FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204323
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGAATGCTGCCATGGAGATCTGCTTGAATGTGCTGATGACAGGGCGG13815811.471839365352983No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG145901.211469016202464No Hit
CCAAGAGCATTTCGCTCACTTGCTGATCCTCAGGGACACCAAGACCTACA112420.933470505836059No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG110470.9172788363254709No Hit
CGCCCGTGATACACCAAGAAATGATTGGTGGATTACGGAACAACAACGAG97970.8134860830524702No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC96920.8047674917775381No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC89760.7453150027027633No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG86700.7199065367015327No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT80520.6685913994833612No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC69240.5749288189298054No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG68590.5695315957596094No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC57560.47794487027151356No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC57470.477197562447948No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC55270.4589300378718998No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC54320.4510417886231518No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG53420.4435687103874957No Hit
GTGAAGACTTTGAACGCACTCCTCAGAGTCCGACTCTCCCACCTGCTAAG52020.4319439220209197No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG51750.42970199855022284No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC50690.4209003730726723No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC48260.40072306183640105No Hit
TCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAATGCAA47410.393665154613837No Hit
GCCGAGAACATGTTGCTCACCTGCTGTTCCTTAGGGACACCAAGACCTTG46550.38652421318865454No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC44000.3653504915209624No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC40590.3370358284280878No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC39090.32458069803532774No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG38370.3186022354468029No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA37510.3114612940216204No Hit
CTGGTCTCTGGACACTATGGGCACACGACTCCTCCCAGCTCTGTTTCTTG37170.3086381311325948No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA35310.29319376944557235No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG35230.2925294958246251No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG34550.2868831700465739No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG34460.28613586222300824No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG34420.2858037254125347No Hit
CAAAGTCCGCCAAGGCACCCTGAAGAAGGCGCGCTACAATGCTCAGTGCC33100.2748432106669058No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG32220.26753620083648655No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC31780.26388269592127694No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC31560.2620559434636721No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC30920.2567417544960945No Hit
AGACTCACAGAACACAGTTGAAGAAGGAAAGTGGCGATGGACCTCATCCC30280.25142756552851686No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG29990.24901957365258323No Hit
CGCGAACGAGGCAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTG29170.2422107690378744No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG29120.24179559802478237No Hit
CTGAAGAATGCTAGCCCATCTGGCTGCCGATCTGCTATCACCTGCAACTC28540.23697961427291514No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG27560.22884226241631192No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC26460.21970850012828785No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG26230.21779871346806462No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG26110.21680230303664383No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC25870.2148094821738022No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC25720.21356396913452622No Hit
CCAGGAATATGAACAAGCCGCTGCTTTGGATCTCTGTCCTCACCAGCCTC25680.21323183232405263No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC25310.2101595668271718No Hit
CTAGCTTCAGGTGTCACCATCACGTCCTGGAAGAATAAGGAGTGGAGCTC25180.20908012219313254No Hit
CTGCAGAACCATGGAGCTTTGTGAAGAATGCCACCTCGTACCCTCCTATG24970.20733640393814615No Hit
GTTTGGAGATGTCCCAGAATAAGGCACAATGTCAATAGCAGGAGTTGCTG24140.2004445651208189No Hit
GCCCAGCGGCATCAGGCTTTCTTAAAGTGAGAGCACGCCTGTACTAGAGC23940.1987838810684509No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG23660.1964589233951357No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG22280.18500020343379642No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC21510.17860656983217957No Hit
GCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCC20980.17420575709340436No Hit
GGAGAAGTTGAGACAGGCGGTCAAGCAGGTGCTGAAGTGTGATGTGACTC20880.17337541506722035No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20650.17146562840699714No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC20480.1700540469624843No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG20290.1684763971127347No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG20250.1681442603022611No Hit
GCAAGTGGATCACAGATTCTAGTGCCAGAAAGTCAAATCTCACCAGTCGG19950.1656532342237091No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG19940.16557020002109069No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG19290.16017297685089465No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT19190.15934263482471064No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG19100.15859532700114504No Hit
ATGGCTTCCAGAGGCCGACGATCTACTATGTGATGTCAGGGCCTGCGTGG19070.15834622439328983No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG18880.15676857454354023No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG18870.15668554034092183No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG18740.15560609570688264No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC18620.15460968527546182No Hit
GCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAG18550.15402844585713302No Hit
CATGCTTGGGCTGACCCAGCATCTCCCAGCAAGACCTCATCTGTGGAGCT18320.1521186591969098No Hit
GCACTGCTTTCATTTAATTGGGCTTTAGGCACTCCATTCGAATGCAGAAC18230.1513713513733442No Hit
CGGTCGTGTGGTACTTATTCAGCGCCGGAAATGAGGCCGATGTACATGGA18200.151122248765489No Hit
GAACCGCCCATTCCTGTTTGCTGTGTATGATCAAAGCGCCACTGCCCTGC18150.15070707775239697No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG18110.15037494094192339No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG18030.14971066732097618No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG17710.14705357283718737No Hit
GAAGAAGGCTTGGAACCTGTCTGCTTTGAAAGGACTGATGACATCGGAGG17650.14655536762147697No Hit
GCATTGGAGCCTTGCCTTGCTGCTCTACCTCCACCATGCCAAGTGGTCCC17560.14580805979791137No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC17360.14414737574554334No Hit
GAGAATCCGGAAATGGTGGACCTGCCCGAGAAACTAAAGCACCAGCTGCG17210.14290186270626734No Hit
CTGAGTGGAGTGAGTTGGAGAATCTGGTCCTTAAGGATATGTCAGATGGC17070.14173938386960971No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT16610.1379198105491633No Hit
GATGTCCCTGATTGTCGAAGAACCATTTCGGCTCCTGTCGTTAGACCTAA16600.1378367763465449No Hit
CAATCTCGCCACCCTGAACCTCAACAGTGGCTCCAGTGGGACTTCGGATC16470.1367573317125057No Hit
AATGTCCTCGTACGCCTTCTTCGTGCAGACCTGCCGGGAAGAGCACAAGA16040.13318686099991447No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC15290.12695929580353443No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG15290.12695929580353443No Hit
GGCCAAGTGGGAGATCTTCCTCTTCCTGGCCATCCTGCTACAGCAACTGG14940.12405309871189041No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG14750.1224754488621408No Hit
GGACGCACAGCATGAGTCTGGACGGAGTAGCTCCAAGAGCTCTCACTGTG14620.1213960042281016No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG14420.11973532017573359No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG14410.1196522859731152No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC14090.11699519148932636No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG14060.11674608888147117No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG14040.11658002047623436No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC13950.11583271265266876No Hit
GAGAACTCCCTATAGCGATGGCTCTGGCCAATGTGGTTCCTTGTTCTCAG13810.11467023381601116No Hit
GGTAGCCGGACTCAGCTGCGAGGAGATGGGCTTCCTCAGGGCACTGACCC13730.11400596019506394No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG13610.11300954976364314No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG13580.11276044715578795No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT13520.11226224194007754No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG13490.11201313933222234No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC13480.11193010512960393No Hit
GGCAAGAAGGCAGCTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTAC13360.11093369469818314No Hit
GGAGTCGCTACCCACATAAGCCTGAAATCAACTCCACTACCCATCCTGGG13310.11051852368509113No Hit
CCACCAATGCCTGCTACGGTGGTACTGCCTCCCTCTTCAATGCTGCCAAC12940.1074462581882103No Hit
CTGGAAACTCTTCTACAACTACGCCCTGGTCATCTTCGAGATGACCAATC12910.10719715558035511No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG12830.1065328819594079No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG12790.1062007451489343No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG12680.10528736892013191No Hit
TGTGACCGAACTCAACGGCGACATAATCACCAATACCATGACATTGGGTG12670.10520433471751349No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG12470.10354365066514548No Hit
AGTTGAAATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATAGC12470.10354365066514548No Hit
CCTGAGGCAGAGTTGAGAATGGAGAGAATGTTACCTCTCCTGGCTCTGGG12430.10321151385467187No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG12260.10179993241015907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGGT351.4427133E-744.01333632
GATCGCG600.044.01333630
GCTATTT408.290954E-944.01333629
ACTCTTA408.290954E-944.01333631
TATGCTT254.435025E-544.01333631
ATCACGA408.290954E-944.01333630
TGCGCTC254.435025E-544.01333629
TGCGATA207.844914E-444.01333632
ATAAGTT254.435025E-544.01333630
CTCGAAT207.844914E-444.01333629
ATAGTAC254.435025E-544.01333631
ATGTTAT700.044.01333630
GCGTATA254.435025E-544.01333632
CCGATTA207.846519E-444.0115135
CTATACA207.846519E-444.0115135
TTCGTCC207.846519E-444.0115135
TCGATTT408.294592E-944.0115135
CATAGTT302.523273E-644.0115135
CTCGGTA207.846519E-444.0115133
TCATACG207.846519E-444.0115133
Produced by FastQC (version 0.11.5)