FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005292930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences966273
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC179801.8607577775638977No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG148611.5379711530799267No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC144171.4920214059587715No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC137791.425994517077472No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC124921.2928023446789882No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG107251.1099347699873638No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG102931.065226907923537No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG94570.978708915596317No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC86700.897261953919855No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG82380.8525540918560284No Hit
GGAGTGGGAGGACTCCAAACGCTGGAGCAAGATGGACCAGCTGGCCAAGG76740.7941854941615879No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC71880.7438891493397828No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC68980.7138769271210104No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG68350.7073570305700356No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG63420.6563362527981222No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC60430.6253926167863534No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC59840.6192866819211548No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG58340.6037631187045484No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT57460.5946559616174725No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG54390.5628844022341513No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG52410.5423932987882306No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT50030.5177625784845483No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG49890.5163137125843318No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG47720.49385629113097435No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC45810.47408962063516213No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG45130.4670522719769672No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC43060.4456297547380503No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC43000.445008812209386No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG41500.42948524899277946No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT39600.4098220689184112No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG39240.40609641374642574No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC38300.3963683141306856No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA37030.3832250306072921No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG36050.3730829693057759No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC34650.3585943103036099No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC34200.3539372413386279No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG31910.330237934827942No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG31560.3266157700774005No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG30280.31336899613256297No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC30080.3112991877036821No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC28240.29225695015797815No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC28220.29204996931509003No Hit
TAACCCTCAGCATGCTAGGAGAGTGCACCCGTACCTATGGAAGTGGTAAA26980.2792171570560287No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC26910.2784927241059204No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG26370.27290424134794206No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG25920.2682471723829601No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG25830.2673157585899637No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA25730.2662808543755233No Hit
GCGAGAGGGCTTGAGTCTCAAGGACTCACTGATTACATACCCAAAGGCTC24620.25479341759523444No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG23450.24268503828628143No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG23130.23937334480007202No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG22990.23792447889985543No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA22830.2362686321567507No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC22720.2351302375208663No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG22140.22912779307711173No Hit
CGCGAACGAGGCAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTG21940.22705798464823088No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG21680.22436723369068573No Hit
GGACCAGTCGTTGTTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGA21220.21960667430425976No Hit
GCTCCACTCAGTGCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAGG20920.2165019616609385No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC20910.2163984712394944No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG20850.21577752871083017No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC20450.21163791185306843No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG20390.2110169693244042No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG20000.20698084288808652No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG19960.20656688120231034No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC18980.19642481990079408No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC18600.19249218388592043No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG18490.19135378925003596No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG18330.18969794250693126No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG18190.18824907660671467No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG17590.18203965132007208No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG17230.17831399614808652No Hit
CTCCTCACAGCCCACTGGTCCTCAAGAGGTGCCACGTCTCCACACATCAG16910.17500230266187713No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG16580.1715871187542237No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG16390.16962080074678687No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC16230.1679649540036822No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC16230.1679649540036822No Hit
CAACGTGGTCACCACGCTCGTGCTCATCTCCTACTGTCCCACGGCCACCG15970.16527420304613707No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC15950.165067222203249No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC15840.1639288275673645No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT15760.16310090419581216No Hit
CCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTCTGAGCATTGCCAATG15570.16113458618837534No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC15540.16082411492404322No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC15480.16020317239537893No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG15460.15999619155249084No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG15370.15906477775949449No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG15150.15678798848772552No Hit
GGAGCAAGAGGTCAATTAGGAACTGGGCACACTTGTAATGTTAAGTGCCC15060.15585657469472913No Hit
GCGATGAGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGAC15060.15585657469472913No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG14750.15264837162996378No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG14690.15202742910129952No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG14640.1515099769940793No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC14350.14850875477220207No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC14090.14581800381465693No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG13740.14219583906411543No Hit
CACGCAGCTTCGGTTGCAAAGACTTTCTTGATGTCGGACTGCGTTGTTGT13690.1416783869568952No Hit
GCGGACATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGG13610.14085046358534287No Hit
CGAGTGTTGTCTCGACCCAATGCTCAGGAGCTTCCTAGCATGTACCAGCG13400.13867716473501795No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG13200.1366073563061371No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC13130.13588292335602878No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG13130.13588292335602878No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG13120.13577943293458472No Hit
GGTGGTGTCTGAGGAGGTCTGCAGTAAGCTCTATGACCCGCTGTACCACC13020.1347445287201443No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG13010.13464103829870028No Hit
CATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGCGCT12870.13319217239848366No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG12730.13174330649826704No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG12610.13050142144093854No Hit
GTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTG12400.12832812259061363No Hit
GTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCG12310.12739670879761722No Hit
CAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGACACCAG12220.12646529500462084No Hit
GGCTGGAGTACGATGACCTGAGGAGAGGACGAAGCGAAGCGGACAGTGAT12200.12625831416173275No Hit
CCGCCACCGCATCGCGCAGCTCTTTGCCGTCGGAGCGCTTGTTTGCTGCC12170.12594784289740063No Hit
GCGTTTCCCGCAACCCGATACCATCGGACAGGATTTCTCCGCCTCAGCCC12150.12574086205451254No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC12130.12553388121162445No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG12090.1251199195258483No Hit
GCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAAC12070.1249129386829602No Hit
GCACTGCTTTCATTTAATTGGGCTTTAGGCACTCCATTCGAATGCAGAAC12030.12449897699718404No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC11950.12367105362563167No Hit
CCTACTTCGGCACTGGTTTCCCTCACATGCTCTTCATGGTGCATCCCGAG11940.12356756320418764No Hit
CCACTGTAGGGACATCGAACCTCAAAGATTTATGTCCCAGCGAGGGTGAG11860.12273963983263529No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG11800.12211869730397104No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC11790.12201520688252698No Hit
CGGCGAGAGCGTATGGAGCCGAGCCGTTAGCGCGCGCCGTCGGTGAGTCA11630.1203593601394223No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG11570.11973841761075804No Hit
GTTTGGAGATGTCCCAGAATAAGGCACAATGTCAATAGCAGGAGTTGCTG11540.11942794634642591No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG11490.11891049423920569No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG11410.11808257086765334No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC11380.11777209960332122No Hit
GAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAGCACCAGCTGCG11300.11694417623176886No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC11290.11684068581032482No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC11290.11684068581032482No Hit
GGTCATCCGGACATCCCAACGCATGCTCCTGGAGCTCACAGCCTTCTGTG11130.11518483906722014No Hit
CTATCGCCCTCACAAAGCCAATGCTGAGGAGATGACCAAGTACCACAGCG11100.114874367802888No Hit
GTGTTTGAGCCGTCGTGCTTCACCGGTCTACCTCGCTAGCATGTCGGGCC11080.11466738695999992No Hit
GAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGC11060.11446040611711183No Hit
CACACCATGGGACAGCGGCCTAGTTATGCCACACTCCGTATGCAGCCCAG10980.1136324827455595No Hit
GTGCACGGAGAGAGACGAGTTCTCCTACCAGGAGATGATCGCCAACCTGC10910.11290804979545117No Hit
GCCCTGCACATATCGCATGATGAGCTATGAACCACTGGAGCAGCCCACAC10820.11197663600245478No Hit
CCGCAGGATATACGCCAACATGTTCAAGAAGTTTGCAGAGCAGGATGCCA10810.11187314558101075No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG10650.11021729883790607No Hit
GCCCTAGACTCATCTTGGCCCTCCTCAGCTCCCTGCCTGTTTCCCGTAAG10640.11011380841646201No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC10590.10959635630924179No Hit
TGTCGTGTCATACTCGAGTCTGAGGCCGTTGCTGACTTAGAATCCCTGTG10430.1079405095661371No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG10410.10773352872324903No Hit
CTTTCACTGCCACATCTTCTATTGGTTCAATGGTTGCTCCGAATGGTCAC10330.10690560535169667No Hit
GAAGCCTAGGAAAGACCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGG10240.10597419155870028No Hit
GTATCAATGGGTGGGCCTGATAGTCCTGATTCAGTGGATCCCGTTGAACC10180.10535324903003603No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG10150.10504277776570388No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG10080.1043183448155956No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT10070.10421485439415154No Hit
GAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCTACTGC10070.10421485439415154No Hit
GCTCACTTTGTGCACTGAGCGATGTCACCATTTCTACCTGCCACGCATCG10060.10411136397270751No Hit
CTGGAGAGTCCAGGAGAGAAGACTCACCTCTGTCGCTTGGGTTAAACAAG10000.10349042144404326No Hit
GAGGCTAGCAGTTCAACTTCTGTAACACCAGATGTTAGTGACAATGAACC9990.1033869310225992No Hit
GCAGTTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCG9940.102869478915379No Hit
CCCATCCACGTATGCGGCGCTATATCAAGAACCCTCGCATTGTGCTGCTG9870.10214504596527069No Hit
GGTAGCCGGACTCAGCTGCGAGGAGATGGGCTTCCTCAGGGCACTGACCC9870.10214504596527069No Hit
ATGGCTTCCAGAGGCCGACGATCTACTATGTGATGTCAGGGCCTGCGTGG9850.1019380651223826No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC9810.10152410343660644No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC9780.10121363217227429No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG9690.1002822183792779No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)