FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005286828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26555552
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1078690.40620130961691175No Hit
GTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACGGCC813040.30616573136947034No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG744940.28052137647148134No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG655140.24670547236223897No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT577680.21753643079985685No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA501690.18892094579694674No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA458900.1728075545181663No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAG430270.1620263815265448No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT413150.15557951873868034No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT403030.15176863956734923No Hit
CTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCGTCCGCTC377540.142169893512287No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG353610.13315859523462364No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG316820.11930461848430039No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA298620.11245106108131361No Hit
CCCGTGCGCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCC290200.10928034935971205No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT288960.10881340369049758No Hit
GGGGAGAGCGCGGCGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGA279520.10525859149905828No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA275390.10370336116530358No Hit
GGGGTCGGGAGGAACGGGGGGCGGGAAAGATCCGCCGGGCCGCCGACACG269590.10151926045446165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT49800.042.7999086
CGCGTTC55400.039.2587368
CCGCGTT56250.038.021077
GTCGGGA364200.036.84221
AGACCGC58800.036.3721964
CAGACCG62600.034.6275443
AGGAACG372250.034.4328047
TCGAGTT80700.032.4317782
GGAACGG398550.032.160618
CGTTCGA32900.031.731567
GTCGAGT84550.031.653851
GTCGGGT90000.030.9458541
CGAGTTT85700.030.0311763
GTCGATT28750.029.516291
ACGTTCG34200.029.465486
TCGGGAT137850.028.1900042
GAGGAAC471950.027.7732876
GTCGGTT31200.026.0361771
GCGTTCT83650.025.9137389
GAACGGG501850.025.8296459
Produced by FastQC (version 0.11.5)