FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005292953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856008
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG328543.8380482425397893No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC197352.3054691077653477No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG169011.9743974355379854No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG113281.3233521182045027No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC110311.2886561807833572No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG83860.9796637414603602No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC81830.9559490098223381No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC80380.9390099157951795No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC76330.8916972738572537No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC73540.8591041205222382No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC71120.8308333566976009No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC66110.772305866300315No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG64030.7480070279717012No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG63550.7423996037420212No Hit
GCTGGCACAGGTGTGGACAATGTGGATCTGGAGGCCGCAACAAGGAAGGG62850.7342221100737376No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC60790.7101569144213605No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG54320.6345735086587976No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC49020.5726581994560799No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC46260.5404155101354193No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG43230.5050186446855637No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG33590.3924028747394884No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC30380.35490322520350276No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG29780.3478939449164027No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG29750.3475434809020476No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC29200.3411183073055392No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC29140.3404173792768292No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT27700.3235951065877889No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG27600.32242689320660556No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC27420.3203241091204755No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG26620.3109784020710087No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG26540.310043831366062No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA26410.30852515397052366No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC26080.3046700498126186No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC25970.3033850150933169No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG25270.2952075214250334No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG25080.29298791600078505No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG24540.28667956374239495No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA23620.2759320006355081No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC22910.2676376856291063No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC22580.2637825814712012No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG22580.2637825814712012No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC22500.2628480107662545No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG22440.2621470827375445No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT22300.2605115840038878No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC22180.2591097279464678No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA22070.2578246932271661No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG21610.2524509116737227No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG21470.250815412940066No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG21280.2485958075158176No Hit
GTGGCTCCAATACTTTGCCACCCATGAACTCAGGGTGTGCCCTGGGACAC20510.23960056448070577No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20470.23913327912823243No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC20450.23889963645199577No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC19950.233058569546079No Hit
CTAGTCAGACAAGTGACAGGGAATGGATTCCAAACACCAGTGTGTAAAGC19760.23083896412183064No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC19700.23013803609312064No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG18790.21950729432435212No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC18620.21752133157634043No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG18120.2116802646704236No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT18080.2112129793179503No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG17850.2085260885412286No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC17390.20315230698778516No Hit
GAGTCCCACCAGCCAGGCCTTATGAAAGTGATCATACTGGGCAGGCGTTG17250.20151680825412846No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG17250.20151680825412846No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG17210.20104952290165512No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG17060.1992972028298801No Hit
GAGTACACCTCATGTGGCAGAGCCTGCTGAGAATGAGCAGGATGAGCAGG16810.1963766693769217No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC16770.19590938402444835No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG16330.1907692451472416No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC16170.18890010373734825No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG16130.1884328183848749No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG16040.18738142634180988No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG15340.1792039326735264No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG15180.17733479126363305No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG15050.1758161138680947No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG14910.174180615134438No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA14840.17336286576760965No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC14820.17312922309137296No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC14480.16915729759534956No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC14480.16915729759534956No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG14230.1662367641423912No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG14200.16588630012803618No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC13850.16179755329389445No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC13820.16144708927953944No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG13620.15911066251717274No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC13440.15700787843104272No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG13320.15560602237362267No Hit
GCTCCACTCAGTGCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAGG13280.15513873702114933No Hit
GTGGACCTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATG13240.154671451668676No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG13010.15198456089195428No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG12910.15081634751077094No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG12900.1506995261726526No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT12620.1474285287053392No Hit
GGGATTTATCCGTCTGGGAGTGTGGTACAACTTCTTCCGAGCCTGGAACG12590.1470780646909842No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG12490.14590985130980083No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG12480.1457930299716825No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC12390.1447416379286175No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG12340.14415753123802583No Hit
GGTCATCCGGACATCCCAACGCATGCTCCTGGAGCTCACAGCCTTCTGTG12260.14322296053307912No Hit
CAACGTGGTCACCACGCTCGTGCTCATCTCCTACTGTCCCACGGCCACCG12060.14088653377071242No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG12010.14030242708012075No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG12000.14018560574200242No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG11950.13960149905141073No Hit
CGTCCAGAACTAGAAGCTGGTACAAATGGAAGAAGTTCGACAACAAGTCC11940.1394846777132924No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG11700.13668096559845236No Hit
GTGAAGACTTTGAACGCACTCCTCAGAGTCCGACTCTCCCACCTGCTAAG11380.1329426827786656No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC11370.13282586144054728No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG11270.13165764805936395No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC11270.13165764805936395No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT11070.12932122129699725No Hit
GCGATGAGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGAC11050.12908757862076053No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT10940.12780254390145887No Hit
CAACAGGGTCAGCTTCTGGAGGTTGGTTGGAACAATACGGCAAGTGCTCG10860.12686797319651216No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG10750.1255829384772105No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG10690.12488201044850047No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC10560.12336333305296213No Hit
CTATCGCCCTCACAAAGCCAATGCTGAGGAGATGACCAAGTACCACAGCG10530.12301286903860713No Hit
GCTAGCGAGCCAGAACATTGTGATCATCCTTTGTGGAAACAAGAAGGACC10410.1216110129811871No Hit
GACCAGTCGATGGCAGAATTCCACGGCAGTGGTCTAAAGCATTACCTTCT10280.12009233558564873No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG10200.11915776488070205No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG10180.11892412220446538No Hit
GTGTTTCTGAGCCCATCGACCTGACCGAGGGTGACTTTGCCAACGATGAG10150.11857365819011037No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC9990.11670451678021702No Hit
GAAGGTTTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCAAC9740.11378398332725861No Hit
GAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCTACTGC9630.11249894860795695No Hit
GCGGACATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGG9370.10946159381688021No Hit
GCAAGTGTTGCCAACCCATCTGATGGAAGAGCGTCTAATCCGACAGCAAC9280.1084102017738152No Hit
GGAAGCCGCCCGGCTCTTCAAGCAGATTGTCTCCGCCGTCGCCCACTGCC9270.10829338043569688No Hit
GTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGC9260.10817655909757852No Hit
CACGTCATTTGGTGGTAGTGGCATGGGCAACTTCAAATCGATATCAACTT9250.10805973775946018No Hit
CGAGTGTTGTCTCGACCCAATGCTCAGGAGCTTCCTAGCATGTACCAGCG9240.10794291642134185No Hit
GACTATTTGTCGGAGCACGGATCTACCACACCATTGCATATTTGACACCC9180.10724198839263184No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG9170.10712516705451351No Hit
TACTCTCACAGGTAGAATGAACTGTGTACTGGCCACATATGGAAGCATTG9170.10712516705451351No Hit
CTGCCTCAATCCTAGGTAACGAGGGCAACAGGGAGCACCTGTCTGGATTG9120.10654106036392184No Hit
GATGCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAA9090.10619059634956683No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC8900.10397099092531845No Hit
GCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAG8890.10385416958720012No Hit
CGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCG8800.10280277754413511No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG8770.1024523135297801No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT8760.10233549219166177No Hit
GGAGAGGGTAGCTGCCTGCATAGGAGCCTCGCTTCCGATTATTCCCTTCC8690.1015177428248334No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG8660.1011672788104784No Hit
CAAAGTCCGCCAAGGCACCCTGAAGAAGGCGCGCTACAATGCTCAGTGCC8650.10105045747236006No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC8590.10034952944365007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTAA302.5213958E-644.0140883
TAGCGCA302.5213958E-644.0140884
TCCGTCA302.5213958E-644.0140883
AATTATT302.5213958E-644.0140881
GGCATAT302.5213958E-644.0140886
CGGTCCG302.5213958E-644.0140882
CGATCAC302.5213958E-644.0140885
TATCATT454.783942E-1044.0140844
TTCTAAA2550.044.0140843
GTATTAC551.8189894E-1244.0140841
CGACGCA1050.044.0140841
CGTGAAC2350.044.0140845
GGGTAAG6550.044.0140841
GTTAGTT454.783942E-1044.0140846
GTCTATG650.044.0140841
CACGAAA1100.044.0140844
ATATACA1500.044.0140841
GAAACGT1900.044.0140842
GTCCGGA1050.044.0140842
GACGCGC750.044.0140845
Produced by FastQC (version 0.11.5)