FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030443
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC729887.083167142675528No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG236512.2952264220340184No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG99230.9629838816897199No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA97000.9413427040602926No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC93820.91048219066945No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC80970.7857785437913597No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC74610.7240575170096745No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG68180.6616571707508324No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG64950.6303114291620205No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC63940.6205098195630423No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC63900.6201216370046669No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC63470.6159486745021316No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG57800.5609237968524217No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC57600.5589828840605447No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC54360.5275400968321392No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC53070.5150212093245332No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT49210.47756159244130925No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC47560.4615490619083249No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC47280.4588317839996972No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC46900.4551440496951311No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC45100.4376758345682391No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG43440.4215662583956609No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA42650.41389965286774716No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC41760.405262590943895No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG40340.3914821101215691No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG39710.3853682348271569No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG39070.3791573138931508No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG37490.36382410283732336No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA34260.33247836124851154No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG33090.32112402141603175No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC31530.305984901639392No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG30640.29734783971553985No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG30400.2950187443652876No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC30310.29414533360894296No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC29870.28987532546681377No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG29020.281626446101337No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG29000.2814323548221493No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG28950.2809471266241801No Hit
GCTTCTCCAGCTTTGTGCCTGTCACTATTCCTCATGCCACCACTGCCAAC28940.28085008098458625No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG27900.27075733446682637No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG27810.2698839237104818No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG27520.26706960016226033No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC27430.2661961894059157No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG26720.25930594899475273No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG26580.2579473100404389No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG25510.24756342660389755No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG25490.24736933532470987No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG25360.2461077420099899No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG25230.24484614869526988No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC25030.242905235903393No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC24960.24222591642623612No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG24840.24106136875110995No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG24550.23824704520288847No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG24470.2374706800861377No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC24350.23630613241101156No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC23640.2294158919998486No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG23260.2257281576952825No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC22730.22058473879680876No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG21700.21058903791864275No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG20990.2036987975074798No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC20690.20078742831966448No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC20460.19855537860900604No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC20300.19700264837550452No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC19760.1917621838374369No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG19430.18855967773084004No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC18880.18322216755317858No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC18660.181087163482114No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC18590.1804078440049571No Hit
CTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGG18500.17953443324861248No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG18470.17924329632983096No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG18340.177981703015111No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG18270.17730238353795405No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG18100.1756526076648587No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC17940.1740998774313572No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA17930.17400283179176335No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG17870.17342055795420028No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC17450.16934464109125882No Hit
AGCTTTACTACCAGAGTTACGAAGAGCATCAAATCTTCTTTATGGTCAGC17450.16934464109125882No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG17420.16905350417247728No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG17170.16662736318263116No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG17020.1651716785887235No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG16910.1641041765531912No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT16820.1632307657968466No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC16780.1628425832384712No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC16760.16264849195928355No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG16470.15983416841106204No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG16390.1590578032943113No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC16150.15672870794405902No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG16060.1558552971877144No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG16000.15527302335015133No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC15670.15207051724355447No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG15620.15158528904558524No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC15390.1493532393349268No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC15350.14896505677655145No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG15300.14847982857858222No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG15230.1478005091014253No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG15210.1476064178222376No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG15170.14721823526386224No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC15130.14683005270548685No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG14930.14488913991360997No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG14920.14479209427401613No Hit
TCAGCTAAAGCCCAACCTCCTGACCTGTCACCGTGAGCAGCTTTGGAAAC14750.14314231840092076No Hit
GTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTG14680.14246299892376385No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT14580.1414925425278254No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG14570.14139549688823155No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG14570.14139549688823155No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC14460.14032799485269928No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG14340.13916344717757315No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC14300.13877526461919776No Hit
GACTATTTGTCGGAGCACGGATCTACCACACCATTGCATATTTGACACCC14120.13702844310650855No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG13990.13576684979178857No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG13940.13528162159381935No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG13790.1338259369999117No Hit
GCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTG13580.13178797856844096No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG13550.13149684164965944No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG13470.13072047653290866No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG13400.13004115705575175No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG13270.12877956374103178No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC13040.12654751403037334No Hit
CCCACTTGATTAGAGCAATGGGAAGCATACTGTGGCCTACCAGCATCTGG12970.12586819455321643No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC12830.12450955559890262No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC12740.12363614484255801No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG12710.12334500792377646No Hit
GCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACGAGCC12580.12208341460905649No Hit
CCGCAGGATATACGCCAACATGTTCAAGAAGTTTGCAGAGCAGGATGCCA12340.11975431925880423No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG12160.11800749774611502No Hit
CAACAGGGTCAGCTTCTGGAGGTTGGTTGGAACAATACGGCAAGTGCTCG12140.11781340646692734No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG11990.11635772187301965No Hit
CTTGGGCCGACCACTCTGTTCTCATTCTCCTTCCCACCAACATCCATCCG11820.1147079459999243No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC11810.11461090036033048No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG11730.1138345352435797No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT11710.11364044396439202No Hit
GGGCTTGTCCTCCTTTCTGTTACGGTCCAGGGCAAGGTCTTTGAAAGGTG11710.11364044396439202No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT11660.11315521576642279No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG11630.11286407884764126No Hit
GAAGGTTTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCAAC11570.11228180501007819No Hit
GCTGACATACAAATGCCATTCACCTGCTCGGTGACATATTATGGACCATC11550.1120877137308905No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG11370.1103408922182013No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT11350.1101468009390136No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG11300.10966157274104439No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG11190.1085940707055121No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG11080.10752656866997981No Hit
CATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGCGCT11070.10742952303038596No Hit
CGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCG11000.10675020355322906No Hit
GCGGACATGGCCTCAAACGATTATACCCAACAAGCAACCCAAAGCTATGG10990.10665315791363521No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC10970.10645906663444751No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG10840.10519747331972752No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT10820.10500338204053984No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC10810.104906336400946No Hit
GACACAACTGTGGGCTTTCTGAGGCGATGCCCGTTGGGCAAACTTAGGTT10660.10345065180703834No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA10640.10325656052785064No Hit
GGTAGCCGGACTCAGCTGCGAGGAGATGGGCTTCCTCAGGGCACTGACCC10570.10257724105069374No Hit
CTTTCTTCTGCCCTTTCCTAGGCCCAGGCCTGGGTTTGTACTTCCACCTC10490.10180087593394298No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT10470.10160678465475528No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT10410.10102451081719223No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG10310.10005405442125377No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)