FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141404
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG260182.279473350364989No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG114361.0019239462977174No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC65870.577096277917372No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG53330.467231584960277No Hit
GAAATACAGCCCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGAT49060.42982151806021357No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG43460.38075913524045824No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC42520.3725236638385707No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC35730.3130355246696174No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG34830.3051504988592996No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG32140.2815830328262386No Hit
GCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATG31720.2779033541147569No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG31640.27720246293161754No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG29360.25722706421214575No Hit
GAGTTCCAGACCAGTGTTGTCTCCCGGGACATTGACACAGCAGCCAAGTT28420.24899159281025823No Hit
CTCTGCACACTCCAGCCCACTTTCTGCCTTCAGCCATTGAGTGGAAGCTG27230.23856583646106022No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC27220.23847822506316785No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC25940.22726396613293806No Hit
GAATAACAGTAGTCCTTTGATAGAAGATAATGACTTGGTTTATGGCCTTA25910.22700113193926075No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG25540.22375951021724122No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC25250.22121877967836104No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC25000.21902849473105054No Hit
CAACCACTCCTGGAACACTCAGACCTGGTGCACCCAATCCTGGAACAATC24010.21035496633970094No Hit
GGTGAAAGGAGCCTGCCAGAAGACACTCAGCGACCTGAAGCTGGACTACC22030.19300790955700173No Hit
GGTCCATGGCAGACTTCTCTCGGCAGACACAAAGGGTTGGGTACGCCTGC21770.1907300132117988No Hit
CTGGGTGCCTATCTAGGTGGCATCATCTACCTGGTCTTCATTGGCTCCAC20980.1838087127782976No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC20570.18021664546470836No Hit
CACGTCATTTGGTGGTAGTGGCATGGGCAACTTCAAATCGATATCAACTT19860.17399623621434654No Hit
GATGAACGAAGCGTGCAAATCCACGTGGAACCTAAGTGCTCAGACCGAGG19220.16838910674923166No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG19130.16760060416819986No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG18910.16567315341456662No Hit
GTGAAGACTTTGAACGCACTCCTCAGAGTCCGACTCTCCCACCTGCTAAG18590.16286958868200918No Hit
CCAGCCAGATGAGTGCTCTGTGGACCCACAGCCTAAGCTGAGTGTGACCC18530.16234392029465464No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG17840.15629873384007767No Hit
GATGGACGGTAGCTTTGATTGTGATTGTGGTGAGCTGGAGCCACCTGATC17510.15340755770962777No Hit
GTGGGTTCAGGAATCCTCGTAGCCCACTACCTCCAGAGCATCACGCCTTG16870.1478004282445129No Hit
GCCACAGCTCTTGTGAGTACTGTCTCCCTCACGGTGAATGAGCCTCCTGG16860.1477128168466205No Hit
CCGCTCTCACAGCCATTGCAGTACATTGAGCTCCATAGAGACAGCGCCGG15860.13895167705737846No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG15550.13623572372271342No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG15380.13474632995854227No Hit
GAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCTACTGC15060.13194276522598483No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC14680.12861353210607288No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG14630.1281754751166108No Hit
GTTTCATCCTCTCCAGTGCGGCCAAGCACAGTGTCGATGGCGAATCCTTG14340.12563474457773058No Hit
GCTCCGCACTTGGCGGCGCAACCTGCGTGAGGCAGCGCGACTCTGGCGAC14240.12475863059880639No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT14230.12467101920091396No Hit
CTGTGTGCCTGCCAATCATGCTGCTCCTGCTACTGCCGTCGCTGCTGCTG14010.12274356844728072No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC13970.12239312285571105No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC13870.12151700887678683No Hit
GTCTACTGACCAATCAGTGTGGCATGAGGCCCACGCCACCCAAACCTTTC13820.12107895188732475No Hit
GGAGTCATCCGCAGAGTCACTCACCCACTGTGTTTCTGGTGCCAAGGCTC13770.12064089489786263No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC13420.11757449597162792No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG13340.11687360478848856No Hit
CAGCGTCAGATATCTGGAGACAGCCATACCCATGTTAGCAGACAGGGAGC12850.11258064629175997No Hit
CTCATTGCATGTCACCCTATGAATGTTGACAATGGAAGGAATACCTTGCC12670.11100364112969642No Hit
GGGCTCTCCAAATAGGAGCCTCCCATCTCCAGGTGTTCTCTGTGGGTCTG12590.11030274994655703No Hit
CCCTCTTTCATGTCCCACAGGCCACAGAGCCCAGTGTGTCCAACCAGCTG12470.109251413171848No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC12290.10767440800978445No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC12160.10653545983718299No Hit
GGCATGGGTGGCTACGTAAAGATGGCCAAAGACCGGAGAAACCATTGTGG12090.10592218005193604No Hit
GGAACAGTGTGGTTTGGGAAAGATGGATAAACTGAGCCTCACTTGCCCTC12020.10530890026668908No Hit
CAACATGAGACCAAAGTGTGTGACTAGTCAATGAAGTGCGACAGCATTAA11820.1035566723088407No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG11780.103206226717271No Hit
CAGCGCTGGTAGGGAGTCAGAAGGAGCCCAGCGAAGTGCCAACACCTAAG11680.1023301127383468No Hit
CGTTTGGTTAAAGCTTATTGCCATGCTGGTGCGGCTATGGAGACTGTCTG11670.1022425013404544No Hit
CCAGTCAGGTGTAGACTCTGGGATTGGAGAAAGTGTGCTTGTCTCCAGTG11580.10145399875942261No Hit
CCAACATAGCAGCTGTGATGTCATTTCTGTATTTCAGGAAGACTGGCAGG11570.10136638736153018No Hit
GATGCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAA11500.10075310757628325No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)