FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974206
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGCCGTC611926.281217730130999TruSeq Adapter, Index 6 (97% over 34bp)
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC533225.473380373350195No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG164761.6912234168132818No Hit
CACGCAGCTTCGGTTGCAAAGACTTTCTTGATGTCGGACTGCGTTGTTGT138881.4255711830967988No Hit
CTTTCACTGCCACATCTTCTATTGGTTCAATGGTTGCTCCGAATGGTCAC112651.1563262800680758No Hit
GTGTGACTGTTGCAAGAGAGGTTGAGTTGGAAGACCCGGTTGAGAACATT102431.0514203361506704No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG92760.9521600154382132No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGC66640.6840442370504801TruSeq Adapter, Index 6 (97% over 38bp)
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC66030.6777827276777191No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC65230.6695709121068849No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG64980.6670047197409993No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC57740.5926877888249508No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG52610.5400295214769771No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGCTGTGACCTGCTGAG49540.5085166792239013No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGCC42120.43235208980441514TruSeq Adapter, Index 6 (97% over 37bp)
GATGGAGACAGTATGCCGGCTCACCTTGGTGACCAGCCAAGATGTCTCAA40070.4113093124041527No Hit
GCTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCG39190.4022763152762352No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG36320.37281642691586786No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC33780.34674391247846964No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT33750.3464359693945634No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC28960.2972677236641942No Hit
GCAAGGAGAGTCCCACGCCATTCACATGTCCTGGTGGACTCTCTTGGCCG28900.29665183749638163No Hit
CACGCAGCTTCGGTTGCAAGACTTTCTTGATGTCGGACTGCGTTGTTGTG28820.29583065593929825No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC27590.28320498949914086No Hit
GAATAAGCTAGCCCAGCCACACCACCTTGTTGTGTGACCTTGGGCAGGTG27500.282281160247422No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGTGTGACCTGCTGAGG26980.2769434801263798No Hit
GTCAGAGTTGTGCCACCGTCCAGCGGCTGCAGCACACACTCCAACAGGTG25000.25661923658856545No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATGTCGTATGCCGTC24780.2543609873065861TruSeq Adapter, Index 6 (97% over 34bp)
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG23850.24481475170549144No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGCCG22970.23578175457757397TruSeq Adapter, Index 6 (97% over 36bp)
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG22680.2328049714331466No Hit
CGAGTGCGAGTACTACCCGGTGGTGCCTGGGAAGTATGTGGTGACCATCA21690.2226428496642394No Hit
GCAAGAGAGTCCCACGCCATTCACATGTCCTGGTGGACTCTCTTGGCCGA21440.2200766572983537No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG21350.21915282804663488No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG20840.21391779562022817No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC20120.20652716160647747No Hit
CTGTGTGCCTGCCAATCATGCTGCTCCTGCTACTGCCGTCGCTGCTGCTG19910.2043715600191335No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG18650.19143795049506984No Hit
AGCCGACCTGCCTACAGACCCGCCTGGGCTGTACAAGCAGGGCCTGCGGG18280.18763998579355906No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG18250.18733204270965279No Hit
CGAGGGTCCGCTTCGTCTTTCGAGAGTGACTCCCGCGGTCCCAAGGCTTT17200.17655403477293302No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC17130.17583550091048505No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG16970.17419313779631823No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG16870.173166660849964No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG16740.17183224081970344No Hit
GCGTGGCATTGGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCG16700.17142165004116172No Hit
GCTGGCATTGGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCGA16630.17070311617871375No Hit
CCTTCTGCGAGGATATGGCCGCCCAGGTGGGCGTGAGCATCCAGGCAGTG16410.16844486689673435No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG16290.16721309456110925No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC16090.16516014066840073No Hit
CCGTGGTGTACGTGCAGAGCGCGCAGAGCGAGTGGCGCCCGTATGCCCTG16070.16495484527912987No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT15750.16167011905079623No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATATCGTATGCCGTC15230.1563324389297541TruSeq Adapter, Index 6 (97% over 34bp)
GCAAGGAGAGTCCCACGCCATTCACATGTCCTGGTGGACTCTCTTGCCGA14920.15315036039605587No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG14720.15109740650334735No Hit
GCGTGGCATTTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCG14610.14996828186235764No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC14210.1458623740769406No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG13690.14052469395589845No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG13310.13662408155975225No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAAGCTCGTATGCCGTC12660.12995198140844955TruSeq Adapter, Index 6 (97% over 34bp)
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG12540.12872020907282442No Hit
GGATCTCAGGCGTGGTCCGAGAGGGACGGCGTGAGAACCAGCTAAAGCTG12470.12800167521037645No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA12310.12635931209620963No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC11940.12256134739469886No Hit
GCGTGGCATTGTGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCG11920.12235605200542801No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG11630.11937926886100064No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCGGTGGACTCTCTTGGCCG11630.11937926886100064No Hit
CTGATCCTGTAACGCTGCTGGAGGTCAGTCTGAGCTACCAAGACTGTCCC11600.11907132577709437No Hit
CTGCTGCGCGTGCCAGGCGCTTCTCTGGGCTGTCTGCTGAGCTCCGGAAC11560.11866073499855266No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC11490.1179422011361047No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG11420.11722366727365671No Hit
CATGCTCGCCATGCTGCCGCTTATCCCGGTGGGATAGATCGGTTCGTGTC11290.11588924724339617No Hit
ACGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC11260.11558130415948989No Hit
GGAGTGGAGAGGCATTCCCAGAAGCATCAGCTTCGGGCATCTCCAGAACC11050.11342570257214593No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC10950.11239922562579166No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG10870.11157804406870825No Hit
GTACGACGCGCACACCAAGGAGATCGACCTGGTCAACCGCGACCCTAAAC10820.11106480559553113No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG10450.10726684089402037No Hit
CTCCTGTGGTCTCAGGCTTGCGCTGGACCTGGTTCTTAGCCCTTGGGCAC10180.10449535313886385No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG10160.104290057749593No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC10130.10398211466568673No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC10020.10285299002469703No Hit
CAGACCTCCCTACAGCTGTAGGAACTACTGTCCCAGAGCTGAGGCAAGGG9940.10203180846761362No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCGGTGGACTCTCTTGCCGA9910.10172386538370734No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC9810.10069738843735308No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATTA207.8349235E-444.0238846
ATTAGCG408.270945E-944.0238846
AATATTA207.8349235E-444.0238846
TACATTT302.5178979E-644.0238845
GTTAAAT207.8349235E-444.0238845
AACGAGT207.8349235E-444.0238846
GTATATG207.8349235E-444.0238845
CGGCTTA302.5178979E-644.0238846
TTAGTCC408.270945E-944.0238845
ATGCGCG850.044.0238845
TAGTCGG408.270945E-944.0238845
AGTCGAT207.8349235E-444.0238845
AGCAATT351.439621E-744.023885
GGTAACT454.765752E-1044.023885
TTTCGCG254.4281303E-544.023886
GTTCGGT254.4281303E-544.023886
AAATTAC2700.044.023885
TCAAGGG254.4281303E-544.023885
CTTACAT351.439621E-744.023886
CGTATAT207.8369054E-444.021624
Produced by FastQC (version 0.11.5)