FastQCFastQC Report
Sun 15 May 2022
EGAF00005286584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461041
Sequences flagged as poor quality0
Sequence length35-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGG30180.6546055556881059No Hit
ATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTA29320.6359521170568344No Hit
ACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCTCGGTGCT28860.6259746963935963No Hit
AGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGC28780.6242394927999896No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCG26260.5695805796013803No Hit
GTTCTGGATTACCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGA25710.5576510548953347No Hit
TTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC24800.5379131140180592No Hit
GTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGAG24130.5233807839216035No Hit
GCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTAAG22370.4852063048622574No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG22240.4823865990226466No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC21650.4695894725197976No Hit
CATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCT21530.46698666712938763No Hit
CTATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAAT20510.44486282131090293No Hit
CCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAATAGCGCAG20000.4338008984016606No Hit
CCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGG19580.4246910795352258No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGG18800.40777284449756096No Hit
TACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT17930.3889025054170887No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG16570.3594040443257758No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGC16330.35419843354495584No Hit
GGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTC15370.33337599042167615No Hit
GCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCA14670.31819295897761807No Hit
GTGTAAAACCAGGTAAGCACCGAGGTCCACTTGACTTTTAATTTATTTTT13870.30084092304155163No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG13820.2997564207955475No Hit
GAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG13640.2958522127099325No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA13520.29324940731952254No Hit
GTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGC12860.27893397767226774No Hit
GCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTAAGCACCGA12420.26939035790743127No Hit
ATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC11740.25464112736177474No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT11040.23945809591771663No Hit
TTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGGTA10790.2340355846876959No Hit
TCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGACCTCG10620.23034827705128175No Hit
CCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAA10580.22948067525447846No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG10560.2290468743560768No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT10230.2218891595324494No Hit
GGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT10040.21776805099763363No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG9930.2153821460564245No Hit
AAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCAC9890.21451454425962116No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC9890.21451454425962116No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA9740.21126103752160869No Hit
TCTCTGAAAGGCTCTCCTTGCAGGTGCTTAGACGCTGGATTTTTTTCGGG9490.20583852629158794No Hit
CCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT9410.20410332269798132No Hit
ACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT9400.20388642224878048No Hit
GGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG9280.2012836168583705No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC9020.1956442051791489No Hit
GGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACAC8990.19499350383154643No Hit
GGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGC8930.19369210113634147No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG8860.19217379799193562No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG8830.19152309664433315No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGG8570.18588368496511157No Hit
GTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCACCTGCAAGGAGAG8080.1752555629542709No Hit
GAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG8010.17373725980986507No Hit
GCTACGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT7580.16441054049422935No Hit
GCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAA7490.16245843645142188No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC7450.16159083465461857No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGGTGCTTAGACGCTG7440.16137393420541774No Hit
GGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATT7380.16007253151021278No Hit
GTCAAGTGGACCTCGGTGCTTACCTGGTTTTACACCACCCGAAAAAAATC7310.15855422836580693No Hit
GTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCA7300.15833732791660612No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA7120.15443311983099117No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC6900.1496613099485729No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC6870.1490106086009704No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCA6870.1490106086009704No Hit
CTTTACCCCGATGCAGTTCTGGATTACCCCCCACCCCAGCCCCAGGTAAC6800.1474923054565646No Hit
GGAGAAAAGCCACCCTATTTAGGCATTCGACTCATCTCAACATTAAAGTG6790.14727540500736377No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACC6770.1468416041089621No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCGA6670.1446725996169538No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG6610.14337119692174882No Hit
ACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTCAAGTGGA6560.14228669467574467No Hit
GCCTTCCCCTCTCCCATCTTGACAAGTCACTTTACCCCGATGCAGTTCTG6530.1416359933281422No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG6240.1353458803013181No Hit
GAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCG6130.13295997536010895No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGGCATTCG6130.13295997536010895No Hit
GTGCTTACCTGGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCACC6120.13274307491090814No Hit
ACTCTGAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTAT6110.1325261744617073No Hit
CACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCA6080.13187547311410483No Hit
ACTTTGCACTGGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGAC5970.12948956817289567No Hit
GGATTACCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGT5930.12862196637609238No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT5930.12862196637609238No Hit
CCCCCCACCCCAGCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAAT5910.1281881654776907No Hit
ACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTT5730.12428395739207576No Hit
GGTAAGCACCGAGGTCCACTTGACTTTTAATTTATTTTTTTATCACTTTA5700.12363325604447327No Hit
GCACTGGAACTTACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGG5690.12341635559527243No Hit
CCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAA5680.1231994551460716No Hit
CTGAAAGGCTCTCCTTGCAGGTGCTTAGACGCTGGATTTTTTTCGGGTGG5680.1231994551460716No Hit
GCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATTAAAAGTC5630.12211495290006746No Hit
AGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCA5470.11864454571285418No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC5380.1166924416700467No Hit
GAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGCCACCCTATTTAGG5250.1138727358304359No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC5180.1123544326860301No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG5160.11192063178762843No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC5130.11126993044002594No Hit
TAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACACCCGAGCAAGGA5080.11018542819402179No Hit
TATTTAGGCATTCGACTCATCTCAACATTAAAGTGATAAAAAAATAAATT5050.1095347268464193No Hit
GCCCCAGGTAACAAAACTACTCTGAGAAAAGTGTCAATAGCGCAGGAATG5040.10931782639721846No Hit
CCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATT4990.10823332415121431No Hit
CCTTGCAGGTGCTTAGACGCTGGATTTTTTTCGGGTGGTGTAAAACCAGG4950.10736572235441098No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA4940.10714882190521018No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC4940.10714882190521018No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC4940.10714882190521018No Hit
CGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGA4930.10693192145600934No Hit
GCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCGCATCCA4930.10693192145600934No Hit
CCCCTCTCCCATCTTGACAAGTCACTTTACCCCGATGCAGTTCTGGATTA4870.10563051876080434No Hit
GTGGTCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG4800.10411221561639854No Hit
GTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGGCTTCGCCTCTGGCCCA4780.10367841471799688No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT4760.10324461381959524No Hit
CTTTAATGTTGAGATGAGTCGAATGCCTAAATAGGGTGGCTTTTCTCCCA4730.10259391247199273No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG4710.10216011157359106No Hit
GGTTTTACACCACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGA4680.10150941022598857No Hit
CTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGC4640.10064180842918526No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA4630.10042490797998443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)