FastQCFastQC Report
Wed 11 May 2022
EGAF00005286212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086204
Sequences flagged as poor quality0
Sequence length35-150
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAA622492.9838405064892983No Hit
GTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGA622012.981539676848477No Hit
GCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACG439112.1048277157938533No Hit
CCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAG329591.5798550860797889No Hit
GCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTT329481.579327812620434No Hit
ATCCAGGACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTC312831.4995177844544445No Hit
GTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGCTG251811.2070248163650343No Hit
CCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGC245041.1745735316392836No Hit
CTCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTC243331.1663768260438576No Hit
CTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACA237341.1376643894844416No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT231891.1115403862709494No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC215331.0321617636626141No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCG191220.916593008162193No Hit
CCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCG190500.9131417637009611No Hit
GTGTGCACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAG170290.8162672490322135No Hit
TCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCC146930.704293539845576No Hit
GAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTC129760.6219909462353633No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG128380.615376061018002No Hit
GAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCA113130.5422767859710748No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC113020.5417495125117199No Hit
GAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACC110200.5282321383718946No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA109420.5244932902055599No Hit
GGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCG108600.5205627062358236No Hit
ACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGG102380.4907477888068472No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT97140.4656303985612145No Hit
ATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTC97060.46524692695441094No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG93780.44952459107546533No Hit
TCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGA93640.44885351576355903No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC91030.43634275459159316No Hit
GTGTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGAC86800.41606669338185526No Hit
GATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCT85030.40758238408132663No Hit
CTACCAGGCTGCGCGCTGTGTTCGGGGAGACAACGACGGCGGTGGCGGGA84670.4058567618507107No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT84560.40532948839135574No Hit
GCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGG83730.4013509704707689No Hit
GGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCT78690.37719225924214506No Hit
GCTCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGT77740.3726385339113529No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT75640.3625724042327596No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG75220.3605591782970409No Hit
CTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGC73920.3543277646864832No Hit
AGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGT72970.349774039355691No Hit
CTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCTCTGAGACG71660.3434946917942828No Hit
GGACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGA69520.3332368263122878No Hit
GCCGAGAAGCCGCTCCACATACAGTCCTGGATGATGATGTTTTTGATGAA69180.3316070719833727No Hit
GGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGAC68770.32964177999850447No Hit
TGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAA66750.3199591219267147No Hit
CAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAG66450.3185211034012014No Hit
GACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCAT64800.31061200151087814No Hit
GCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCTGGATGATGATG63770.3056748045732824No Hit
TCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCTGTGTTCGGGGAGAC62220.2982450421914635No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG62100.2976698347812582No Hit
CTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGA60860.2917260248758031No Hit
GCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGT58110.27854418839193096No Hit
TCCACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCC57760.27686650011216546No Hit
GTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTTCT57270.27451773652049366No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC55720.26708797413867486No Hit
GAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGA55060.26392433338254556No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA54720.2622945790536304No Hit
GTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGC54540.2614317679383224No Hit
GACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGAT54450.2610003623806684No Hit
CTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAG54090.2592747401500525No Hit
GGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGA54070.25917887224835157No Hit
GGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGG51800.24829786540530074No Hit
CCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGAC51790.2482499314544503No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA51710.24786645984764674No Hit
TCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCT51300.24590116786277852No Hit
CCGCCAAGCTCGTCTCAGAGAAGCTGGCCTCCTACCAGGCTGCGCGCTGT49790.23866314128436145No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA49680.23813586782500656No Hit
CTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTC48410.23204825606700016No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGTTTCACCAACAG47880.2295077566719266No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT47860.22941188877022572No Hit
ACTGTATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAG46440.22260526774946265No Hit
GCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGG46230.22159865478160334No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG45290.21709286340166162No Hit
GTGCAGCCATATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCA44870.21507963746594294No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT44170.21172426090641183No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC43700.20947136521644094No Hit
GAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACA43550.2087523559536843No Hit
CAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCAGCTGGAGATGG41750.20012424480060437No Hit
GCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGCGACCCGGACG41590.19935730158699727No Hit
GTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGC40880.1959539910766157No Hit
CCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCA39290.1883324928913951No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT39150.18766141757948887No Hit
GTGAACCAGAGTTTCATCTGCGACCCGGACGACGAGACCTTCATCAAAAA38680.18540852188951798No Hit
CCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATG38570.18488124843016313No Hit
CACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCG38470.18440190892165867No Hit
CCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAGGCCAGCTT38250.18334736200294888No Hit
GCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAA36510.1750068545549716No Hit
GGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCA35680.17102833663438477No Hit
CATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGT35350.1694465162563201No Hit
GACGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAG34700.16633080945104123No Hit
ATGTGGAGCGGCTTCTCGGCCGCCGCCAAGCTCGTCTCAGAGAAGCTGGC34420.16498865882722877No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA33560.1608663390540906No Hit
GTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGGCCGACCA33340.15981179213538083No Hit
GCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAG33180.1590448489217737No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG33120.15875724521667106No Hit
GAGCTTCTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAG33120.15875724521667106No Hit
GCCATATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCAGCAGC33010.15822997175731615No Hit
CAGCCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAG32920.15779856619966218No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC32230.1544911235909815No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT31770.15228616185186108No Hit
GACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGG31530.1511357470314504No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTTCGCACCAAG30970.14845144578382555No Hit
CAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGC30640.1468696254057609No Hit
TCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCC30420.14581507848705114No Hit
CCTGGTAGGAGGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAG30240.14495226737174313No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC29410.14097374945115626No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG29340.14063821179520314No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG28760.1378580426458774No Hit
CTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCACGG27030.12956546914875056No Hit
CACATACAGTCCTGGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGG26810.1285109222300408No Hit
CGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACATGGTG26740.12817538457408767No Hit
GAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGC26550.12726463950792924No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTTCGCACCAAGACCCCTTTAACT26510.12707290370452745No Hit
CTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGT26120.12520347962136014No Hit
GACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCGGCTTCT25940.12434066850605215No Hit
CACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCC25580.12261504627543617No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG24790.11882826415825105No Hit
TGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAACTAA24750.11863652835484928No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA24610.11796545304294306No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA24600.11791751909209262No Hit
CGCCACCGCCGTCGTTGTCTCCCCGAACACAGCGCGCAGCCTGGTAGGAG24260.11628776476317751No Hit
CCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGC23840.11427453882745886No Hit
CGACGAGACCTTCATCAAAAACATCATCATCCAGGACTGTATGTGGAGCG23790.11403486907320665No Hit
CTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACATACAGTCCT23640.11331585981044999No Hit
TATTTCTACTGCGACGAGGAGGAAAACTTCTACCAGCAGCAGCAGCAGAG23200.11120676597303045No Hit
GTGCACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGA23000.11024808695602156No Hit
TCTGCGACCCGGACGACGAGACCTTCATCAAAAACATCATCATCCAGGAC22750.10904973818476045No Hit
CACGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTG22640.10852246472540558No Hit
ACGTTAGTTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG22640.10852246472540558No Hit
CGTGAGGTTCGCACAGACCTTCATCAAAAACATCATCATCCAGGACTGTA22390.10732411595414447No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG22360.10718031410159315No Hit
CTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTG22210.10646130483883648No Hit
CCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCC21830.1046398147065196No Hit
AAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAG21210.10166790975379206No Hit
CTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTC21130.1012844381469885No Hit
GGCCAGCTTCTCTGAGACGAGCTTGGCGGCGGCCGAGAAGCCGCTCCACA21130.1012844381469885No Hit
GATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAAC21130.1012844381469885No Hit
TGGTGACCGAGCTGCTGGGAGGAGACATGGTGAACCAGAGTTTCATCTGC21060.10094890049103539No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)