FastQCFastQC Report
Wed 11 May 2022
EGAF00005286612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705309
Sequences flagged as poor quality0
Sequence length35-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA761122.054133676840447No Hit
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT487981.3169751834462389No Hit
CATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC433781.170698584112688No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGG396571.070275110658787No Hit
CTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT369680.997703565343673No Hit
CCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA338850.9144986288592936No Hit
CCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGCAGTGGAAAACCAG325520.8785232216800273No Hit
GCTTAGACGCTGGATATTTTTTCGGGCAGTGGAAAACCAGGTAAGCACCG310580.8382026978046905No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG266740.7198859798197667No Hit
TATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAA258270.6970268876360919No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC236910.6393798735813936No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGCA212250.572826719714874No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG210320.5676179773400815No Hit
AGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGC193500.5222236526022526No Hit
TTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC181660.49026950248953594No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA176410.476100643698002No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCG155430.41947918513678617No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT155100.418588571155604No Hit
CACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT140650.3795904741008105No Hit
GCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTAT139540.3765947725277433No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA139500.376486819317903No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG135110.3646389545379346No Hit
GCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCA131460.354788224140011No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT128580.34701559303151236No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG128270.34617895565525036No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG127470.3440198914584452No Hit
ATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC125130.33770462868279005No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC122180.32974307945707093No Hit
CCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAA121670.32836667603160763No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT120830.32609965862496215No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC117340.3166807410663996No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA117340.3166807410663996No Hit
GTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAG115440.31155296359898726No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC112250.30294369511422664No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT108190.29198644431544035No Hit
CCTCTGGCCCAGTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT107540.2902322046555362No Hit
GGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTG107390.2898273801186352No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG106870.2884239883907118No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC106140.2864538423111271No Hit
GGCGGGCACTTTGCACTGGAACTCACAACACCCGAGCAAGGACGCGACTC104550.2821627022199768No Hit
GAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG104400.28175787768307586No Hit
CTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTA102020.2753346616975804No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC97770.2638646331520529No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG97210.26235328821428927No Hit
GTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAGCTGCAAGGAGA96770.2611658029060464No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTCACAACACCCGAGC92450.24950685624329846No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG86180.23258519060083788No Hit
GAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGG85640.2311278222679944No Hit
GAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTCGG84980.22934659430563012No Hit
AGTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCG84040.22680969387438404No Hit
TAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGACAC83520.22540630214646068No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA82920.2237870039988568No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATAT81640.2203325012839685No Hit
GGCGCGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGC79940.21574448986575748No Hit
AAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAA78290.2112914199598468No Hit
GTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCGCATCCA78160.21094057202786595No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC77450.20902440255320137No Hit
GTGTCAATAGCGCAAGAATGGGAGAAAAGACACCCTATGTAGGCATTCGA77400.20888946104090103No Hit
CATCTTGACAAGTCACTTTACCCCGATCCAGTTCTGGCTTACCCCCCCCC74700.20160261937668356No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGTCCTCCCGCTGATCCCCCAGCCA74310.20055007558074106No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC74140.2000912744389199No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG73970.19963247329709885No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA72910.196771713236332No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG72850.1966097834215716No Hit
GGCCCAGTCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG72410.19542229811332873No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC71890.19401890638540537No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGC71460.1928584093796226No Hit
CCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCA71130.19196779539844044No Hit
GCTTAGACGCTGGATATTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCG70840.19118513462709857No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG70380.18994367271393559No Hit
CCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGTAGTGGAAAACCAG69230.18684001793102817No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT68660.18530168469080446No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC68380.18454601222192266No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA67390.18187417027837624No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG67270.18155031064885546No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG67110.18111849780949443No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC66000.17812279623642724No Hit
GGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT65070.17561288410764123No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA62760.16937858623936627No Hit
GCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATG61810.1668146975056601No Hit
GGTTTTCCACTGCCCGAAAAAATATCCAGCGTCTAAGCAGCTGCAAGGAG61010.16465563330885494No Hit
ACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT60170.16238861590220952No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC59780.16133607210626696No Hit
GGAACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGC58840.1587991716750209No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTACATAGGGTGTCTTTTCTCCCA58100.15680203729297612No Hit
GAATGGGAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTA57820.15604636482409429No Hit
CCCCCCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAG57500.15518273914537223No Hit
GCCTTCTCCGCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG57210.15440007837403033No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT56440.15232197908460535No Hit
GTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAA56380.15216004926984497No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG56300.15194414285016447No Hit
CACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAAT55550.14992002016565958No Hit
TGCCTACATAGGGTGTCTTTTCTCCCATTCTTGCGCTATTGACACTTTTC54130.14608768121633042No Hit
TATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT53580.14460332458102684No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC53500.14438741816134634No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT52990.14301101473588304No Hit
CACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCA52900.14276812001374245No Hit
GGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTCGGGGACAC52630.1420394358473207No Hit
GGGGCGCGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC51770.13971844183575513No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG51300.1384499916201321No Hit
GTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGGCTTCGCCTCTGGCCCA51120.13796420217585093No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT50790.13707358819466878No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG50250.1356162198618253No Hit
CTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGC49390.1332952258502597No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC49110.1325395533813779No Hit
GAGAAAAGACACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGA49100.13251256507891784No Hit
AACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTA49000.13224268205431722No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT48880.1319188224247964No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC48790.13167592770265585No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC48350.13048844239441298No Hit
ACCCTATGTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATA47890.12924698048125002No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG47710.12876119103696884No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG47630.12854528461728834No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG47270.12757370572872598No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT46520.12554958304422115No Hit
CCCCCCCCCCAGCCCCAGTTATCATAACTGCTCTGAGAAAAGTGTCAATA45960.12403823810645752No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCC45770.12352546035971627No Hit
CGTCTAAGCAGCTGCAAGGAGAGCCTTTCAGAGAAGCGGGTCCTGGCAGC45010.12147434937275137No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT44580.12031385236696858No Hit
CCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGA44540.12020589915712832No Hit
GAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATA44160.11918034366364587No Hit
ACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTT44040.11885648403412508No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC43450.11726417418898127No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA43280.11680537304716017No Hit
CTCATAAGGCAGAAATCTCGAAAGGGTAGTCTTAAAAACCATTCCCGTTT43250.11672440813977998No Hit
ACTTTGCACTGGAACTCACAACACCCGAGCAAGGACGCGACTCTCCCGAC43000.11604970057827836No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG42890.11575282925121765No Hit
GCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAA42850.11564487604137738No Hit
TCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTG42680.11518607489955629No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT42580.11491619187495564No Hit
GGGTGGAAGAGCCGGGCGAGCAGAGCTGCGCTGCGGGCGTCCTGGGAAGG42540.11480823866511537No Hit
CTTAAAAACCATTCCCGTTTTCCCTCTGCCTTCTCCGCTCCCATCTTGAC42370.11434943752329427No Hit
AGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCA42340.11426847261591408No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC42050.11348581184457221No Hit
GGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATA41800.11281110428307058No Hit
CACCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCA41590.11224434993140923No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATATTTTTTCGGGTA41310.11148867746252741No Hit
GGACTTCGGTGCTTACCTGGTTTTCCACTGCCCGAAAAAATATCCAGCGT40880.11032818045674463No Hit
GGCCGGGAGTCAGCGTGAATACACTCATAAGGCAGAAATCTCGAAAGGGT40810.11013926233952417No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC40600.1095725079878628No Hit
CCCGAGCAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATT40460.10919467175342192No Hit
GAGGCTATTCTGCCCATTCGGGGACACTTCCCCGCCGCTGCCAGGACCCG40410.10905973024112159No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG40140.10833104607469984No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTCACAACACC39290.10603704036559435No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT38870.10490353166227162No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA38870.10490353166227162No Hit
CTGCTGGAGCGGGGCACACAAAGCGGGAGGCAGTCTTGAGTTAAAGGGGT38700.10444473052045052No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC38350.10350013993434826No Hit
CAGTTATCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAG38150.10296037388514696No Hit
GCACTGGAACTCACAACACCCGAGCAAGGACGCGACTCTCCCGACGCGGG37730.10182686518182425No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC37520.10126011083016288No Hit
TCATAACTGCTCTGAGAAAAGTGTCAATAGCGCAAGAATGGGAGAAAAGA37350.10080130968834178No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA37280.10061239157112133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)