FastQCFastQC Report
Wed 11 May 2022
EGAF00005286300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697341
Sequences flagged as poor quality0
Sequence length35-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG405102.3866742157291907No Hit
GTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGAT236871.395535723228273No Hit
GCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGG227431.339919320867168No Hit
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT218951.2899588238309214No Hit
GCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTA196301.1565148075725502No Hit
CTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT174791.0297871788874482No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA139470.8216969954770432No Hit
GCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGG132940.7832250561319146No Hit
GCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCGA132530.7808095132327564No Hit
GCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGC122810.723543471818568No Hit
ACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG119720.705338526554181No Hit
GCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCA96480.5684184851482408No Hit
TAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACAC96180.5666510147342225No Hit
GCTCAGCCCAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTT94140.554632215918899No Hit
GTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCA93680.5519220946174045No Hit
CTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACC93520.5509794437299282No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG90810.5350132943232974No Hit
ACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAA87730.5168672647393777No Hit
GTTCTGGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGA86180.5077353342669505No Hit
ACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGC83590.49247617302592706No Hit
CCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAG74560.4392753135639803No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT74250.43744892746949493No Hit
GCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCAACTCT72240.42560687569557326No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTGACCAACAG65910.38831324995979005No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA65540.38613336978250096No Hit
GGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTG63430.3737021612039066No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG59680.3516087810286796No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA58900.34701335795223237No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA57540.33900082540868337No Hit
ACTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTAC56180.3309882928651343No Hit
GTAGCAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG55890.32927973813158345No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG55860.3291029910901816No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT55630.3277479304394344No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCA55170.3250378091379399No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG54150.31902840973027813No Hit
TACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAA53430.31478648073663457No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG49780.2932822573660802No Hit
GCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCA49320.29057213606458576No Hit
CCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGA47420.279378156775804No Hit
GCCTTCTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG46880.27619671003057134No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC46240.27242610648066595No Hit
CCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA45230.2664756227534715No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG44350.2612910428723515No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT44220.260525139026277No Hit
GGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATT43770.2578739334052498No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGA42020.2475636893234771No Hit
ACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTAT41880.24673886979693532No Hit
ACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAG41720.24579621890945896No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG41210.2427915192056281No Hit
CTTTACCCCGATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTACC40190.23678211979796635No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC39800.23448440825974276No Hit
GGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTG38990.22971223814189373No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT38930.22935874405909007No Hit
GGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGT37970.22370283873423194No Hit
CCCCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAAT37960.22364392305376468No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC34960.20596921891358308No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC34900.20561572483077944No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATC34880.2054978934698449No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC34140.2011381331152668No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCG33960.2000776508668559No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC33920.1998419881449868No Hit
GAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGG33490.19730861388489407No Hit
GCTCAGCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCA33330.19636596299741774No Hit
GTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGA33110.19506981802713774No Hit
CTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAA32460.19124029879676505No Hit
CAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCA32190.18964957542414873No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTCAAGCTAA31420.18511306802816876No Hit
CCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCCA30860.18181378992200153No Hit
GGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGG30470.17951607838377792No Hit
CAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAG29390.17315318489331252No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG29280.17250511240817254No Hit
CCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAA29010.1709143890355562No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA28570.16832209909499624No Hit
CTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGATTA28220.1662600502786417No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC27660.16296077217247448No Hit
GGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTA27530.16219486832639995No Hit
GCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGA25970.1530040221735055No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT25670.15123655175948733No Hit
GCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCAGCCCA25660.15117763607902007No Hit
CCCCACCCCAGCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAG25390.14958691270640373No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCG24830.1462876346002365No Hit
GAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGA24660.14528606803229288No Hit
GCTCAGCACAGCCCAGCTCAGCCCAGCTCAGCCCAGCTCAGCCCAGTCTA24510.14440233282528378No Hit
GAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTA24350.14345968193780742No Hit
GCCCCAGTTACCATAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATG24260.14292944081360198No Hit
GGACAGGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCT24230.14275269377220018No Hit
TATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT24160.14234028400892926No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT24130.14216353696752745No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG23970.1412208860800511No Hit
CTTAAAAACCATTCCCGTTTTCCCTCTGCCTTCTCCTCTCCCATCTTGAC23430.13803943933481838No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC23350.13756811389108023No Hit
ATCCAGTTCTGGATTACCCCCCACCCCAGCCCCAGTTACCATAACTACTC23070.1359184748379966No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT22980.13538823371379116No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG22780.1342099201044457No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA22530.13273702809276391No Hit
GCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCAGCCGACCTCA22270.13120522040061486No Hit
GGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGC22270.13120522040061486No Hit
GCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTAATGCT22170.13061606359594213No Hit
CCCTGCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG21830.12861293046005487No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC21670.1276702795725785No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCAGTTCGAATTTCTTCCAG21590.12719895412884036No Hit
ACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCAGCCC21380.12596172483902762No Hit
GGCAGCAGCAGTTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG21340.12572606211715853No Hit
CATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGATTACCCCCCACC21300.12549039939528944No Hit
GCCCAGCTCAGCCCAGCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCA21250.1251958209929531No Hit
ATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCATTCCT20940.12336943489846766No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG20800.12254461537192585No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC20630.12154304880398223No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA20580.12124847040164587No Hit
GGCCGGGAGTCAGCGTGAATATATTCATAAGGCAGAAATCTCGAAAGGGT20490.12071822927744041No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG19270.11353051626043321No Hit
CAATAAAGCAGGAATGTCCGACCGGCCGGGAGTCAGCGTGAATATATTCA18910.11140955176361143No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC18900.11135063608314416No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG18690.11011340679333145No Hit
GCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCAGTCTACCTCA18590.10952424998865874No Hit
GCCCGGCCCAGCCGACCTCAGCCCAGCCCAGCCCAGTCCAGCTCAGCCCA18470.10881726182305146No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA18430.10858159910118238No Hit
CAGTCTACCTCAGCCCAACTCTGCTTAGACGCTGGATTTTTTTCGGGTAG18390.10834593637931329No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT18310.10787461093557513No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC17970.10587147779968786No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT17960.1058125621192206No Hit
CTGCTGGAGCGGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGT17870.10528232099501515No Hit
CCCTCAACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCG17640.10392726034426789No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG17590.10363268194193154No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC17550.10339701922006243No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCA17490.1030435251372588No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT17410.10257219969352063No Hit
GCCCAGCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCG17330.10210087424978247No Hit
CCTCAACGTTAGCTTGACCAACAGGAACTATGACCTCGACTACGACTCGG17290.10186521152791336No Hit
GCTCAGCCCAGCTCAGCCCAGTCTAGCTCAGCCCAGCTCAGCCCGGCCCA16980.10003882543342794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)