FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1679272
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG352112.096801471113673No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG187031.113756437313312No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC143270.853167324888404No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC137580.8192835943194431No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG100030.5956747924100444No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG96230.5730459389544994No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC95100.5663168325321926No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC94920.565244939473772No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC88330.5260017436127084No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC87220.519391736419115No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC87050.5183793929750511No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA80390.4787193498134906No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC80070.4768137621540763No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC79830.47538457140951557No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC77080.4590084274614238No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG76870.45775788555993313No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG71860.42792352876722767No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT68160.40589017145524964No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC67110.3996374619477964No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG65730.39141961516657214No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC64610.3847500583586221No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC63740.3795692419095894No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG62120.3699222043838044No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG55940.33312054271136543No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC55410.32996441315046043No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG52070.3100748419553235No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG50970.3035243843760868No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG49270.29340094993544824No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA48170.28685049235621146No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG47460.282622469736886No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC46470.27672705791557295No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC45080.268449661519992No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC44970.2677946157620683No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC42530.25326450985903415No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG41370.24635675459365727No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG40180.23927035048521028No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG39380.23450638133667448No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC38140.227122229156444No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC38090.22682448108466052No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG36550.2176538404737291No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG36410.21682014587273532No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC36140.2152123062851045No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC34030.20264733765584134No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG33470.19931255925186628No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC32300.19234525437213268No Hit
GAAATACAGCCCTTGTGCCTGGGAGGTTGTCAGAGCAGAAATCATGAGAT31940.19020146825529158No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC31340.18662849139388973No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG31250.18609254486467947No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG31040.1848420029631888No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG30800.18341281221862807No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG30600.18222181993149414No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG29060.17305117932056271No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG28740.1711455916611484No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG28700.1709073932037216No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC28650.17060964513193813No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC28290.168465859015097No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG28010.1667984698131095No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC27660.16471423331062507No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC27370.16298729449428084No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACGCCTAAATCTCGTATGCCGTC26980.16066485953436965TruSeq Adapter, Index 6 (97% over 34bp)
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG26590.15834242457445846No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC26540.15804467650267498No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG26520.15792557727396156No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC25600.15244701275314543No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG25450.15155376853779495No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC24940.14851673820560338No Hit
CGAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTG24800.1476830436046096No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG23110.13761915877832775No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG23000.13696411302040407No Hit
GTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGC22890.1363090672624804No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG22840.13601131919069692No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG22710.13523717420405984No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG22320.13291473924414865No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG22130.1317832965713714No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC22120.1317237469570147No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT22040.13124735004216112No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC21780.129699060068887No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG21600.12862716701046642No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG21520.12815077009561285No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG21480.12791257163818606No Hit
CTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGG20510.12213625904558642No Hit
CTCTGCACACTCCAGCCCACTTTCTGCCTTCAGCCATTGAGTGGAAGCTG20310.12094526675845248No Hit
GAGTTCCAGACCAGTGTTGTCTCCCGGGACATTGACACAGCAGCCAAGTT20110.11975427447131852No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG19760.11767003796883413No Hit
GCCGTCCATCACAACTGAGATCCTCAAGTCAGTGGTGGCTCGCTTTGATG19440.1157644503094198No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC19240.11457345802228586No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC18900.11254877113415815No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG18580.11064318347474382No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT18220.1084993973579027No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG18140.10802300044304913No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG18130.10796345082869244No Hit
CCATTCGTTCGTGAGTGTCCCGTGCACGTCCTGACCCTGGACAGTGAAGG18070.10760615314255224No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC18010.10724885545641206No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG17970.10701065699898528No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC17940.10683200815591518No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG17760.10576011509749464No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC17750.10570056548313794No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG17720.10552191664006784No Hit
GAGAAGTTCCCTTCACCGCATCCGTCGCCTGCCAAACTGAAGGCTACTGC17700.10540281741135443No Hit
AGAAACAGCGATGGACTCCACTGGAGATCAGCCTCTCGGTCCTTGTCCTG17490.10415227550986379No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG17460.10397362666679369No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG17390.10355677936629683No Hit
GGACCCAAAGGACGAAATGTTGTTCTTGAGAGCAAGTATGGATCACCACG17290.10296128322272985No Hit
GCCGGCCTCGGCAATCATCTCTCTGGAAGTGAGCGGTTGACCCTCCTGGC17210.10248488630787626No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG17130.10200848939302268No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT16950.10093659633460214No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG16900.10063884826281866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG750.044.01275630
TTTATGA750.044.01275632
TTCAATT351.4433317E-744.01275330
GTTAGAT302.5233803E-644.01275331
CAAACGT207.846316E-444.0127530
CGCGCCT207.846316E-444.0127531
TATATAG502.7284841E-1144.0127532
TATGCTT502.7284841E-1144.0127531
TTATCGT207.846316E-444.0127532
TTATATT408.294592E-944.0127531
AAACGTA207.846316E-444.0127531
TATCGAT254.4361244E-544.0127531
CTAGATA302.523897E-644.0114435
CGGAATA454.783942E-1044.0114434
TTAATGC502.7284841E-1144.0114434
CTACTAA302.523897E-644.0114433
CCGATTA551.8189894E-1244.0114435
TAGACTA850.044.0114434
ATTACTT207.847467E-444.0114434
CTCCCCA207.847467E-444.0114435
Produced by FastQC (version 0.11.5)