FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005286889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005286889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35168333
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA681260.1937140438245964No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG571290.1624444354527694No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG494390.14057817298306408No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA477070.13565328785984823No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT433320.12321311902955423No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT422910.12025306971473455No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG417160.11861807609703878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC95200.044.0403332
GGGCGAT99900.042.732141
CGGTGGC280050.034.973371
GCGATCT124650.033.722823
GGTGGCG360450.030.9749551
CGATCTG142750.029.9460334
GGCGCGT390050.028.4462784
TGGCGCG436000.025.5790543
GCGCGTG435250.025.568575
CGCGTGC452450.024.5125896
GTGGCGC479350.023.4001392
GCGTGCC505600.022.170627
CGTGCCT536150.020.8987088
TGCGACA152250.018.39127312-13
TAGTCCC577550.018.1095916-17
TGTAGTC589500.017.79896214-15
CCTGTAG608450.017.30326712-13
GTAGTCC575150.016.0173814-15
TACTCGG683550.015.22111526-27
GTGCCTG762550.015.0115849
Produced by FastQC (version 0.11.5)