FastQCFastQC Report
Wed 11 May 2022
EGAF00005286611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705309
Sequences flagged as poor quality0
Sequence length35-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA841472.270984687107067No Hit
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT501731.354084099328828No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGG461051.2442956849212845No Hit
CATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC454201.2258086977361402No Hit
CTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT400021.0795860750075095No Hit
CCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGCAGTGGAAAACCAG367160.9909025131237368No Hit
CCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA350720.9465337438793903No Hit
GCTTAGACGCTGGATATTTTTTCGGGCAGTGGAAAACCAGGTAAGCACCG322610.8706696256641484No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG290140.7830386075763183No Hit
TATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAA280310.7565091062580745No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGCA246070.6641011586348129No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG216320.5838109588161203No Hit
AGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGC202030.5452446746006878No Hit
TTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC173300.46770728163292186No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT167100.4509745341076817No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG163700.44179851127125974No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC161840.43677868701368766No Hit
CACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT154530.4170502379153803No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA153120.4132448872685112No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT136790.3691729893512255No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG136590.3686332233020242No Hit
GCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCA136590.3686332233020242No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA134420.3627767616681901No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT133010.358971411021321No Hit
GCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTAT131590.35513907207199186No Hit
CCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAA129440.3493365870430779No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG129430.34930959874061784No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC125460.3385952426639722No Hit
ATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC122110.3295541613398505No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA120530.3252900095511603No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC115630.3120657413457285No Hit
GAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG113400.3060473498971341No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG112520.3036723792806484No Hit
GGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTG112420.3034024962560477No Hit
CCTCTGGCCCAGTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT112120.3025928471822458No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC110690.29873351993045655No Hit
GTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAG109940.2967093972459517No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT109230.2947932277712871No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG105390.2844297196266222No Hit
CTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTA105120.2837010354602005No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCG101330.273472468827836No Hit
GGCGGGCACTTTGCACTGGAACTCACAACACCCGAGCAAGGACGCGACTC98090.26472825883077494No Hit
GTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAGCTGCAAGGAGA97240.26243425312166946No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC97240.26243425312166946No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC95800.25854793756742017No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTCACAACACCCGAGC94470.2549584933402316No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG92400.24937191473099815No Hit
AGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCG89340.2411134941782183No Hit
GGCGCGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGC89110.24049276322163685No Hit
TAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACAC88880.23987203226505535No Hit
GAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGG88240.2381447809076112No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG88110.23779393297563037No Hit
GAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTCGG88040.23760501485840993No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA84950.2292656293982499No Hit
AAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAA83420.22513641912186No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC81960.22119612696269056No Hit
CATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGCTTACCCCCCCCC81770.22068334921594934No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATAT81120.21892910955604514No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGTCCTCCCGCTGATCCCCCAGCCA78840.21277577659515037No Hit
GTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCGCATCCA77510.20918633236796177No Hit
CCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGTAGTGGAAAACCAG77060.20797185875725885No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC75840.20467928585713094No Hit
GCTTAGACGCTGGATATTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCG74500.20106285332748228No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG73980.1996594615995589No Hit
GTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCGA73970.19963247329709885No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC73390.1980671517544151No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG72900.1967447249338719No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA72780.19642086530435116No Hit
CCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCA72370.19531434490348848No Hit
GGCCCAGTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG72020.1943697543173862No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGC71180.19210273691074076No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC70720.1908612749975778No Hit
ACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT69710.18813545644911128No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC69380.1872448424679291No Hit
TGCCTACATAGGGTGTCTTTTCTCCCATTCTTGCGCTATTGACACTTTTC68970.1861383220670665No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT68480.18481589524652328No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTACATAGGGTGTCTTTTCTCCCA67940.18335852691367982No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG67580.18238694802511746No Hit
GGAACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGC66710.18003896571109182No Hit
AACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTA65160.17585577882978182No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG65090.17566686071256135No Hit
GGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT64760.17477624673137923No Hit
GGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAGCTGCAAGGAG64660.17450636370677858No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA60680.1637650193276728No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA59750.16125510719888678No Hit
GCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATG59180.1597167739586631No Hit
TATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT59000.15923098451438195No Hit
GTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAA58940.15906905469962154No Hit
GTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGGCTTCGCCTCTGGCCCA58850.15882615997748095No Hit
GCCTTCTCCGCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG58110.15682902559543616No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT57820.15604636482409429No Hit
CACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAAT57530.15526370405275242No Hit
CCCCCCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAG56870.15348247609038815No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG56010.15116148207882257No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC55730.15040580960994077No Hit
GAATGGGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTA54770.14781493257377457No Hit
GAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGA54730.1477069793639343No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC54450.14695130689505248No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG53820.1452510438400684No Hit
ACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATA52640.14206642414978074No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT51960.14023121958249637No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT50960.1375323893364899No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG50910.13739744782418956No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC50120.13526537192984445No Hit
GGGGCGCGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC49410.13334920245517984No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC48900.13197279902971656No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC48720.13148700958543538No Hit
GAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATA48610.13119013825837467No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCC48440.13073133711655358No Hit
ACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTT48430.1307043488140935No Hit
CGTCTAAGCAGCTGCAAGGAGAGCCTTTCAGAGAAGCGGGTCCTGGCAGC48310.13038048918457273No Hit
CTCCACGGCCGACCAGCTGGAGATGGTGACCGAGCTGCTGGGAGGAGACA48260.13024554767227242No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG48150.1299486763452117No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT48110.12984072313537143No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT47890.12924698048125002No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGTA47820.12905806236402956No Hit
AGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCA47530.1282754015926877No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT47350.1277896121484065No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG47000.12684502156230426No Hit
CTCATAAGGCAGAAATCTCGAAAGGGTAGTCTTAAAAACCATTCCCGTTT46660.12592741927866205No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT46370.12514475850732015No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC45810.12363341356955654No Hit
GGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTCGGGGACAC45790.12357943696463643No Hit
CCCCCCCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATA45670.12325557733511563No Hit
CTTAAAAACCATTCCCGTTTTCCCTCTGCCTTCTCCGCTCCCATCTTGAC45480.1227427995883744No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA45360.12241893995885364No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAAT45010.12147434937275137No Hit
GGCCGGGAGTCAGCGTGAATACACTCATAAGGCAGAAATCTCGAAAGGGT43970.11866756591690462No Hit
GGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATA43780.11815478817016341No Hit
CACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCA43120.11637356020779914No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC43030.11613066548565856No Hit
ACTTTGCACTGGAACTCACAACACCCGAGCAAGGACGCGACTCTCCCGAC42990.1160227122758183No Hit
GGGTGGAAGAGCCGGGCGAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGG42890.11575282925121765No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG42720.11529402810939654No Hit
GAGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCG42290.11413353110361377No Hit
CCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATT41830.11289206919045079No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG41780.11275712767815045No Hit
GGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGT41710.11256820956093No Hit
CTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGC41610.11229832653632936No Hit
CTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGCAGTGGAAAACC41120.11097589971578618No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTCACAACACC40990.11062505178380536No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA40940.11049011027150502No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT40840.11022022724690436No Hit
CACCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCT40250.10862791740176056No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT40100.10822309286485958No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC40070.10814212795747938No Hit
TCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGA39670.10706259585907679No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA39640.1069816309516966No Hit
CCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGG39620.10692765434677648No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC39590.10684668943939629No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC39420.10638788829757519No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC39050.10538932110655279No Hit
CGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTC38780.10466063694013104No Hit
CAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAG38540.10401291768108947No Hit
GCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAA37320.10072034478096159No Hit
CAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTG37230.10047745005882101No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG37160.10028853194160056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)