FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005286771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26610016
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT695160.26123997820970873No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC629910.23671913613280052No Hit
GGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAACCG489770.18405475592348383No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG439100.16501305373134686No Hit
GGTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGT414520.1557759303865131No Hit
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA367050.13793678290159617No Hit
GTCCGCGCAGTCCGCCCGGAGGATTCAACCCGGCGGCGGGTCCGGCCGTG359520.13510702135616906No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA347660.13065005297253485No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG341860.12847042256569857No Hit
GGAGTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGC330520.12420886932198764No Hit
GTTACAGCCCCCCCGGCAGCAGCACTCGCCGAATCCCGGGGCCGAGGGAG294310.1106012112131011No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC291510.1095489758442836No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA283870.10667787648079581No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG278600.10469741919734282No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA274060.10299129470647443No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA270520.10166096856161227No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG267160.10039828611903127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCTA134850.040.5850522
AGTCTAA144250.037.2440153
GGCGCGT194800.036.4374354
TGGCGCG202150.035.1843343
TAACACG164650.034.7871974
GTCTAAC170650.034.4559561
ACGTGCG174700.033.4046758
AACACGT180600.032.112745
CGCGTGC227150.031.6914146
TCTAACA197700.029.3739362
GGTCGAC88200.028.5121251
ACACGTG205850.028.4554586
CCGTCGG114000.028.3618347
CTAACAC208550.027.9511643
GCGCGTG274200.026.4121135
GATTCAA425250.026.3383925
CAACCCG416350.025.8044099
ATTCAAC435650.025.7928356
CGGTGGC203600.025.5597921
GGAGTCT252300.025.4081691
Produced by FastQC (version 0.11.5)