FastQCFastQC Report
Sun 15 May 2022
EGAF00005286299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697341
Sequences flagged as poor quality0
Sequence length35-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG431952.544862817783816No Hit
GTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGAT277661.6358527838542758No Hit
GCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGG228351.345339563470157No Hit
GCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTA225531.3287253415783864No Hit
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT219981.2960271389190505No Hit
CTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT191681.1292957631966705No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA150050.8840297854114171No Hit
GCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGG138460.8157465117498488No Hit
ACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG131600.7753303549493001No Hit
GCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGC130780.7704992691509838No Hit
GCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCGA123140.725487689273988No Hit
GCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTT115440.6801226153141885No Hit
TAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACAC115240.678944301704843No Hit
GCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCA109930.6476600753767217No Hit
GTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCA104930.6182022351430856No Hit
ACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAA97730.5757829452066497No Hit
GTTCTGGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGA94760.5582849881078699No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG94540.5569888431375899No Hit
ACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGC87620.5162191922542376No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT84940.5004297898890088No Hit
GCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCT82630.4868202677010689No Hit
CCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAG82170.4841101463995744No Hit
GTAGCAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG81940.48275508574882714No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG73970.4357992884164113No Hit
GGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTG72740.42855265971893686No Hit
ACTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTAC72670.4281402499556659No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTGACCAACAG69300.40828566563819524No Hit
CTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACC68630.40433831504688805No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCA64940.38259842895446466No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA63740.375528547298392No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA63540.3743502336890466No Hit
TACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAA63360.3732897514406357No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG60240.3549080591348468No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG58420.3441854052898033No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT58290.34341950144372874No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG58280.3433605857632615No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG55580.327453352037098No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA54830.32303467600205266No Hit
CCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGA54350.3202067233396236No Hit
GCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCA53170.31325467304448545No Hit
GCCTTCTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG52310.3081879245243001No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC51800.3051832248204692No Hit
GGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATT50610.2981722588448638No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT49840.2936357514488839No Hit
ACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAG49480.29151478695206207No Hit
ACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTAT48060.28314876032570946No Hit
CTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTACC47700.28102779582888765No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC47150.2777874334031877No Hit
CCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA45210.2663577913925369No Hit
GGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGT44790.2638833328129115No Hit
GGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTG44390.26152670559422064No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG42480.25027381062497167No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT41700.24567838754852445No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCG40870.24078838606974082No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGA40320.2375480236440409No Hit
CCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAAT39190.23089055175123915No Hit
GAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGG38640.22765018932553918No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC38280.22552922482871737No Hit
CAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCA37440.22058030766946654No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC37120.21869500589451382No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTCAAGCTAA37080.21845934317264473No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC37040.21822368045077567No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATC36550.21533681210787933No Hit
GCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCA35880.21138946151657212No Hit
CCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAA35360.20832584613227398No Hit
CCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCA35220.20750102660573216No Hit
GGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGG34560.20361259169489218No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA34130.20107921743479948No Hit
CAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAG33500.19736752956536133No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG33200.19560005915134318No Hit
CTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGATTA32340.1905333106311578No Hit
GCTCAGCACAGCCCAGCTCAGCCCAGCTCAGCCCAGCTCAGCCCAGTCTA31590.18611463459611238No Hit
CTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAA31230.1839936700992906No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC30700.18087113903452517No Hit
GTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGA30640.18051764495172154No Hit
CTTAAAAACCATTCCCGTTTTCCCTCTGCCTTCTCCTCTCCCATCTTGAC30590.1802230665493852No Hit
GGACAGGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCT30170.17774860796975978No Hit
GCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGA29920.17627571595807795No Hit
ATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTC29750.17527414939013433No Hit
GAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGA29750.17527414939013433No Hit
GGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTA29220.1721516183253689No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC28370.1671437854856508No Hit
CCCTGCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG27860.16413908578181993No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC27820.16390342305995084No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAG27550.16231269968733447No Hit
GAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTA27260.16060414495378358No Hit
TATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT27140.15989715678817634No Hit
ACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCC26410.15559631211406547No Hit
GCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCA26320.15506607098986No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT26210.15441799850472004No Hit
GGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGC26210.15441799850472004No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG26150.15406450442191638No Hit
GCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCA26110.15382884170004732No Hit
GGCCGGGAGTCAGCGTGAATATATTCATAAGGCAGAAATCTCGAAAGGGT25830.1521792026469637No Hit
CATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGATTACCCCCCACC25170.14829076773612374No Hit
GGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG24940.14693570708537648No Hit
CCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAG24800.14611088755883467No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCG24610.1449914896299565No Hit
CAATAAAGCAGGAATGTCCGACCGGCCGGGAGTCAGCGTGAATATATTCA24500.14434341714481652No Hit
GCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATG24270.14298835649406927No Hit
GCCCAGCTCAGCCCAGCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCA24220.1426937780917329No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG23760.1399836567902384No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC23510.1385107647785566No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG23450.13815727069575293No Hit
GCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTAATGCT23180.1365665473231366No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT23170.13650763164266932No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG23100.1360952218793984No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC22700.13373859466070753No Hit
ATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCATTCCT22650.13344401625837118No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC22140.13043931655454033No Hit
GCCCAGCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCG21850.12873076182098941No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT21520.12678654436556944No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA21300.12549039939528944No Hit
ACTCCAAGGAGCTCAGGATGCAAGGGGCTTTCTGCCCACCGCAAAGCAAC21110.1243710014664113No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT21040.12395859170314039No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA21020.12384076034220584No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCAGTTCG21000.1237229289812713No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT20270.11942208430716042No Hit
GCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCA20180.118891843182955No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC19790.11659413164473138No Hit
CAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAG19780.11653521596426411No Hit
GGCGATATGCGGTCCCTACTCCAAGGAGCTCAGGATGCAAGGGGCTTTCT19720.11618172188146048No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTCAA19360.11406075738463867No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA19270.11353051626043321No Hit
GCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCA19080.11241111833155505No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG18960.11170413016594781No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCA18880.11123280472220962No Hit
GCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCA18730.11034906951520053No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT18700.11017232247379873No Hit
GCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCA18640.10981882839099508No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC18600.109583165669126No Hit
CCTAAATAGGGTGTCTTTTCTCCCATTCCTGCGCTATTGACACTTTTCTC18370.10822810501837875No Hit
TGCCTAAATAGGGTGTCTTTTCTCCCATTCCTGCGCTATTGACACTTTTC18070.10646063460436059No Hit
CAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCA17950.10575364643875332No Hit
CCCTCAACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCG17910.10551798371688423No Hit
CTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTC17790.10481099555127696No Hit
TTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCG17360.10227762129118427No Hit
GCGTGAATATATTCATAAGGCAGAAATCTCGAAAGGGTAGTCTTAAAAAC17220.10145280176464247No Hit
CCTCAACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCGG17200.10133497040370792No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG17140.10098147632090428No Hit
CAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCG17030.10033340383576428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)