FastQCFastQC Report
Wed 11 May 2022
EGAF00005286652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385363
Sequences flagged as poor quality0
Sequence length35-150
%GC61

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAG349421.4648504231850665No Hit
GTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAG311831.3072643450912922No Hit
ACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCG309201.2962387695289983No Hit
ACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAG295461.2386374736256076No Hit
GGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACT292961.228156888490347No Hit
ACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGG246641.0339726071042437No Hit
GGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGC200540.840710617210043No Hit
GTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCA187720.7869661766364281No Hit
GTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGG162970.6832083837973507No Hit
CGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAA152840.6407410528292758No Hit
ATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTC145150.6085027729532151No Hit
GCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGC141300.5923626718449142No Hit
GGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGG140990.5910630792881418No Hit
GTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGG136630.5727849388122479No Hit
CTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACG135070.5662450536878454No Hit
CCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCT131660.5519495355633502No Hit
GGATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAA129780.5440681355416346No Hit
GGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGC128090.5369832599901986No Hit
CAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGC122660.514219429076413No Hit
GTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCACCAAGACCACC121080.5075956992709286No Hit
CTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTC119360.5003850566978695No Hit
GTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCT118680.4975343375410787No Hit
GGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAG115380.483699965162535No Hit
CTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATA109470.45892386190277956No Hit
GGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGT106610.44693407250804174No Hit
CACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCT105040.44035226504309827No Hit
GTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGG103130.4323450979997594No Hit
GCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGC102440.42945245650242747No Hit
GGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATA101960.42744018415645757No Hit
GGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCC99850.41859457030229785No Hit
GCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAA99580.4174626671076897No Hit
CCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCG96700.4053890330318698No Hit
GGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGC95230.3992264489723367No Hit
GGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTC94490.3961241957722996No Hit
CCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGG85390.35797486587995203No Hit
AAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAA83650.3506803786258108No Hit
GGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGGCT83060.3482069605338894No Hit
GCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCC82420.34552393073926274No Hit
GTGGCGCGCACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAA80060.335630258371577No Hit
CTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGAC79070.3314799466580139No Hit
GCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTGCCTCCCGCT77690.32569466366335015No Hit
TCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTA75950.318400176409209No Hit
CTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCAC75780.31768749662001133No Hit
CACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTGCAAGGGGAGAGGT75410.31613637001999273No Hit
GCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCC74970.314291787036187No Hit
CTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCG70160.294127141235946No Hit
CTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGG67730.28394001248447304No Hit
CGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACG67260.2819696624790441No Hit
GGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCG65490.2745494082032797No Hit
CCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACTC64090.26868028052753395No Hit
CTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGC63920.26796760073833625No Hit
GACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTT63690.2670033869058923No Hit
GGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTC63300.2653684156247917No Hit
CTTCCAGATATCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCT60900.2553070538949418No Hit
CTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTC59680.25019252834893474No Hit
CCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCC59060.24759334323539017No Hit
GTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGA58500.24524569216509184No Hit
GATGAAACTCTGGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCT55480.23258514532169738No Hit
CCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTTCTCCAC54870.23002788254869386No Hit
AGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGAT54400.2280575325432649No Hit
CCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGCCT54340.22780599850001867No Hit
CCAAGACCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCC53820.2256260367918845No Hit
CAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTT53800.22554219211080243No Hit
CCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTG52940.22193687082427285No Hit
GTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGA52760.22118226869453414No Hit
ATGATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACT52520.22017613252154913No Hit
GTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCA51350.2152712186782473No Hit
CTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAG51050.21401354846201603No Hit
TATTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAG50810.21300741228903108No Hit
ACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTA50690.21250434420253855No Hit
GCAGGGGACTGTCCAAAGGGGGTGAAAGGGTGCTCCCTTTATTCCCCCAC50370.21116282930522526No Hit
GGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGC49710.2083959548295165No Hit
CTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATC49240.20642560482408756No Hit
GCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATAT49220.20634176014300548No Hit
CCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGG49180.20617407078084132No Hit
AACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGA48470.20319758460242737No Hit
CAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCG48390.20286220587809903No Hit
ACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCTACCAG47920.20089185587267014No Hit
TTTCTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCG47270.19816690373750243No Hit
GGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGG46830.1963223207536966No Hit
GTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGTGGCGGGAGCTT46360.19435197074826768No Hit
CCCTTTATTCCCCCACCAAGACCACCCAGCCGCTTTAGGGGATAGCTCTG46020.19292661116987225No Hit
CTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCCCA44990.188608610094145No Hit
GAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACG44360.1859675026400594No Hit
CCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGA44210.18533866753194378No Hit
CCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCT43820.18370369625084318No Hit
CCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACAACGACGGCGGT43550.18257179305623505No Hit
CTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCT42800.17942761751565695No Hit
CACTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGAC41570.17427116962910888No Hit
GTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCTCC41010.17192351855881055No Hit
AGCTTCACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTA40770.17091738238582554No Hit
GCTCGAATTTCTTCCAGATATCCTCGCTGGGCGCCGGGGGCTGCAGCTCG40250.1687374206776914No Hit
GGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGCGCTGCGCCAGGTTTCC40220.16861165365606828No Hit
GATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGT40100.16810858556957578No Hit
GCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGC39880.16718629407767288No Hit
AACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTG39820.16693476003442664No Hit
CGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAAC39320.16483864300737455No Hit
GCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGTGGGCAGCAGCTCGAAT38810.16270060363978145No Hit
TATCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGG38610.16186215682896063No Hit
GGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGGGGCGGGGGGGGCAG38510.1614429334235502No Hit
AGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTA38240.1603110302289421No Hit
GCTTTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCA36850.15448382489373735No Hit
CACCAACAGGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCT36660.15368730042345757No Hit
GTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCA36340.15234578552614425No Hit
GCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAG36070.15121388233153613No Hit
TGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGC35980.15083658126666674No Hit
GAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGC35130.14727318232067824No Hit
CCCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCC35120.1472312599801372No Hit
CTGCGCGCTGCGCCAGGTTTCCGCACCAAGACCCCTTTAACTCAAGACTG35100.14714741529905512No Hit
GCACCGAGTCGTAGTCGAGGTCATAGTTCCTGTTGGTGAAGCTAACGTTG35050.1469378035963499No Hit
GCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTC35040.14689588125580885No Hit
CGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGAC34990.14668626955310365No Hit
GCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGACT34750.14568013338011868No Hit
GACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGA33910.14215865677467118No Hit
CTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACTTCT33760.14152982166655556No Hit
ATGATGTTTTTGATGAAGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTG33760.14152982166655556No Hit
CCCCGCCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTAC32930.13805026740164913No Hit
AGCTAACGTTGAGGGGCATCGTCGCGGGAGGCTGCTGGAGCGGGGCACAC32500.13624760675838438No Hit
GCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAAAT32410.135870305693515No Hit
ATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACGA32410.135870305693515No Hit
AGGTCTCGTCGTCCGGGTCGCAGATGAAACTCTGGTTCACCATGTCTCCT32110.13461263547728375No Hit
GTCACCATCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTC31540.13222306206644438No Hit
TGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAG30210.1266473907744859No Hit
CCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCGGCTAGGGGACAGG30070.12606047800691134No Hit
CTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTC29790.12488665247176216No Hit
GCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATG29770.12480280779068008No Hit
GCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGGTGTGACCG29610.1241320503420234No Hit
GAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCGGCG29460.12350321523390781No Hit
GCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTT29390.12320975885012052No Hit
GATATCCTCGCTGGGCGCCGGGGGCTGCAGCTCGCTCTGCTGCTGCTGCT29020.12165863225010198No Hit
GTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCGGCCGTGGAGA28950.12136517586631469No Hit
GGTTCACCATGTCTCCTCCCAGCAGCTCGGTCACCATCTCCAGCTGGTCG28910.12119748650415052No Hit
AGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCGGAGCG28770.12061057373657594No Hit
AGGTCATAGTTCCTGTTGGTGAAGCTAACGTTGAGGGGCATCGTCGCGGG28260.11847253436898283No Hit
GGCGGGGTGGGCAGCAGCTCGAATTTCTTCCAGATATCCTCGCTGGGCGC28200.11822100032573658No Hit
TCTCCAGCTGGTCGGCCGTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCT27870.11683756308788222No Hit
CCCCTGTCCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGC27740.11629257266084869No Hit
CTACTGCGACGAGGAGGAGAACTTCTACCAGCAGCAGCAGCAGAGCGAGC27550.1154960481905689No Hit
TCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGTCATAGTTCCT27070.1134837758445989No Hit
TTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAG26750.11214226094728558No Hit
GGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTGCGC26680.1118488045634983No Hit
GGCTGCAGCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTC26030.10912385242833063No Hit
ACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAG25730.10786618221209937No Hit
GTGGAGAAGCTCCCGCCACCGCCGTCGTTGTCTCCCCGAAGGGAGAAGGG25520.10698581306073751No Hit
TTAGGGGATAGCTCTGCAAGGGGAGAGGTTCGGGACTGTGGCGCGCACTG25470.1067762013580323No Hit
CCCCTAGCCGCCGCTCCGGGCTCTGCTCGCCCTCCTACGTTGCGGTCACA25450.10669235667695022No Hit
CTGCTCGCCCTCCTACGTTGCGGTCACACCCTTCTCCCTTCGGGGAGACA25340.10623121093099877No Hit
CCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCGAGCAGAGCCCG25290.10602159922829354No Hit
GCTCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAG25270.10593775454721148No Hit
GCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAACGTTAGCTTCAC25200.10564429816342419No Hit
GATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACCTCGACTACG25070.10509930773639063No Hit
AAGTTCTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTC25040.10497354071476751No Hit
CCCCTTTAACTCAAGACTGCCTCCCGCTTTGTGTGCCCCGCTCCAGCAGC24900.10438662794719294No Hit
TCGCTCTGCTGCTGCTGCTGGTAGAAGTTCTCCTCCTCGTCGCAGTAGAA24860.10421893858502877No Hit
TCCCGCGACGATGCCCCTCAACGTTAGCTTCACCAACAGGAACTATGACC24790.10392548220124148No Hit
GGAACTATGACCTCGACTACGACTCGGTGCAGCCGTATTTCTACTGCGAC24640.10329664709312586No Hit
GTCGTTGTCTCCCCGAAGGGAGAAGGGTGTGACCGCAACGTAGGAGGGCG24310.10191320985527151No Hit
CGACTACGACTCGGTGCAGCCGTATTTCTACTGCGACGAGGAGGAGAACT24070.1009070736822865No Hit
CCCGCTTTGTGTGCCCCGCTCCAGCAGCCTCCCGCGACGATGCCCCTCAA23910.10023631623362986No Hit
CTCCTCCTCGTCGCAGTAGAAATACGGCTGCACCGAGTCGTAGTCGAGGT23900.1001943938930888No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)