FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099308
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAAGCTAGCCCAGCCACACCACCTTGTTGTGTGACCTTGGGCAGGTG11508010.46840375945595No Hit
CGAGGGTCCGCTTCGTCTTTCGAGAGTGACTCCCGCGGTCCCAAGGCTTT460784.1915459543640186No Hit
CCGACCTCGTGGCGCTGTGCAAGAAACGCCTCAAGCGCCACGGCAAGTAC403523.670672823267001No Hit
CATCAAGGATCAGGACCGCAAGTCCCGTCTTATGGGACTTGAGGCCCTCA261762.381134313586365No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC137741.2529700502497936No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCTGGTGGACTCTCTTGGCC118161.0748580015791753No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC111921.0180950197760772No Hit
GAGAAGGACACTCGCTGCCTGGGTGCGACTGCACAGCAGTTCCACAGGCA83100.7559300942047179No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC76080.6920717396762327No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG62780.571086538076681No Hit
CTGTCCCGAGCTAGGTGACAGCGTACCACGCTGCCACCATGAATGAGGTG48470.4409137384609227No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC45680.41553413602011446No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG45350.4125322475593737No Hit
CGGGAACACAACAGAGTATGCGATGTGCTTAAACAGGAGCATCCTGAATG44810.40762006644179793No Hit
CAAAGTAGACAAATGGCGCCGGGCACGGTGGCTCACGCCTGTAATCCCAG43650.39706797367070923No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT40340.3669581227463095No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCTGTGGACTCTCTTGGCCG38690.3519486804426057No Hit
CATCAAGGATCAGGACCGCAAGTCCCGTCTTAGGGACTTGAGGCCCTCAG38510.3503112867367471No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG38160.34712746564202207No Hit
CCAGTAGAGTTGGAGCAAGTGCGCCAGAAGCTGCTGCAGCTGCTCCGCAC37630.34230625084143845No Hit
GGTGAAAGGAGCCTGCCAGAAGACACTCAGCGACCTGAAGCTGGACTACC37200.33839469921077625No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC36160.3289342022435932No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA34780.31638085049867737No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG34190.31101383779614084No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG33970.3090125788223137No Hit
GAGATGCCTGATGGTTCAAGTAGAGGCCCAGTACATCTTGATCCATCAGG33960.3089216125053215No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC33900.30837581460336866No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG33400.30382749875376147No Hit
GCAAGGAGAGTCCCACGCCATTCACATGTCCTGGTGGACTCTCTTGGCCG33120.3012804418779814No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC32250.2933663722996649No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC31730.2886361238160734No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCGGTGGACTCTCTTGGCCG30550.2779020984110004No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC28240.2568888791858151No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC27870.25352312545710576No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG27830.2531592601891372No Hit
CGCGCCCGAAGCGCCTGTCTACGCGAACCTGAGCAGCTACGCGGGCGGCG27490.2500664054114043No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG27010.24570002219578135No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC26870.24442649375789133No Hit
CAGACCTCCCTCAGCTGTAGGAACTACTGTCCCAGAGCTGAGGCAAGGGG26660.2425162011010563No Hit
CTGCGAGGTTAACAATGAGGACTTACTCTGTGTCCTCATTGATGATGGAG26420.24033300949324485No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG26360.23978721159129196No Hit
CCGGCAGGGCTGTGAGCCCATACTCATCAAGTACCGCCACTCGTGGCCGG26300.2392414136893391No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG26290.23915044737234697No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC25830.23496599679070834No Hit
GTCAGAGTTGTGCCACCGTCCAGCGGCTGCAGCACACACTCCAACAGGTG25690.23369246835281832No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT25160.2288712535522347No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG25130.22859835460125824No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG24960.22705192721239179No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA24640.22414100506864318No Hit
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC23960.2179552955131774No Hit
CAGCTTCATCTGTCTCCAAGGTTAACATTCTGAAGCTGGAGAGTCCTTGG23670.21531727232040518No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC22990.2091315627649394No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG22730.20676643852314364No Hit
CCAGGACGGAGACCTCACCCTGTACCAGTCCAATACCATCCTGCGTCACC22690.20640257325517508No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGCTGTGACCTGCTGAG22240.20230908899052857No Hit
GTCTCGGATGGATAAGGTGGAACGCCTGAAACAGGTGTTGCTCCACCAAC22120.20121749318662285No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC21610.19657821102002349No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG20670.18802737722276194No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC20540.18684481510186407No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC20130.18311519610518617No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGTGTGACCTGCTGAGG20130.18311519610518617No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG20110.18293326347120187No Hit
CGACACGGTGAAGCGGTTGTTAGCAGAAGGTATCAACCGCCCGCGGAAAG19800.18011330764444541No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC19600.17829398130460253No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCTGGTGACTCTCTTGGCCG19440.17683852023272822No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC19270.17529209284386177No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC19250.1751101602098775No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG18800.171016675945231No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG18100.1646490337557809No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG18050.16419420217082017No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG17990.16364840426886731No Hit
TGCTGAGAGTGCGGAGTGTGTGCTCCGGGCTCGGAACACACATTTATTAT17880.16264777478195372No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA17630.16037361685715013No Hit
CGAGGAGTAGAGAGGCAGAAGAGATGTCAGCAAAGCAAGTGATGAAGCAG17470.15891815578527582No Hit
CTTCAGCCGCCTCCTTGACAGTCTTCGGCAGGAAGGGTTCCTCACAGATG17200.15646206522648792No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG17180.15628013259250365No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC16980.15446080625266076No Hit
GCAAGAGAGTCCCACGCTCATTCACATGTCCTGGTGGACTCTCTTGGCCG16390.14909379355012425No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC16170.1470925345762971No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG16040.14590997245539922No Hit
TTCCGGCCCGGCCACGAGTATTACTACATCTCTGCCACGCCTCCCAATGC16010.1456370735044228No Hit
CCGCAGGATATACGCCAACATGTTCAAGAAGTTTGCAGAGCAGGATGCCA15990.14545514087043848No Hit
GATGGAGACAGTATGCCGGCTCACCTTGGTGACCAGCCAAGATGTCTCAA15820.14390871348157203No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC15600.14190745450774486No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG15530.14127069028879988No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG15460.14063392606985486No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG15440.1404519934358706No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG14940.13590367758626334No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC14930.13581271126927122No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG14710.13381145229544406No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG14650.1332656543934912No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG14600.13281082280853046No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC14410.1310824627856797No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG14380.13080956383470327No Hit
GCAAGGAGAGTCCCACGCTCATTCACATGTCCTGGTGGACTCTCTTGCCG14360.130627631200719No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG14300.13008183329876613No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG14270.1298089343477897No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG14120.12844443959290755No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGCTGTGACTGCTGAGG14040.12771670905697038No Hit
CTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAGCTGC13860.1260793153511118No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG13730.12489675323021392No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC13600.12371419110931606No Hit
CCAGACCATGGACAACCATATGCAGCTGGGCATGATCTGCTACAATCCAG13350.12144003318451244No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC13180.11989360579564598No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC13110.11925684157670098No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC12600.11461755941010164No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC12600.11461755941010164No Hit
GCCTTTGGCACTACAAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTC12410.1128891993872509No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG12300.1118885699003373No Hit
AACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGG12230.1112518056813923No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG12180.11079697409643158No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC12080.10988731092651013No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC12070.10979634460951798No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC12040.10952344565854155No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA12030.10943247934154941No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG12030.10943247934154941No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC11950.10870474880561225No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG11870.10797701826967511No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG11830.10761315300170653No Hit
CTGAACGATGGTCGCTGCATTGACCTGGTCAATGGCTACCAGTGCAACTG11800.1073402540507301No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG11730.10670348983178508No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT11570.1052480287599108No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC11430.10397450032202077No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG11330.10306483715209931No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG11310.10288290451811502No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG11200.10188227503120145No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC11140.10133647712924856No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC11110.10106357817827215No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT11020.10024488132534286No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC11010.1001539150083507No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG11000.10006294869135857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)