FastQCFastQC Report
Tue 10 May 2022
EGAF00005286650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126899
Sequences flagged as poor quality0
Sequence length35-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTGCT170221.5105169141156396No Hit
CATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT133371.1835133405921916No Hit
TCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGG121561.0787124666895613No Hit
CCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGG116351.032479397000086No Hit
CTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAAT102250.9073572698174371No Hit
AGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGC97050.8612129392252544No Hit
GCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAACCAGGTAAGCACCGA95420.8467484663665511No Hit
GCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTG95280.8455061190044539No Hit
CTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGC90260.800959092163539No Hit
ATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTA88030.7811702734672761No Hit
TTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTC74900.6646558387220151No Hit
CTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAG73150.6491264966957997No Hit
CTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCG70190.6228597238971727No Hit
TCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCG68890.6113236412491271No Hit
CATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCA57880.5136218951299095No Hit
GTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGC55880.4958740756713778No Hit
GCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCA53530.47502038780760303No Hit
ACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA51840.4600234803651436No Hit
CTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAA47730.42355171137786085No Hit
GGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTC41380.3672023845970224No Hit
GGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATC39020.34625995763595496No Hit
ATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTC37380.3317067456799589No Hit
GGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTG37350.3314405283880809No Hit
GCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAAC36770.3262936607451067No Hit
GCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC35980.31928327205898666No Hit
GTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCG35490.31493505629164636No Hit
GTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGAG35440.3144913608051831No Hit
CGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTG33330.295767411276432No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA33330.295767411276432No Hit
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG33060.29337145564953027No Hit
GGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCT32340.2869822406444588No Hit
TAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACAC31680.28112546022314333No Hit
GAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGG31210.27695472265038834No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGAGTCGGTGCAG28930.25672220846766214No Hit
CATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGC28710.25476994832722366No Hit
CCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAA28140.24971181978154208No Hit
GCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATG27070.2402167363712276No Hit
GGTTTTCCACTACCCGAAAAAAATCCAGCGTCTAAGCAGCTGCAAGGAGA27060.2401279972739349No Hit
GCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGA26630.23631221609035058No Hit
CCCCAGCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAG26480.23498112963096074No Hit
ACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTAT25380.22521982892876824No Hit
GGAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATT25330.22477613344230496No Hit
CATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCA24540.2177657447561849No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG24160.21439365905906385No Hit
CCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTTG24120.21403870266989322No Hit
GAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCG23610.2095130087079676No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA23240.20622966210813923No Hit
CCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGC22590.20046162078411642No Hit
AGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCG22410.19886431703284857No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT22260.19753323057345867No Hit
GTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTAGGCATTCGA22130.19637962230865408No Hit
GGACGCGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACAC21900.19433862307092295No Hit
GCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAA21810.19353997119528904No Hit
CAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCA21760.1930962757088257No Hit
CCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCT20910.18555345243894972No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG20780.18439984417414515No Hit
GGCCCAGCCCTCCCGCTGATCCCCCAGCCAGCGGTCCGCAACCCTTGCCG20670.1834237141039259No Hit
CACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCA20330.1804065847959755No Hit
GGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGC19880.17641332541780586No Hit
CATGTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTT19860.17623584722322053No Hit
GTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGG19780.17552593444487927No Hit
GTTAACATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAA19190.1702903277046124No Hit
GGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAAAAAAATCCAGCGTC19160.17002411041273444No Hit
GAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG19010.16869302395334454No Hit
GCATTGGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGC18990.1685155457587592No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG18870.1674506765912473No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT18770.16656328561832073No Hit
CTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGC18180.16132767887805383No Hit
AAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGA18100.1606177660997126No Hit
GTGGAAAACCAGGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTT17780.15777811498634747No Hit
CCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGA17590.15609207213778697No Hit
TACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGGGAGGCTATTCT17160.15227629095420264No Hit
GACCTGCGCTGCGGGCGTCCTGGGAAGGGAGATCCGGAGCGAATAGGGGG16430.14579833685183854No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC16420.1457095977545459No Hit
ACTTTGCCCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATC16280.14446725039244865No Hit
GCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGACGCGGG16230.14402355490598537No Hit
GGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATT16040.14233751205742484No Hit
CGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCTGCCAGGA15820.14038525191698634No Hit
ACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGAC15790.14011903462510836No Hit
TATTTAGGCATTCGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATT15710.13940912184676713No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG15650.13887668726301114No Hit
TCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTG15610.1385217308738405No Hit
CTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAAGACACCCTATTTA15590.1383442526792552No Hit
CGACTCTCCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCC15520.13772307899820657No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC15230.13514964517671949No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC15180.13470594969025618No Hit
CTTTAATGCTGAGATGAGTCGAATGCCTAAATAGGGTGTCTTTTCTCCCA15170.13461721059296353No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG14980.132931167744403No Hit
GAATGGGAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTA14860.13186629857689108No Hit
CATTGGACTCATCTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCA14750.13089016850667184No Hit
GAGAAAAGACACCCTATTTAGGCATTCGACTCATCTCAGCATTAAAGTGA14550.12911538656081867No Hit
GAATAGGGGGCTTCGCCTCTGGCCCAGCCCTCCCGCTGATCCCCCAGCCA14470.12840547378247738No Hit
ATTAAAAGGCAAGTGGACTTCGGTGCTTACCTGGTTTTCCACTACCCGAA14370.12751808280955082No Hit
CCCCCAGCCCCAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCA14190.12592077905828294No Hit
GCCTTCTCCTCTCCCATCTTGACAAGTCACTTTACCCCGATCCAGTTCTG14180.1258320399609903No Hit
AGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGA14180.1258320399609903No Hit
GGTAAGCACCGAAGTCCACTTGCCTTTTAATTTATTTTTTTATCACTTTA14110.12521086627994168No Hit
ACTCATGTCAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGA14100.125122127182649No Hit
CAGTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAG13870.12308112794491786No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC13820.12263743245845458No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC13800.12245995426386924No Hit
GAAAAACTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTG13210.11722434752360238No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT12960.1150058700912859No Hit
ACCCTTGCCGCATCCACGAAACTTTGCCCATAGCAGCGGGCGGGCACTTT12940.11482839189670059No Hit
AGCCAGCGGTCCGCAACCCTTGCCGCATCCACGAAACTTTGCCCATAGCA12670.1124324362697988No Hit
TAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAAAA12500.11092387161582361No Hit
CGGCTGCACCGACTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA12490.11083513251853094No Hit
GGACACTTCCCCGCCGCTGCCAGGACCCGCTTCTCTGAAAGGCTCTCCTT12450.11048017612936031No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT12430.11030269793477498No Hit
CCATAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAA12270.10888287237809245No Hit
GCTCTCCTTGCAGCTGCTTAGACGCTGGATTTTTTTCGGGTAGTGGAAAA12180.10808422050245853No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT12020.10666439494577598No Hit
TAGCAGCGGGCGGGCACTTTGCACTGGAACTTACAACATCCGAACAAGGA12010.10657565584848332No Hit
GTGCTTACCTGGTTTTCCACTTACCTGGTTTTCCACTACCCGAAAAAAAT11990.10639817765389799No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA11860.10524456938909342No Hit
CAGCATTAAAGTGATAAAAAAATAAATTAAAAGGCAAGTGGACTTCGGTG11610.10302609195677696No Hit
GACCCGCTTCTCTGAAAGGCTCTCCTTGCAGCTGCTTAGACGCTGGATTT11540.10240491827572834No Hit
GTTAGCATAACTACTCTTAGAAAAGTGTCAATAGCGCAGGAATGGGAGAA11500.10204996188655771No Hit
CACTTTGCACTGGAACTTACAACATCCGAACAAGGACGCGACTCTCCCGA11360.10080761452446049No Hit
CCCGACGCGGGGAGGCTATTCTGCCCATTTGGGGACACTTCCCCGCCGCT11350.10071887542716783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)