FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005292964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005292964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069907
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC259832.4285288347491885No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG218802.04503755933927No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG143611.3422661969685217No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG108021.00962046233925No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC98370.919425707094168No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC90780.8484849617770516No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG80480.7522149121372231No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG57860.5407946672000463No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC55120.5151849646744998No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG53540.5004173259918853No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG53460.49966959745099343No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC52850.49396816732669285No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC52290.48873406754044973No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC51110.4777050715622947No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA48390.452282301171971No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC48050.4491044548731805No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG37210.34778723758233193No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC35630.3330195988997175No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC35390.3307764132770418No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC35240.32937442226286956No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG34520.3226448653948427No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT33400.31217666582235654No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG32930.3077837606446168No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG32810.30666216783327893No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG32760.3061948374952216No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC30440.2845107098093573No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC30050.2808655331725094No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG29230.2732013156283677No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG29040.2714254603437495No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG29020.2712385282085265No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC26630.24890013804938185No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG26560.24824587557610148No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG26130.24422683466880768No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG25960.24263791151941244No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG25030.23394556723154442No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG24720.23104811913558843No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG24030.22459896047039604No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC23920.22357083372666972No Hit
CACGCAGCTTCGGTTGCAAAGACTTTCTTGATGTCGGACTGCGTTGTTGT22870.21375689662746386No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA22050.2060926790833222No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG22020.20581228088048775No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG20820.19459635276710968No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20760.19403555636144076No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC20270.18945571904847802No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC18860.1762770035152588No Hit
CTTTCACTGCCACATCTTCTATTGGTTCAATGGTTGCTCCGAATGGTCAC18720.17496847856869804No Hit
CAAAGTAGACAAATGGCGCCGGGCACGGTGGCTCACGCCTGTAATCCCAG18640.17422075002780615No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC18120.16936051451200898No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT18000.16823892170067117No Hit
CCTCAGCACCTTCGAAGTGGTTCCTTGGCAGACTGCCTTTCCTGGAAGAG17700.16543493967232667No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC17650.16496760933426924No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG17500.163565618320097No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC17300.1616962969678673No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG16950.15842498460146537No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT16750.15655566324923567No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC16730.15636873111401273No Hit
CTTGCAGAGGTTAAAGCCAGCGGGTCCCTAGTCTTAGGCCCAGCCTGCTT16610.15524713830267492No Hit
GTGTGACTGTTGCAAGAGAGGTTGAGTTGGAAGACCCGGTTGAGAACATT16500.15421901155894857No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA16050.1500130385164318No Hit
GAGTTCCAGACCAGTGTTGTCTCCCGGGACATTGACACAGCAGCCAAGTT15800.1476763868261447No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG15690.14664826008241838No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC15540.14524626906824611No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT15420.1441246762569083No Hit
CACCCTCAGCTCTGGCCTCTTACTCACCCTCTACCACAGACATGGCTCAG15370.14365734591885088No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG15280.14281615131034753No Hit
GGGCTCTCCAAATAGGAGCCTCCCATCTCCAGGTGTTCTCTGTGGGTCTG14650.13692778905082403No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG14600.13646045871276663No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC14600.13646045871276663No Hit
GAAGTTCTGCGCTGGTCGGCGGAGTAGCAAGTGGCCATGGGGAGCCTCAG14360.13421727309009102No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG14130.1320675535350269No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG13830.12926357150668236No Hit
GGAGCAAGAGGTCAATTAGGAACTGGGCACACTTGTAATGTTAAGTGCCC13830.12926357150668236No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG13600.12711385195161823No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC13560.12673998768117228No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC13460.12580532700505745No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC13340.12468373419371964No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG13220.12356214138238185No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC13050.12197321823298661No Hit
GCTGGTGTAGCATCCGGTTCCTTTAGTGCAGCTAACTGCATTTGTCACTG12730.1189823040694191No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG12710.11879537193419615No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG12700.11870190586658466No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC12630.11804764339330427No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC12520.11701951664957796No Hit
GAAATACAGCCCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGAT12480.11664565237913202No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC12400.11589792383824013No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC12370.11561752563540569No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG12320.11515019529734828No Hit
CCCACTTGATTAGAGCAATGGGAAGCATACTGTGGCCTACCAGCATCTGG12240.11440246675645639No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG12080.11290700967467265No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC12050.11262661147183821No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGCTGTGACCTGCTGAG12030.11243967933661525No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC11990.1120658150661693No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG11640.10879450269976737No Hit
GGCTGCGGAGCTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGG11620.1086075705645444No Hit
GATGGAGACAGTATGCCGGCTCACCTTGGTGACCAGCCAAGATGTCTCAA11540.10785984202365254No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC11450.10701864741514916No Hit
GCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACGAGCC11370.1062709188742573No Hit
GGCCACTTTCCCTGTGTACACCATGGGCGCTATTCCAATTGTTTGCAAGG11320.10580358853619987No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC11320.10580358853619987No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT11270.10533625819814246No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG11200.10468199572486207No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG11150.10421466538680464No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTCAAGTATCGGGGTTGGCGGG11070.10346693684591278TruSeq Adapter, Index 3 (96% over 32bp)
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC10990.1027192083050209No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG10920.1020649458317405No Hit
CAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGACACCAG10720.10019562447951083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATTA1200.044.0213246
TATCATT650.044.0213244
GCGCGTC254.4299446E-544.0213244
GTCCTAT254.4299446E-544.0213245
TTCGTCA302.5191384E-644.0213245
ATCGTTG302.5191384E-644.0213246
TGCGCGT1000.044.0213245
TACGCAG254.4299446E-544.0213245
TCGTGAC302.5191384E-644.0213242
ACAATCT502.7284841E-1144.0213245
TGTTATA254.4299446E-544.0213246
ATGCGTT254.4299446E-544.0213246
ATGCGCG302.5191384E-644.0213245
TTACGCA254.4299446E-544.0213244
ACATAGC302.5191384E-644.0213244
TATGTAA254.4299446E-544.0213245
GTACGGA302.5191384E-644.0213245
CTATGCG700.044.021323
CGTATAT351.4404577E-744.021324
GGTAACA408.276402E-944.021321
Produced by FastQC (version 0.11.5)