FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711699
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG500762.925514357372412No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG414552.42186272235948No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC320471.8722333774805033No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC276521.6154709443658026No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC236581.3821355273327844No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC174171.017527030161261No Hit
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC153560.8971203465095205No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG137650.8041717615071341No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC135350.7907348196148972No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG129150.7545134979923456No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG123920.7239590605591287No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG116300.6794418878552829No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG112250.6557811858276484No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC98970.5781974517716023No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG98370.5746921625823233No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT98310.5743416336633953No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG91770.536133981500252No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC90190.5269033866351502No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA78960.46129605730914136No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG71290.41648677717285576No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG68700.40135561217246724No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT66380.3878018273072544No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC66200.38675024055047064No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT66190.38669181906398264No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC63360.37015853838788243No Hit
AACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGG61280.35800686919838126No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT60700.354618422982078No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC60370.35269051392797446No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC60050.35082102636035895No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC60040.35076260487387095No Hit
CTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAGCTGC58810.34357676203584864No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG58690.34287570419799274No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG57650.33679986960324215No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG56660.33101614244093147No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG54690.31950710960279816No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG51860.302973828926698No Hit
GGAAGGATTTCGCTGCTTCCCGAAGGTCTTGGACGAGCGCTCTAGCTCTG51310.29976064716985873No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG48180.281474721899119No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG47810.27931312689906346No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG46900.27399677162865665No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG46260.2702577964934255No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG45540.2660514494662905No Hit
CTGTGAATCACTGCAGGCTGTGTTCTTGCATCTTGTCTGCAAACAGGTCC44790.2616698379796915No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG44490.2599171933850519No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG44300.25880718514178014No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC43610.2547761025741091No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG42610.24893395392531045No Hit
CACGCAGCTTCGGTTGCAAAGACTTTCTTGATGTCGGACTGCGTTGTTGT42340.24735657379013481No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG41690.2435591771684157No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC40710.23783387149259302No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG39050.22813590473558729No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC37600.21966478919482924No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG37500.21908057432994937No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG37130.21691897932989385No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG36510.2132968471676387No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG36420.21277105378924682No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG36390.21259578932978285No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC36160.21125209514055915No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT35700.20856470676211178No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC35650.20827259932967185No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC35650.20827259932967185No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG35630.20815575635669592No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG34780.20318993000521704No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC34690.20266413662682514No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT34440.2012035994646255No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC34310.20044412014028168No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC34290.2003272771673057No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC33470.1955367152752908No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC32990.19273248392386746No Hit
CTTTCACTGCCACATCTTCTATTGGTTCAATGGTTGCTCCGAATGGTCAC32810.1916808971670837No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA32470.18969456662649217No Hit
GTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTG32400.18928561622107626No Hit
TGTCGTGTCATACTCGAGTCTGAGGCCGTTGCTGACTTAGAATCCCTGTG31950.18665664932911685No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC31270.18268398824793378No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG31160.18204135189656592No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG30990.18104818662627017No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC30220.1765497321666952No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG29610.172986021490928No Hit
GTGTGACTGTTGCAAGAGAGGTTGAGTTGGAAGACCCGGTTGAGAACATT29580.17281075703146406No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC29140.17024021162599265No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG28670.1674944017610573No Hit
GGACCAGTCGTTGTTTGAGTGTGCCTACGGAACGCCACACCTGGCTAAGA28430.16609228608534563No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC28370.1657417571664177No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC28070.1639891125717781No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG27970.16340489770689823No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG27630.1614185671663067No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG27090.15826380689595543No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC26950.15744590608512363No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG26880.1570369556797077No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG26570.15522588959858014No Hit
GCTCCACTCAGTGCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAGG26350.1539406168958444No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC26140.1527137656795967No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG26100.15248007973364475No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG25840.15096112108495713No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG24460.142898955949615No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG24440.142782112976639No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG24260.14173052621985524No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG23980.14009472459819164No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG23810.13910155932789586No Hit
GAAGGAACTCCTGTTGCACATCCGAAGTCATAAACAGAAGGCTTCTGGCC23800.13904313784140787No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG23330.1362973279764725No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC23290.13606364203052054No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC23220.13565469162510466No Hit
CACCAAGTGCTCCATAAGCAAGTTGGAACTCAGCGCAGCCGAACAGGAAC22950.13407731148992902No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC22870.13360993959802514No Hit
CTGGAGAGTCCAGGAGAGAAGACTCACCTCTGTCGCTTGGGTTAAACAAG22660.1323830883817774No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG22630.13220782392231345No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC22590.1319741379763615No Hit
CTGGCTTGACCACTGGATGCTTTGCCGAAAGCAGCTTGGCTTGGTAGCTC22410.13092255121957774No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC22320.13039675784118587No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT22100.12911148513845017No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG22070.1289362206789862No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG22030.12870253473303425No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC21710.12683304716541868No Hit
TGGAAGAGGTCCAGGATGGCAAGGTCATCTCCTCCCGCGAGCAGGTCCAC21650.12648251824649076No Hit
CCCACTTGATTAGAGCAATGGGAAGCATACTGTGGCCTACCAGCATCTGG21630.1263656752735148No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC21570.12601514635458688No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC21440.12525566703024305No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG21290.12437934473292324No Hit
GTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCG21180.1237367083815554No Hit
AGAGCCAACATGTTGGTATTGCTGGCTGGTATCTTTGTGGTCCACATCGC21060.12303565054369955No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG20970.12250985716530768No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG20900.12210090675989178No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC20820.12163353486798788No Hit
CGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCG20810.1215751133814999No Hit
CCGCAGCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTG20320.11871246054358855No Hit
GCCATAGTCGTGCCTGTTTGCTTAGCATTCCTATTGACAACTCTTCTGGG20290.11853719608412461No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG20200.11801140270573272No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG20180.11789455973275674No Hit
TACTCTCACAGGTAGAATGAACTGTGTACTGGCCACATATGGAAGCATTG20150.1177192952732928No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC19680.11497348540835742No Hit
CATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGCGCT19060.11135135324610225No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG18870.11024134500283052No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG18780.10971555162443865No Hit
GGAGATTGGTCACATGTTCAGTTGTCTGAGATACTGGTAAATGTGCGCTG18770.10965713013795066No Hit
AGTTGAAATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATAGC18560.10843027892170296No Hit
CGAGGGTCCGCTTCGTCTTTCGAGAGTGACTCCCGCGGTCCCAAGGCTTT18510.10813817148926301No Hit
GGTTCCCGTTTCTCCACCTAGACTGTAAACCTCTCGAGGGCAGGGACCAC18450.10778764257033507No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG18430.10767079959735912No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC18330.10708658473247924No Hit
GTTTGGATACCCTTAGTGGGATGATGTAAATAGAGGCTAGCTACCTAGGC18110.10580131202974355No Hit
GATGTCCCTGATTGTCGAAGAACCATTTCGGCTCCTGTCGTTAGACCTAA17850.10428235338105589No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA17840.1042239318945679No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC17790.10393182446212798No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG17730.10358129554320006No Hit
CCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTCTGAGCATTGCCAATG17540.10247128729992833No Hit
AGCTTTACTACCAGAGTTACGAAGAGCATCAAATCTTCTTTATGGTCAGC17490.10217917986748838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)