2526598549326911No Hit
GTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGC52510.25170117591568225No Hit
GAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGA51860.24858546911040338No Hit
GACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGG51420.24647637527298386No Hit
GAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACA49740.23842347153010926No Hit
GCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGG49610.23780033016905347No Hit
CTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTC48630.23310280298570993No Hit
ACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAG48290.23147304865679483No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA47670.22850114370406727No Hit
CCGCCAAGCTCGTCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCTGT47570.22802180419556284No Hit
GTGCAGCCATATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCA47090.22572097455474155No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA46860.22461849368518133No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG42780.2050614417382001No Hit
GTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGC42380.20314408370418233No Hit
CCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCA41210.19753581145468038No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT40120.19231101081198196No Hit
GTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAA40000.19173580340177662No Hit
GACGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAG39900.1912564638932722No Hit
GTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCA39800.19077712438476774No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC39690.19024985092541286No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT39590.1897705114169084No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT39100.18742174782523666No Hit
CAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGATGG38910.18651100275907823No Hit
CATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGT38080.18253248483849135No Hit
GCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACG37160.17812256136025048No Hit
CACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCG36780.1763010712279336No Hit
CCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATG36700.17591759962113004No Hit
GCCATATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCAGCAGC36440.17467131689901852No Hit
CCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTT35990.1725142891107485No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA35740.1713159403394874No Hit
GGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCA35640.17083660083098295No Hit
GAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAG34590.16580353599168635No Hit
TCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCC34520.16546799833573322No Hit
ATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGCTGGC34370.16474898907297655No Hit
CAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAG34130.1635985742525659No Hit
CGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCG34030.16311923474406145No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTTCGCACCAAG33820.16211262177620214No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG33260.15942832052857728No Hit
GCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAA32260.15463492544353286No Hit
GCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAG31450.15075227542464686No Hit
CAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGC31310.15008120011274065No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG31070.14893078529233No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT30270.14509606922429447No Hit
CACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGG30060.14408945625643513No Hit
CTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGG29630.14202829636986603No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG29610.14193242846816514No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC29140.13967953277819428No Hit
CCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAG28770.13790597659672782No Hit
CTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCT28660.13737870313737297No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTTCGCACCAAGACCCCTTTAACT28260.13546134510335517No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC28060.13450266608634628No Hit
GAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGC27740.1329687796591321No Hit
CACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCC27530.13196216669127278No Hit
TATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCAGCAGCAGAG26670.12783984691813458No Hit
TGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAACTAA26010.12467620616200525No Hit
TGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGAC25760.12347785739074416No Hit
ACGTTAGTTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG25270.12112909379907238No Hit
CTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGT25100.12031421663461483No Hit
GCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAG24570.11777371723954129No Hit
GACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGCTTCT24390.1169109061242333No Hit
CCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCC24330.11662330241913063No Hit
CCACACACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCG24300.11647950056657931No Hit
CCTACCAGGCTGCGCGCTGTGTTCGGGGAGACAACGACGGCGGTGGCGGG23840.11427453882745886No Hit
GAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCTGGAT23770.11393900117150577No Hit
TCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGAC23600.1131241240070482No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA23530.11278858635109511No Hit
GTGCACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGA23200.11120676597303045No Hit
CGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAG22940.1099604832509189No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG22930.10991254930006844No Hit
CACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTG22570.10818692706945246No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA22570.10818692706945246No Hit
CGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTA22420.10746791780669579No Hit
CCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGC21810.1045439468048187No Hit
GAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCAT21770.10435221100141692No Hit
AAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAG21750.10425634309971603No Hit
TGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGC21360.10238691901654873No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG21220.10171584370464251No Hit
AGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCTGGATGATGAT21010.10070923073678317No Hit
AGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCA20990.10061336283508228No Hit
GGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTT20930.10032575912997962No Hit
CTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTG20910.10022989122827873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)