FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005292950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949868
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC251382.6464729836145655No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA217672.291581567123011No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG158821.6720217967128064No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC127121.3382912152004278No Hit
CACCCTCAGCTCTGGCCTCTTACTCACCCTCTACCACAGACATGGCTCAG114571.206167593813035No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC108951.147001478100115No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG104881.1041534192119327No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC86220.9077050705992833No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC80660.8491706216021595No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG77930.8204297860334279No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC70470.7418925577027544No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG59020.6213494927716272No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC56560.5954511574239789No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC55900.5885028235502197No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG49460.5207039293880834No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC48510.5107025397213086No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG43720.4602744802435707No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC42640.4489044793592373No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC42490.44732531256974656No Hit
GGGCTTGTCCTCCTTTCTGTTACGGTCCAGGGCAAGGTCTTTGAAAGGTG42130.44353531227496873No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC40880.4303755890292125No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC40530.4266908665204007No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG37590.3957391974463821No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG36370.38289530755852397No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG36090.37994752955147454No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC35800.3768944737584591No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG34310.3612080836495176No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG31870.33552030387380144No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG31830.3350991927299372No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG30310.3190969692630976No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC30160.31751780247360684No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG29990.315728080112184No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG29380.30930613516825495No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA28950.3047791903717148No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC28120.29604113413653266No Hit
GCAACGAGACAATGACTGGGTGGGTGCATGATGTGTTGGGCCGGAACTGG27820.29288280055755117No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG27190.28625030004169No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG26470.2786702994521344No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC26050.2742486324415603No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT25990.273616965725764No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT24320.2560355754694336No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG23470.2470869636623194No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG23170.24392863008333787No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG23000.24213890772191501No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT22520.23708557399554467No Hit
CTTCGACACGGTCATCACCAACCAGGAAGAACCGTACCAGAACCACTCCG22220.23392724041656315No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG21720.22866335111826064No Hit
GACTGCAGAGGACCTCAGCCAAGATGAAGCGCCGCAGCAGTTAACCTATG21440.22571557311121127No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC21280.22403112853575444No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT20710.21803029473568958No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG19250.20265973798464632No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG19020.20023834890742714No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG18580.19560612632492091No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC18560.19539557075298883No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA18390.19360584839156597No Hit
CAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGACACCAG18320.19286890388980363No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC18300.19265834831787154No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG18090.19044751481258448No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC18070.1902369592406524No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA17990.189394736952924No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT17740.18676279230377274No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG17360.18276223643706285No Hit
CCTGGGACAATGAGATGCGGGTCACAGAGTACCTTGTCGTGTACACGCCC16940.17834056942648874No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC16810.17697195820893008No Hit
GAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGC16470.17339251348608437No Hit
GAGTACACCTCATGTGGCAGAGCCTGCTGAGAATGAGCAGGATGAGCAGG16300.17160279112466154No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG16220.17076056883693314No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG16150.1700236243351708No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG15610.16433862389300408No Hit
CAGCCAGTCATCTTTCAACAGCCTGCAGCGTGTTCCCTCCTATGACAGCT15300.16107501252805653No Hit
GATGAACGAAGCGTGCAAATCCACGTGGAACCTAAGTGCTCAGACCGAGG15240.16044334581226022No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC15030.1582325123069732No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG15030.1582325123069732No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT14860.15644278994555033No Hit
CATTGGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCG14790.15570584544378796No Hit
GACTCCATAGCCATTATCCCACCTCCACAAAGTACCAAACCAGGAAGAGG14670.15444251201219536No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC14620.15391612308236513No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC14370.15128417843321387No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG14060.14802056706826633No Hit
GTACGACGCGCACACCAAGGAGATCGACCTGGTCAACCGCGACCCTAAAC13980.14717834478053793No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC13940.14675723363667373No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT13820.14549390020508113No Hit
GCAAGTTCCAACCTGGAAAGGCCATCGGCTTTGTCAAGGATGTAGCCAAC13740.14465167791735273No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC13510.14223028884013358No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG13080.1377033440435934No Hit
CGTGTTGAGAGATCGAGGGCGACCAGAAATTCCCAGCTTCTGGCTCAACC12990.13675584396989898No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG12950.13633473282603478No Hit
CGCCCGTGATACACCAAGAAATGATTGGTGGATTACGGAACAACAACGAG12650.13317639924705327No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG12570.13233417695932487No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC12530.13191306581546067No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC12360.1301233434540378No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG12280.1292811211663094No Hit
GCACTGCTTTCATTTAATTGGGCTTTAGGCACTCCATTCGAATGCAGAAC12050.1268597320890903No Hit
GCCATAGTCGTGCCTGTTTGCTTAGCATTCCTATTGACAACTCTTCTGGG12020.12654389873119212No Hit
GGGTTCAGGATTCCATGGACCACCTGGACAAGCAAACCCAAACTCCGAAG11910.12538584308556558No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG11870.12496473194170137No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG11850.12475417636976928No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC11780.12401723186800692No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC11680.12296445400834644No Hit
GAAGCTGTTCGGGCCCTTTACCAGGAATTACTATGTTCGGGCCGTCCTGC11650.12264862065044828No Hit
CTCTGAACTCAGACGTGCAGTACACGGAAGTTCAAGTGTCCTCAGCTGAG11560.12170112057675382No Hit
GGACAGATGAAGCTGCTTTCCAGAAGCTGATGAGCAACTTGGACAGCAAC11520.12128000943288962No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG11440.12043778714516122No Hit
CCAGGACGGAGACCTCACCCTGTACCAGTCCAATACCATCCTGCGTCACC11430.12033250935919518No Hit
GGCAAGAAGGCAGCTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTAC11410.12012195378726306No Hit
CCCACTCAGTGAAATGACCCGCGAAGAGATCAATGCGCTAGTGCAGGAGC11360.11959556485743282No Hit
GCTGCAGATTCGTTCAGAGTCTAAATTCCGTTAACAACTTGGAAGCTACC11340.11938500928550073No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT11260.11854278699777233No Hit
AGAGCCAACATGTTGGTATTGCTGGCTGGTATCTTTGTGGTCCACATCGC11140.11727945356617972No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG11110.11696362020828158No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG11070.11654250906441736No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC10710.11275250876963958No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG10700.11264723098367352No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG10690.11254195319770748No Hit
GATCAGCAATGAGGCGGTGGTCAATATCCTGTCGAGCTCATCACCACAGC10620.11180500869594512No Hit
GCTGCTAGACGTGAAGCACTTCTCGCCGGAGGAAATTGCTGTCAAGGTGG10580.11138389755208092No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC10510.11064695305031857No Hit
GAAATACAGCCCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGAT10430.10980473076259017No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG10380.10927834183275993No Hit
GTTCCAGGAGCCGAATCAGAAATGTCATCCTCAGGCACGCCAGACTTACC10350.10896250847486177No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG10320.10864667511696362No Hit
GGGATGGACTATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGC10300.10843611954503153No Hit
GGGAGCACCAACTTCACCAGAACATGGCAAGACTACAAAGCAGGCTTTGG10250.10790973061520126No Hit
GGCATGGGTGGCTACGTAAAGATGGCCAAAGACCGGAGAAACCATTGTGG10230.10769917504326917No Hit
GTTGATGCCAGACATCACCAGGTTGTAGAAGTTGACAGGCAGTGCCATGG10170.10706750832747287No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG9960.10485667482218583No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT9860.10380389696252532No Hit
TCAACAATTCCTCACCGCAGGAGCCTCTGAAGCTCCCACCAGGCCAGCTC9820.10338278581866112No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC9750.10264584131689877No Hit
GATGTCCCTGATTGTCGAAGAACCATTTCGGCTCCTGTCGTTAGACCTAA9710.10222473017303457No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG9690.10201417460110247No Hit
GCGGACATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGG9660.10169834124320432No Hit
GCTACTTTGCTGCCATCCTTCACGGTGACACCCACGGTTACAGAGAGCAC9620.10127723009934012No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC9500.10001389666774753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGATA1150.044.0060045
GGGTAAG4600.044.0060041
TAAACGT1000.044.0060046
TTCGTCA502.7284841E-1144.0060045
ATATACT254.4386783E-544.0060045
TTACGAC254.4386783E-544.0060044
CCGGTCT4300.044.0060041
CGGTCCG254.4386783E-544.0060042
TATCGTG502.7284841E-1144.0060042
AAATGCG254.4386783E-544.0060044
TTGATTA454.783942E-1044.0066
TATCAAA207.8505167E-444.0065
CAACGAA551.8189894E-1244.0064
CCCTAAC207.8505167E-444.0061
TTTCGCG700.044.0066
TGGGTAA351.4441866E-744.0063
ACGGGTA207.8505167E-444.0066
TTTCATG351.4441866E-744.0066
ATCGTTG408.300049E-944.0066
TGCGCGG351.4441866E-744.0066
Produced by FastQC (version 0.11.5)