FastQCFastQC Report
Tue 10 May 2022
EGAF00005286583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461041
Sequences flagged as poor quality0
Sequence length35-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCTCGGTGCT52861.1465357744755889No Hit
ATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTA52221.1326541457267358No Hit
GTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGAG43350.9402634472855994No Hit
CATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCT42440.9205255064083238No Hit
CTATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAAT38450.8339822271771925No Hit
GTTCTGGATTACCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGA34110.7398474322240322No Hit
CCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAATAGCGCAG26730.5797749007138194No Hit
GTGTAAAACCAGGTAAGCACCGAGGTCCACTTGACTTTTAATTTATTTTT24800.5379131140180592No Hit
GCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCA24700.5357441095260508No Hit
GTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGC23210.5034259425951271No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGG22500.48802601070186813No Hit
TCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCTCG19150.41536436021959No Hit
GCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTAAG18780.4073390435991593No Hit
CCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAA17790.38586589912827707No Hit
AGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGC17620.382178591491863No Hit
GCTACGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT16920.36699556004780487No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG16890.36634485870020234No Hit
CCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGG15280.3314238863788687No Hit
AAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCAC15140.3283872800900571No Hit
GTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCACCTGCAAGGAGAG14680.3184098594268189No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG14440.31320424864599894No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGG14230.3086493392127815No Hit
GTCAAGTGGACCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATC13070.2834888871054852No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA12980.2815367830626777No Hit
TACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT12790.2774156745278619No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT12120.2628833444314063No Hit
GGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTC11600.25160452107296316No Hit
ACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGA11360.2463989102921432No Hit
GGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATT11300.24509750759693824No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG11280.24466370669853657No Hit
TTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC11120.2411932995113233No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA11050.2396749963669175No Hit
GGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTG10800.2342524851368967No Hit
GGTAAGCACCGAGGTCCACTTGACTTTTAATTTATTTTTTTATCACTTTA10650.23099897839888428No Hit
GCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTC10490.227528571211671No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGC10390.22535956671966267No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG10250.22232296043085106No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC10060.2182018518960353No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGA10000.2169004492008303No Hit
GGATTACCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGT9550.20713992898679293No Hit
GTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCACC9500.2060554267407888No Hit
TATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATT9350.20280192000277633No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCG9280.2012836168583705No Hit
CTTTAATGTTGAGATGAGTCGAATGCCTAAATAGGGTGGCTTTTCTCCCA9260.20084981595996887No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG9180.1991146123663622No Hit
CTTTACCCCGATGCAGTTCTGGATTACCCCCCACCCCAGCCCCAGGTAAC9150.1984639110187597No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT9100.1973794087727556No Hit
ACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTAT8810.1910892957459315No Hit
GAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGG8790.19065549484752983No Hit
CCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAAT8780.190438594398329No Hit
TTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTA8710.18892029125392318No Hit
GCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTAAGCACCGA8500.18436538182070575No Hit
GGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGA8490.1841484813715049No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA8270.17937667148908665No Hit
GTGGTCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG8230.17850906969228333No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG8130.176340065200275No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG8020.1739541602590659No Hit
CCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT7980.17308655846226256No Hit
GAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG7920.1717851557670576No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC7910.17156825531785677No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC7870.17070065352105343No Hit
GCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATG7750.1680978481306435No Hit
CAAGTGGACCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCA7740.16788094768144265No Hit
GCCTTCCCCTCTCCCATCTTGACAAGTCACTTTACCCCGATGCAGTTCTG7680.16657954498623767No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA7650.1659288436386352No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG7550.16375983914662687No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCG7220.15660212432299947No Hit
GGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG7180.15573452252619616No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC7010.15204721488978204No Hit
ACCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATA6990.15161341399138037No Hit
TCTCTGAAAGGCTCTCCTTGCAGGTGCTTAGACGCTGGATTTTTTTCGGG6960.1509627126437779No Hit
GAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG6910.14987821039777374No Hit
AAGCCACCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAA6830.1481430068041671No Hit
ACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT6800.1474923054565646No Hit
ATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC6600.143154296472548No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC6550.14206979422654384No Hit
GGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGC6500.1409852919805397No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC6480.14055149108213805No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC6480.14055149108213805No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC6350.13773178524252724No Hit
GAATGGGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATTA6330.13729798434412557No Hit
CGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTG6290.13643038254732226No Hit
ACTTTACCCCGATGCAGTTCTGGATTACCCCCCACCCCAGCCCCAGGTAA6250.13556278075051892No Hit
GAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTGA6120.13274307491090814No Hit
GCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT6110.1325261744617073No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG6050.13122477176650232No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA5980.1297064686220965No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCA5940.12883886682529322No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGGTGCTTAGACGCTG5840.1266698623332849No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG5620.12189805245086663No Hit
GCACCGAGGTCCACTTGACTTTTAATTTATTTTTTTATCACTTTAATGTT5390.11690934211924754No Hit
GTGGACCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCG5380.1166924416700467No Hit
CCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAATAG5330.11560793942404254No Hit
CATTTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCT5250.1138727358304359No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG5250.1138727358304359No Hit
GGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT5230.11343893493203423No Hit
CAAAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCC5190.11257133313523093No Hit
CTCCCATCTTGACAAGTCACTTTACCCCGATGCAGTTCTGGATTACCCCC5100.11061922909242346No Hit
TGCCTAAATAGGGTGGCTTTTCTCCCATTCCTGCGCTATTGACACTTTTC5030.10910092594801765No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC5030.10910092594801765No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG5030.10910092594801765No Hit
CCCCTCTCCCATCTTGACAAGTCACTTTACCCCGATGCAGTTCTGGATTA4930.10693192145600934No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG4890.10606431965920601No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACC4890.10606431965920601No Hit
ACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGAC4870.10563051876080434No Hit
CCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAA4830.10476291696400104No Hit
ATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAA4790.1038953151671977No Hit
CTTAAAAACCATTCCCGTTTTCCCTCTGCCTTCCCCTCTCCCATCTTGAC4750.1030277133703944No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA4690.1017263106751894No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT4660.10107560932758693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)