FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1058785
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTC38965636.80218363501561TruSeq Adapter, Index 6 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC17019916.074934949021756TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC185421.7512526150257133TruSeq Adapter, Index 6 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTG114321.0797281789976247TruSeq Adapter, Index 6 (97% over 49bp)
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC92250.8712817049731533No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGT70180.6628352309486818TruSeq Adapter, Index 6 (100% over 50bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG65850.6219392983466898TruSeq Adapter, Index 6 (100% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGCCGTC44510.4203875196569653TruSeq Adapter, Index 6 (98% over 50bp)
CGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG42400.4004590167031078TruSeq Adapter, Index 6 (100% over 49bp)
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG42030.39696444509508544No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG41870.3954532789943189No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATGTCGTATGCCGTC38940.36778004977403345TruSeq Adapter, Index 6 (98% over 50bp)
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC36350.3433180485178766No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG34320.32414512861440237No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC34320.32414512861440237No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC34050.321595035819359No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGCCGTC31690.2993053358330539TruSeq Adapter, Index 6 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGGC30730.29023833922845527TruSeq Adapter, Index 6 (98% over 50bp)
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA30390.28702711126432656No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA28220.26653192102268164No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC27000.2550092795043375No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC25090.23696973417643807No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC24610.23243623587413878No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG24360.23007503884169117No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCGCGTATGC23970.22639157147107297TruSeq Adapter, Index 6 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGCCGTC22060.20835202614317352TruSeq Adapter, Index 6 (98% over 50bp)
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG21070.19900168589468115No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG21020.19852944648819168No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG20540.19399594818589233No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG19450.18370112912442094No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATGTCGTATGC19260.18190661937976077TruSeq Adapter, Index 6 (98% over 50bp)
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC19250.18181217149846285No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC18830.17784536048395094No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC18390.17368965370684322No Hit
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC18350.17331186218165162TruSeq Adapter, Index 6 (98% over 50bp)
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC18330.1731229664190558No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG18310.17293407065646No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG17980.1698172905736292No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTTTGC17530.16556713591522357TruSeq Adapter, Index 6 (98% over 50bp)
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT16930.15990026303734942No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC16740.15810575329268925No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC16060.15168329736443187No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTAAGCCGTC16020.15130550583924027TruSeq Adapter, Index 6 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCCTC15910.15026657914496333TruSeq Adapter, Index 6 (98% over 50bp)
GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG15800.1492276524506864TruSeq Adapter, Index 6 (100% over 49bp)
TTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC14720.1390272812705129TruSeq Adapter, Index 6 (98% over 50bp)
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGGCGTC14290.1349660223747031TruSeq Adapter, Index 6 (98% over 50bp)
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC13720.12958249314072262No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC13640.1288269100903394No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG13400.12656016093918973No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA13050.12325448509376312No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG12970.1224989020433799No Hit
CTCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC12840.12127107958650718TruSeq Adapter, Index 6 (98% over 50bp)
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC12830.12117663170520927No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGG12650.11947656984184701TruSeq Adapter, Index 6 (97% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATATCGTATGC12380.11692647704680366TruSeq Adapter, Index 6 (98% over 50bp)
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC12100.11428193637046237No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC12060.11390414484527076No Hit
CCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG11880.11220408298190851TruSeq Adapter, Index 6 (98% over 50bp)
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC11610.10965399018686513No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG11550.10908730289907773No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG11540.1089928550177798No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG11390.10757613679831127No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC11380.10748168891701337No Hit
GAAGTTCTGCGCTGGTCGGCGGAGTAGCAAGTGGCCATGGGGAGCCTCAG11340.10710389739182176No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC11130.1051204918845658No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATTCCGTC10960.10351487790250145TruSeq Adapter, Index 6 (98% over 50bp)
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG10960.10351487790250145No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC10880.10275929485211822No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC10800.10200371180173501No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG10640.10049254570096855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAGT1050.044.004822
GGGTAAG1050.044.004821
TTGATCG351.444696E-744.0048181
AGCCGTA254.4397315E-544.00481844
GGAATCG207.851965E-444.00481844
GTTCTAA1300.044.0048182
CGACGCA750.044.0048181
GGCGTTG551.8189894E-1244.00481844
TAGCGCT600.044.00481844
GAATCGG650.044.0048181
GTTAGTA650.044.0048181
TCTAGAC408.303687E-944.00481844
AGTATCA254.4397315E-544.0048182
ATCGTAG408.303687E-944.00481844
AGTTGAA302.5256231E-644.0048181
GAGCGAA454.783942E-1044.0048182
CCATATA254.4397315E-544.0048181
GTCCGGA750.044.0048182
TTAGTAA650.044.0048182
CCGGTCT1500.044.0048181
Produced by FastQC (version 0.11.5)