FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694205
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC925855.464805026546374No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA498332.9413795851151425No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG439462.59390097420324No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG238051.4050838003665436No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG192051.1335700225179361No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG184981.0918395353572914No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT135340.798840754218055No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC132580.7825499275471387No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC118950.7020992146759099No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC117200.6917698861707999No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC115690.6828571512892478No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC101390.5984517812189197No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC97270.5741335906811749No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG91470.5398992447785245No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC86750.5120395701818847No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG83150.4907906658285155No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG80800.4769198532645105No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC78040.46062902659359406No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG76540.4517753164463569No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG75830.4475845603099979No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG72920.4304083626243577No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC68540.4045555289944251No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC68360.40349308377675663No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG68050.40166331701299424No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC64100.378348546958603No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG63320.37374461768203965No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG60850.3591655083062557No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA59130.34901325400409045No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA57950.34204833535493046No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC57060.33679513400090305No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC54930.3242228655918263No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG54220.3200321094554673No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC52340.30893545940426337No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG51300.3027968870355122No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG51100.3016163923492139No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC51000.3010261450060648No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC50900.3004358976629156No Hit
CATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTC50710.29931442771093225No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG50040.295359770511833No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC48980.289103148674452No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG47410.27983626538701045No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG47120.2781245480918779No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC47020.27753430074872876No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC46190.27263524780059084No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC45420.2680903432583424No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG45330.26755912064950815No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG44780.26431276026218786No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG44440.26230591929548075No Hit
GAAGTTCTGCGCTGGTCGGCGGAGTAGCAAGTGGCCATGGGGAGCCTCAG43490.2566985695355639No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG41910.24737266151380735No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC40420.23857797610088508No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC40420.23857797610088508No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG40390.23840090189794033No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG39280.23184915638898482No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT39200.23137695851446546No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC37710.2225822731015432No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG37670.22234617416428357No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC37480.22122470421230017No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG36680.21650272546710697No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC36620.2161485770612175No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG36170.21349246401704633No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG35990.2124300187993779No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG35980.21237099406506296No Hit
CCCACTTGATTAGAGCAATGGGAAGCATACTGTGGCCTACCAGCATCTGG35570.20995097995815146No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC34620.2043436301982346No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC34280.20233678923152748No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC33760.1992675030471519No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG33410.1972016373461299No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG33330.1967294394716106No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG33150.1956669942539421No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC32260.19041379289991472No Hit
CTGCCTCAATCCTAGGTAACGAGGGCAACAGGGAGCACCTGTCTGGATTG31890.18822987773026287No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC31180.18403912159390393No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT30010.17713322767905892No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT29800.1758937082584457No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG29800.1758937082584457No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG29770.17571663405550098No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG29580.17459516410351758No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG28670.16922391328086034No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC28380.1675121959857278No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC27970.16509218187881633No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG27780.16397071192683293No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG27520.16243606883464515No Hit
CAACAGGGTCAGCTTCTGGAGGTTGGTTGGAACAATACGGCAAGTGCTCG27290.1610784999454021No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG27220.1606653268051977No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC27160.16031117839930822No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC26900.15877653530712046No Hit
CATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGCGCT26840.15842238690123098No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC26650.15730091694924758No Hit
GTCTGTAAGGCGTAGAATCTGTCCGTCTGTAAGGCGTAGAATGAGGGTTG26620.15712384274630284No Hit
CGCGAACGAGGCAAACTATGCAAGAGGCACCAGACTTCCTCTTTCTGGTG26550.15671066960609845No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC26440.15606139752863438No Hit
CTGGCTTGACCACTGGATGCTTTGCCGAAAGCAGCTTGGCTTGGTAGCTC26340.1554711501854852No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC26230.15482187810802117No Hit
GTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCG26130.15423163076487204No Hit
CGGCGAGAGCGTATGGAGCCGAGCCGTTAGCGCGCGCCGTCGGTGAGTCA25670.15151649298638595No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC25440.1501589240971429No Hit
GAATGGCTGATCATCTTGGCATCCCTCTTGGCCTTGGCTTTGATTCTTGC25100.14815208313043582No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG24860.14673548950687787No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG24560.1449647474774304No Hit
GCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACGAGCC24450.14431547539996636No Hit
GGCCACTTTCCCTGTGTACACCATGGGCGCTATTCCAATTGTTTGCAAGG24290.14337107965092774No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG24210.14289888177640842No Hit
CCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTCTGAGCATTGCCAATG23360.13788177935964066No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG23260.13729153201649152No Hit
TGTCGTGTCATACTCGAGTCTGAGGCCGTTGCTGACTTAGAATCCCTGTG23120.1364651857360827No Hit
CAAGCCTTCTCCTGAGGAAGCACAGCTGTGGTCAGAAGCATTTGACGAGC23120.1364651857360827No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC23090.13628811153313797No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC22840.13481249317526509No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC22730.13416322109780104No Hit
GCGAGGATTAAGCAGGATAATGCATGTAAAGTGTTAGCACAGTGTCTCAC22610.13345492428602207No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA22370.13203833066246412No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG22330.13180223172520444No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG22120.13056271230459124No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG21840.12891001974377364No Hit
TAACCCTCAGCATGCTAGGAGAGTGCACCCGTACCTATGGAAGTGGTAAA21590.12743440138590076No Hit
GGCTGGAGTACGATGACCTGAGGAGAGGACGAAGCGAAGCGGACAGTGAT21240.12536853568487877No Hit
AGTTGAAATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATAGC21030.12412901626426553No Hit
GACAAATGCGACGAACCTCTGAGCATCCTGGTGAGGAATAACAAGGGCCG21010.12401096679563572No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC21000.1239519420613208No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG20990.12389291732700589No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG20920.12347974418680148No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC20890.12330266998385675No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG20640.12182705162598387No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG20580.12147290322009438No Hit
GGAGATTGGTCACATGTTCAGTTGTCTGAGATACTGGTAAATGTGCGCTG20330.11999728486222151No Hit
CCGCAGGATATACGCCAACATGTTCAAGAAGTTTGCAGAGCAGGATGCCA20210.11928898805044254No Hit
CACGCAGCTTCGGTTGCAAAGACTTTCTTGATGTCGGACTGCGTTGTTGT19900.11745922128668018No Hit
GCTGAAGGCATGTACCTACAAAGGTCACAAGACAGTTGTCACTGGAGAGC19840.1171050728807907No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT19550.11539335558565816No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG19420.11462603403956427No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC19410.11456700930524935No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC19410.11456700930524935No Hit
CCGCCACCGCATCGCGCAGCTCTTTGCCGTCGGAGCGCTTGTTTGCTGCC19380.11438993510230462No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG19220.113445539353266No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG19160.11309139094737651No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG18920.11167479732381855No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC18780.11084845104340973No Hit
GCATTTCGATGGTGGCAACCGCAACATGGGAAGCATTCAGCCCAGTGGGC18400.10860551113944297No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC18370.10842843693649824No Hit
CTTGGGCCGACCACTCTGTTCTCATTCTCCTTCCCACCAACATCCATCCG18310.10807428853060876No Hit
GCAGTGCTAAAGCCTGTGGGTTTGGTCCTTGAACAAGATGTGGGCCTTGC18250.10772014012471927No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG17930.105831348626642No Hit
GCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTG17660.1042376808001393No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG17540.10352938398836033No Hit
CTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGG17460.103057186113841No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT17430.10288011191089627No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG17170.10134546881870848No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG17110.100991320412819No Hit
CCAGGCCAACCTTGGCTAAATGGAGCAGCGAAATCAACGAGAAACTGGAC17090.10087327094418916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTAA2000.044.00670232
ACTCTTA254.4397428E-544.00670231
TAAACGG1000.044.00670230
TTATATT454.802132E-1044.00670231
TTCATAA302.5257814E-644.00670229
ATAGTAC351.444896E-744.00670231
GCGTATA502.7284841E-1144.00670232
TAGTACG750.044.00670230
CACGCGA408.305506E-944.006732
TGCGATT207.85165E-444.006729
TACTAAA408.305506E-944.006730
ATTTAAG302.5262943E-644.00540537
AGCGTTG351.4452417E-744.00540542
CGTATTA650.044.00540538
GTATTAG650.044.00540539
ATAGCGT351.4452417E-744.00540540
CCGTATT650.044.00540537
CTCGGTA302.5262943E-644.00540533
ATATAGC351.4452417E-744.00540538
TCTAACC650.044.00540539
Produced by FastQC (version 0.11.5)