FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005292929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204236
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG235751.957672748531019No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC109460.9089580447686334No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC93040.7726060340331962No Hit
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC81860.6797670888430507No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG76350.6340119378593565No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG60540.5027253794106803No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG60430.5018119371950349No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC54780.4548942233914282No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG49940.41470276590302896No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG49380.4100525146233795No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG49250.4089729920048894No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG42330.35150917262064907No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC39670.329420479042314No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC39130.3249363081655091No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC37360.3102381925137598No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC36200.30060552914877153No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC36050.2993599261274368No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC34000.2823366848358627No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG33960.2820045240301735No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG33460.27785251395905786No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG32750.2719566596580737No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG32640.2710432174424282No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG32320.2683859309969142No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG32320.2683859309969142No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG30060.24961884547547158No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG29520.2451346745986667No Hit
GAAATACAGCCCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGAT28760.23882361929057094No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC28530.23691369465785778No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG27640.22952311673127193No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC26870.22312902122175385No Hit
CTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAGCTGC26630.22113605638761838No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC24980.20743442315293678No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC24920.2069361819444029No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC24780.20577361912449055No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG24230.20120640804626336No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG24220.20112336784484106No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG22310.18526268937317936No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC22020.1828545235319323No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC21840.18135979990633067No Hit
CACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTC21700.18019723708641827No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA21600.17936683507219514No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG21350.17729083003663734No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA21300.17687562902952578No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT20830.17297273956267709No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG20750.1723084179512986No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20650.17147801593707546No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC20630.17131193553423082No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC19840.16475175962186814No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC19800.1644195988161789No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG19790.16433655861475657No Hit
AACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGG19670.1633400761976888No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT19120.15877286511946162No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC19060.15827462391092775No Hit
GAGTTCCAGACCAGTGTTGTCTCCCGGGACATTGACACAGCAGCCAAGTT18890.15686294048674843No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC18770.1558664580696807No Hit
GCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATG18610.1545378148469237No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG18140.150634925380075No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC17660.146648995711804No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG17660.146648995711804No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG17060.14166658362646525No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG16740.13900929718095126No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG16740.13900929718095126No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG16570.13759761375677193No Hit
GGTGAAAGGAGCCTGCCAGAAGACACTCAGCGACCTGAAGCTGGACTACC16310.1354385685197918No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC16280.13518944791552487No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC16130.13394384489419017No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG15810.13128655844867618No Hit
CAAAGTAGACAAATGGCGCCGGGCACGGTGGCTCACGCCTGTAATCCCAG15790.13112047804583155No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG15600.12954271421880761No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA15580.12937663381596298No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG15520.1288783926074291No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC15480.12854623180173988No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA15420.12804799059320598No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG15240.12655326696760436No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG15240.12655326696760436No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT15180.1260550257590705No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG14510.12049133226377555No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG14250.11833228702679542No Hit
GAATAACAGTAGTCCTTTGATAGAAGATAATGACTTGGTTTATGGCCTTA14230.11816620662395078No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG14060.11675452319977148No Hit
GGGCTCTCCAAATAGGAGCCTCCCATCTCCAGGTGTTCTCTGTGGGTCTG14050.11667148299834916No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG13880.11525979957416985No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC13870.11517675937274753No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG13710.11384811614999052No Hit
GGTCCATGGCAGACTTCTCTCGGCAGACACAAAGGGTTGGGTACGCCTGC13690.11368203574714592No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG13630.11318379453861205No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC13480.11193819151727735No Hit
CTGTGAATCACTGCAGGCTGTGTTCTTGCATCTTGTCTGCAAACAGGTCC13350.11085866889878727No Hit
CTCTGCACACTCCAGCCCACTTTCTGCCTTCAGCCATTGAGTGGAAGCTG13260.11011130708598647No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC13130.1090317844674964No Hit
GTGAAGACTTTGAACGCACTCCTCAGAGTCCGACTCTCCCACCTGCTAAG13010.10803530205042865No Hit
GGAAGGATTTCGCTGCTTCCCGAAGGTCTTGGACGAGCGCTCTAGCTCTG12980.10778618144616171No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG12840.10662361862624935No Hit
CACGTCATTTGGTGGTAGTGGCATGGGCAACTTCAAATCGATATCAACTT12660.10512889500064772No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC12660.10512889500064772No Hit
CCAGCCAGATGAGTGCTCTGTGGACCCACAGCCTAAGCTGAGTGTGACCC12580.10446457338926922No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC12470.10355113117362377No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT12450.10338505077077914No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC12410.1030528899650899No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG12370.10272072915940066No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG12370.10272072915940066No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG12350.10255464875655602No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC12340.10247160855513371No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG12280.10197336734659984No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC12250.10172424674233291No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)