FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065645
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC291522.735620211233572No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG182791.7152991850006336No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG139761.3115061770101677No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC119141.118008342365422No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG105390.9889785059752545No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC101830.9555715083353276No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG99740.935958973204022No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC94570.887443754721319No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG90590.8500954820789287No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC78190.7337340296252505No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG76660.7193765278305627No Hit
TGTCGTGTCATACTCGAGTCTGAGGCCGTTGCTGACTTAGAATCCCTGTG74790.7018284700815No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC72670.6819344153071614No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG71570.671612028395948No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC64640.6065809908553036No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG63060.5917542896555608No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC60620.5688573586888692No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT56710.5321659652135561No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG54140.5080491157937211No Hit
CCCAGAGTTCACACCTTACCTGGAGACAAACCTCGGACAGCCAACAATTC50640.47520515743986036No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC49520.4646950907666249No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA44330.4159921925218999No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC44220.4149599538307786No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC43870.41167555799539246No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG42660.40032093239305777No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC40880.3836174335730942No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG40820.38305439428702803No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG40710.3820221555959067No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC40590.38089607702377437No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG40140.37667328237827796No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG39800.3734827264239029No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC39720.37273200737581463No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG37840.35509010974574085No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC36900.3462691609307039No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC36820.3455184418826157No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG36620.3436416442623951No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC35950.337354372234656No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC35420.33238085854107136No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG34970.328158063895575No Hit
CGCGAACGAGGCAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTG33950.31858639603244987No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG32720.307044090668093No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG31020.2910913108962178No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG30590.28705619601274346No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC29780.27945516565084993No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG28830.27054037695480204No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG28590.2682882198105373No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG27230.25552599599303705No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG27150.25477527694494884No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA27140.2546814370639378No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG26430.2480188055121546No Hit
CCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAGGCTTC25010.23469354240858825No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG24450.2294385090719705No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG24380.2287816299048933No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC24280.227843231094783No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT24170.22681099240366162No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG24100.2261541132365844No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG23930.2245588352593969No Hit
GTGTTTCTGAGCCCATCGACCTGACCGAGGGTGACTTTGCCAACGATGAG23710.22249435787715421No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG22810.21404876858616145No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC22510.21123357215583052No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT22050.2069169376293231No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC21930.20579085905719072No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG21690.20353870191292597No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG21490.20166190429270536No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC21460.20138038464967228No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT21340.20025430607753988No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC21070.19772062929024206No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC21040.19743910964720895No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC20220.18974423940430443No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG19700.1848645655917308No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC19580.18373848701959847No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG19510.18308160785252126No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC19290.18101713047027856No Hit
GTTGATGCCAGACATCACCAGGTTGTAGAAGTTGACAGGCAGTGCCATGG19250.18064177094623443No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG19090.17914033285005793No Hit
GGACCAGTCGTTGTTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGA18920.17754505487287042No Hit
GACCAGTCGATGGCAGAATTCCACGGCAGTGGTCTAAAGCATTACCTTCT18770.17613745665770497No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC18760.17604361677669392No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC18530.1738852995134402No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA18340.17210234177423064No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG18220.17097626320209824No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG18200.1707885834400762No Hit
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC18130.17013170427299898No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG17630.16543971022244744No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC17620.1653458703414364No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG17510.16431363165031507No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC17440.16365675248323786No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG17320.16253067391110548No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC17060.1600908370048187No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG16990.15943395783774147No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC16950.15905859831369734No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG16740.15708796081246568No Hit
CTGGCTTGACCACTGGATGCTTTGCCGAAAGCAGCTTGGCTTGGTAGCTC16690.15661876140741052No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG16550.15530500307325612No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG16230.1523021268809031No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG16210.15211444711888106No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG16100.1510822084277597No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG16060.15070684890371558No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG15990.15004996973663837No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG15920.14939309056956115No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG15810.14836085187843984No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC15770.14798549235439568No Hit
GAAGTTCTGCGCTGGTCGGCGGAGTAGCAAGTGGCCATGGGGAGCCTCAG15710.14742245306832952No Hit
CTGTTGTGTGGTGGCGCTCCACTTTCTGCAACCACGCAGCGATTCATGAA15510.1455456554481089No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG15480.1452641358050758No Hit
GAAGCCTAGGAAAGACCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGG15460.14507645604305372No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG15280.1433873381848552No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC14810.13897686377733673No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT14810.13897686377733673No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG14720.13813230484823744No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG14620.13719390603812714No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG14520.13625550722801683No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG14420.13531710841790653No Hit
GCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACGAGCC14230.13353415067869695No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC14080.1321265524635315No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG14030.13165735305847634No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT13930.130718954248366No Hit
GCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAG13790.1294051959142116No Hit
GTAAGAAATTGTTTATATGTTGCATTTGTACTTAAGTCATTCCATCTTTC13380.12555776079275932No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG13320.12499472150669312No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC13250.12433784233961591No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG13180.1236809631725387No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG13170.12358712329152766No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG13130.12321176376748355No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG12910.12114728638524086No Hit
ATGGCTTCCAGAGGCCGACGATCTACTATGTGATGTCAGGGCCTGCGTGG12870.12077192686119674No Hit
CTATCGCCCTCACAAAGCCAATGCTGAGGAGATGACCAAGTACCACAGCG12850.12058424709917469No Hit
GCGATGAGGAGCATGATCAGGAAGGCCGGGTGATTGTGGCTGAATTTGAC12850.12058424709917469No Hit
CGAGTGTTGTCTCGACCCAATGCTCAGGAGCTTCCTAGCATGTACCAGCG12650.11870744947895406No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG12630.118519769716932No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG12430.11664297209671137No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA12400.11636145245367828No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG12270.11514153400053488No Hit
GAGCAAGCAGCGAGTCTTGAAGCTCTGTTTGGTGCTTTGGATCCATTTCC12250.11495385423851281No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC12160.11410929530941355No Hit
CTCAGCTCTTGCCAGGCCAAATTGAGACATGTCTGACACAAGCGAGAGTG12120.11373393578536942No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT12110.1136400959043584No Hit
GCAGTTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCG12090.11345241614233632No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG12080.1133585762613253No Hit
CATCTTGCCTGGAGCAAGGATATCAATGCTTACAATTGTGAAGAGCCCAC11930.11195097804615985No Hit
GCGGACATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGG11890.11157561852211571No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG11880.11148177864110469No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG11810.11082489947402746No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG11660.109417301258862No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC11630.1091357816158289No Hit
GTGGGAACGTCTTACGGTAGCTGATGCATTGGAACCAGTGCAGTTTGAAG11590.10876042209178478No Hit
GCTAGCGAGCCAGAACATTGTGATCATCCTTTGTGGAAACAAGAAGGACC11470.10763434351965243No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC11430.10725898399560829No Hit
GTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTG11280.10585138578044283No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC11190.10500682685134356No Hit
GTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCG11170.1048191470893215No Hit
GGCTGCGGAGCTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGG11150.10463146732729942No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG11020.10341154887415602No Hit
CTCCTCACAGCCCACTGGTCCTCAAGAGGTGCCACGTCTCCACACATCAG10980.10303618935011191No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG10680.10022099291978098No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG10670.10012715303876994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAC351.4442594E-744.0065732
CGTATAT254.4387067E-544.0065734
GCGAAAC207.850446E-444.0065734
ACCGCGT207.850446E-444.0065733
CGTGATA650.044.0065735
TTGCTAA207.850446E-444.0065736
GTCCTAT502.7284841E-1144.0065735
CGTGAAT254.4387067E-544.0065735
CGTGAAC1000.044.0065735
TGGGTAA302.5249465E-644.0065733
TAGCGCA408.300049E-944.0065734
GGTCCGT302.5249465E-644.0065733
CGCGCGA207.850446E-444.0065731
TTCGTCA302.5249465E-644.0065735
CACGAAA600.044.0065734
TGACGGC254.4387067E-544.0065736
ACGGGTA207.850446E-444.0065736
AATATTA502.7284841E-1144.0065736
TACGCGT408.300049E-944.0065735
ATATACC351.4442594E-744.0065733
Produced by FastQC (version 0.11.5)