FastQCFastQC Report
Sun 15 May 2022
EGAF00005286649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126899
Sequences flagged as poor quality0
Sequence length35-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT176771.5686410228423309No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT156041.3846848741546491No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGG148721.3197278549364229No Hit
CCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGG135251.2001962908832113No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG107990.9582935116634232No Hit
CTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT107930.9577610770796673No Hit
GCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCGA102690.9112617900983141No Hit
AGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGC98440.8735476737489339No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA92230.8184406943301927No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAG88580.7860509238183724No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG72350.642027368912387No Hit
CATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCA69330.615228161530004No Hit
TTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC68250.6056443390223969No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC55920.4962290320605484No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA55630.49365559823906136No Hit
GCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCA53540.47510912690489565No Hit
GTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGC53260.47262443218070116No Hit
CTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAA48350.42905353541000574No Hit
GGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTC45040.3996808942061356No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATC41200.3656050808457546No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCG40990.36374155980260875No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAAC40930.3632091252188528No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG38910.34528382756573567No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC38460.34129056818756603No Hit
ATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC38400.34075813360381013No Hit
GGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTG37820.3356112659608359No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCG35930.3188395765725234No Hit
GTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGAG35200.31236162247015925No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG34930.30996566684325744No Hit
TAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACAC33720.29922823607084575No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGAGTCGGTGCAG33060.29337145564953027No Hit
GAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG32610.28937819627136063No Hit
GGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT32320.2868047624498735No Hit
ACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT29950.2657735963915134No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA29590.2625789888889776No Hit
GGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGA29160.2587632077053933No Hit
CCCCAGCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAG28060.24900190700320085No Hit
GCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGA27250.24181404012249544No Hit
CATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCA26570.23577978150659465No Hit
CCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA26310.23347256497698554No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC26150.23205273942030297No Hit
GCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATG26100.23160904393383966No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC25830.22921308830693787No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG25610.2272608281664994No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCA24330.21590222371303905No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT24280.21545852822657577No Hit
GGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATT24080.21368374628072256No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA24010.21306257259967398No Hit
CCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT23320.20693957488648052No Hit
GAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCG23210.20596344481626128No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG22830.20259135911914022No Hit
GGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG22590.20046162078411642No Hit
AAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGA22370.1985093606436779No Hit
GAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG22370.1985093606436779No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG22370.1985093606436779No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCG22300.19788818696262928No Hit
GGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGC22290.19779944786533663No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG22270.19762196967075132No Hit
GTTAACATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAA22140.19646836140594678No Hit
CATGTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT21460.19043410279004597No Hit
GCATTGGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC21370.18963545091441203No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGA21080.18706201709292491No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG21040.18670706070375429No Hit
GGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACAC20950.18590840882812035No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT20620.18298001861746263No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT20150.17880928104470764No Hit
GGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTC19870.1763245863205132No Hit
TACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT19280.17108897958024633No Hit
GCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAA18950.16816058936958858No Hit
GACCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG18760.16647454652102808No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATC18440.16363489540766296No Hit
GGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATT18350.16283624353202905No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC18340.16274750443473637No Hit
CGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGA18030.15999659241866396No Hit
GCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGG17950.1592866796403227No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG17950.1592866796403227No Hit
ACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGAC17170.15236503005149532No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC16670.14792807518686235No Hit
GAAAAACTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG16340.1449996849762046No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCA16210.14384607671140004No Hit
CTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTA16120.14304742483576613No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCA16070.14260372934930282No Hit
TATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT16060.14251499025201014No Hit
GCCTTCTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG15910.14118390379262027No Hit
CATTGGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCA15770.13994155643052306No Hit
GAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGA15730.13958660004135243No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG15570.13816677448466988No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC15450.137101905317158No Hit
CAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAG15430.13692442712257266No Hit
ACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTT15380.13648073163610935No Hit
AGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGA15370.1363919925388167No Hit
AGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCA15330.13603703614964607No Hit
GAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTA15150.1344397323983782No Hit
TAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAAGGA15050.13355234142545164No Hit
CCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAA14740.1308014294093792No Hit
CCCCCAGCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCA14700.13044647302020856No Hit
ATTAAAAGGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAA14450.12822799558789208No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC14370.12751808280955082No Hit
CTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGC14290.12680817003120953No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT14110.12521086627994168No Hit
CGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCC14060.12476717079347838No Hit
GGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTA13700.12157256329094267No Hit
GCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAA13700.12157256329094267No Hit
ACTCATGTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA13680.12139508509635735No Hit
GACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTT13430.11917660766404088No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC13340.11837795578840694No Hit
TAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAA13290.11793426030194366No Hit
CACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGA13040.11571578286962718No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT12850.11402974002106667No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC12580.11163378439416488No Hit
CACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCA12540.11127882800499424No Hit
CTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCA12530.11119008890770159No Hit
CAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTG12440.11039143703206766No Hit
GTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAA12260.10879413328079977No Hit
CCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCT12080.10719682952953193No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG11850.10515583029180077No Hit
CCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTA11810.10480087390263013No Hit
GTGCTTACCTGGTTTTCCACTTACCTGGTTTTCCACTACCCGAAAAAAAT11660.10346978744324026No Hit
CTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCG11610.10302609195677696No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT11440.10151752730280177No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG11410.10125131001092377No Hit
GGTGAACAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGG11300.10027517994070453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)