FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005292926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134102
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC151881.339209348012789No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG113761.0030843786537718No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC106330.9375699892954954No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC99820.8801677450529141No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG96140.8477191645901337No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG95510.8421641086956906No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC90970.8021324360595432No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC61890.5457181100112688No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC61730.544307302165061No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG61300.5405157560783774No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC55180.48655235596092766No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG54230.47817568437406865No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC54210.4779993333932927No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG53990.4760594726047569No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC53250.4695344863160456No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG53050.46777097650828586No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG52910.466536519642854No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC52060.4590416029598749No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC51780.4565726892290112No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA47730.42086161562187524No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG47720.4207734401314873No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC47630.4199798607179954No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG47500.4188335793429515No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC46830.41292582148695617No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC46660.4114268381503604No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG45870.40446097440970913No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC41910.3695434802160652No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG40220.35464182234049496No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC39890.35173203115769125No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG38550.3399165154457007No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG37520.3308344399357377No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT34070.30041389575188127No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC32060.2826906221838953No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG31380.27669468883751197No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG31210.27519570550091615No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG28610.2522700780000388No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG28570.25191737603848685No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG27660.24389340641317978No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG27440.241953545624644No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG27410.24168901915348004No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA26980.23789747306679648No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG26100.23013802991265334No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC26020.22943262598954942No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG25520.22502385147014997No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA25330.22334851715277815No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC25000.22043872596997446No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG24770.2184106896910507No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG24460.21567724948902303No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC24160.21303198477738333No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG23200.2045671377001363No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG22750.20059924063267676No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC22510.19848302886336502No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG21660.1909881121803859No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC21280.18763744354564227No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA20750.18296414255507884No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG20690.18243508961275087No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT20160.17776178862218744No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20160.17776178862218744No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC20140.17758543764141144No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC19590.172735785670072No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG19510.17203038174696808No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG19000.1675334317371806No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG18880.16647532585252472No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC18400.16224290231390123No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG18230.1607439189773054No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG17940.1581868297560537No Hit
GCTGCTAGACGTGAAGCACTTCTCGCCGGAGGAAATTGCTGTCAAGGTGG17590.15510068759247406No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT17500.15430710817898213No Hit
GTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGC16940.14936928071725472No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC16930.14928110522686672No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG16900.14901657875570276No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC16740.14760577090949492No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC16680.14707671796716698TruSeq Adapter, Index 6 (100% over 50bp)
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC16160.1424915924669915No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT16150.14240341697660353No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT16060.1416098375631116No Hit
CGAGTGCGAGTACTACCCGGTGGTGCCTGGGAAGTATGTGGTGACCATCA16050.14152166207272363No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG16030.14134531109194765No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG15970.1408162581496197No Hit
CTGATCCTGTAACGCTGCTGGAGGTCAGTCTGAGCTACCAAGACTGTCCC15790.13922909932263589No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT15720.13861187088991994No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG15690.13834734441875599No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC15490.13658383461099619No Hit
CCCACTCAGTGAAATGACCCGCGAAGAGATCAATGCGCTAGTGCAGGAGC15450.13623113264944423No Hit
GGACAGATGAAGCTGCTTTCCAGAAGCTGATGAGCAACTTGGACAGCAAC15280.1347321493128484No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT15250.13446762284168445No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG15210.13411492088013247No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG15170.1337622189185805No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC15090.13305681499547659No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC14940.13173418263965675No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG14940.13173418263965675No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG14930.13164600714926877No Hit
GTACGACGCGCACACCAAGGAGATCGACCTGGTCAACCGCGACCCTAAAC14890.1312933051877168No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC14830.13076425224538887No Hit
GGTTCGAGACTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTC14800.1304997257742249No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG14770.13023519930306093No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG14760.13014702381267293No Hit
GCCTCAACCTGGAGGCCATCAACTACATGGCAGCTGACGGTGACTTCAAG14760.13014702381267293No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG14740.12997067283189695No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC14660.12926526890879303No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC14590.1286480404760771No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG14480.1276781100818092No Hit
CCATTCGTTCGTGAGTGTCCCGTGCACGTCCTGACCCTGGACAGTGAAGG14310.1261791267452134No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG14250.12565007380288545No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG14030.12371021301434967No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG13930.12282845811046979No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG13810.1217703522258139No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC13640.12027136888921808No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG13450.11859603457184628No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG13450.11859603457184628No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG13220.1165679982929225No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG12990.11453996201399874No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC12930.1140109090716708No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG12910.11383455809089481No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG12690.11189469730235906No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG12560.11074841592731519No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC12560.11074841592731519No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG12410.10942578357149534No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC12400.10933760808110735No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT12120.10686869435024363No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG12050.1062514659175277No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC11820.10422342963860394No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG11730.10342985022511203No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG11720.10334167473472404No Hit
CGTCGGGACTGAATCAACCTGGGAAGTAGAAGTCGTGGCTCACATCCGCC11680.10298897277317208No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG11630.10254809532123212No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC11420.10069641002308435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)