FastQCFastQC Report
Tue 10 May 2022
EGAF00005286411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373250
Sequences flagged as poor quality0
Sequence length35-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC5420.1452109845947756No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG5290.14172806430006699No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA5090.13636972538513062No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT4560.12217012726054924No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG4450.11922304085733423No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGG4120.11038178164768922No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC4100.10984594775619559No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA3980.10663094440723375No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG3760.10073677160080374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)