FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386425
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC245731.7724002380222517No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG219871.585877346412536No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC143391.0342427466325261No Hit
AACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGG140591.0140469192347223No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT112850.8139639720864814No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC103970.7499143480534468No Hit
CTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAGCTGC103260.7447932632490037No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG102080.7362821645599293No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG100990.7284202174657843No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG84460.6091927078637503No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG81040.5845249472564329No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC74420.5367762410516256No Hit
CTGTGAATCACTGCAGGCTGTGTTCTTGCATCTTGTCTGCAAACAGGTCC71830.5180951007086572No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC68610.4948698992011829No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG67700.4883062552968967No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG67110.48405070595235955No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG62310.44942928755612455No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA60680.43767243089240315No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC60460.4360856158825757No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC59670.4303875074381953No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG56250.405719746830878No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC52570.37917665939376455No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC51210.3693672575148313No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC49790.35912508790594516No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC47440.3421750184827885No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG46840.3378473411832591No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG46620.33626052617343166No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC45280.32659538020448275No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC44860.3235660060948122No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG44260.3192383287952828No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG42410.3058946571217339No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG41600.30005229276736933No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG41540.2996195250374164No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG40830.2944984402329733No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC40100.2892330995185459No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG39730.2865643651838361No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC37950.2737255891952323No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG37950.2737255891952323No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG36790.2653587464161422No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT35500.2560542402221541No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG35100.2531691220224679No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC34730.2505003876877581No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC34380.24797590926303265No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG32490.23434372576951512No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC31540.22749157004526027No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA31240.22532773139549558No Hit
CGACTACATCAGGGACCTTCAGTTGGAGCTGAACTCGGAATCCGAAGTTG31020.22374091638566818No Hit
GGCAATGAGTCCAACGGGCATGAGTCTAGAGGCGCATCTCAGCGGAGCTC30830.22237048524081723No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC30300.21854770362623294No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG29060.20960383720720557No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC28950.20881042970229188No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT28480.20542041581766055No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG28100.20267955352795858No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC26930.19424058279387635No Hit
CACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTC26530.1913554645941901No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC26120.1883982184395117No Hit
GGAAGGATTTCGCTGCTTCCCGAAGGTCTTGGACGAGCGCTCTAGCTCTG25600.1846475647799196No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG25470.18370990136502155No Hit
CCTGCCCACTTGACTTCACCAAATCCCTTCCTGGAGACTAAACCTGGTGC25410.18327713363506862No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG25250.1821230863551941No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA25240.18205095840020197No Hit
GAAGGAACTCCTGTTGCACATCCGAAGTCATAAACAGAAGGCTTCTGGCC25140.1813296788502804No Hit
GACCAGTCGATGGCAGAATTCCACGGCAGTGGTCTAAAGCATTACCTTCT25100.18104116703031178No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG25090.1809690390753196No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT24640.1777232811006726No Hit
GCTGCTAGACGTGAAGCACTTCTCGCCGGAGGAAATTGCTGTCAAGGTGG24380.17584795427087654No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC24170.17433326721604125No Hit
GTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGC24050.17346773175613536No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG23650.17058261355644913No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG23550.16986133400652756No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC23490.16942856627657463No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT23230.16755323944677858No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA22700.1637304578321943No Hit
GACGCAACCCACGTGTAACTGTCAGCCGGGCCCTGAGTAATCGCTTAAAG22530.16250428259732766No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG22310.16091746758750022No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC22300.16084533963250808No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC22300.16084533963250808No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT22190.16005193212759436No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG21860.1576717096128532No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG21760.15695043006293163No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC21490.15500297527814344No Hit
AGAGCCAACATGTTGGTATTGCTGGCTGGTATCTTTGTGGTCCACATCGC21480.15493084732315127No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG21200.1529112645833709No Hit
CCTTTCTGATCCGCGACAGCTCGGACCAGCGCCACTTCTTCACGCTCAGC21050.15182934525848857No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC19750.14245271110950827No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG19680.14194781542456317No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC19280.13906269722487694No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC19150.1381250338099789No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG18970.1368267306201201No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG18830.13581693925022992No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC18820.13574481129523774No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG18790.13552842743026128No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC18420.1328596930955515No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC18340.13228266945561426No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC18340.13228266945561426No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC18250.13163351786068486No Hit
TGGAAGAGGTCCAGGATGGCAAGGTCATCTCCTCCCGCGAGCAGGTCCAC17760.1280992480660692No Hit
GGTTCCCGTTTCTCCACCTAGACTGTAAACCTCTCGAGGGCAGGGACCAC17720.12781073624610056No Hit
CAACATGAGACCAAAGTGTGTGACTAGTCAATGAAGTGCGACAGCATTAA17490.12615179328128098No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG17370.12528625782137512No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG17210.12413221054150061No Hit
CCGCAGCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTG17160.12377157076653984No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT17110.12341093099157907No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC16670.1202373009719242No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC16460.11872261391708892No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG16450.11865048596209676No Hit
CCCACTCAGTGAAATGACCCGCGAAGAGATCAATGCGCTAGTGCAGGAGC16380.11814559027715167No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC16260.11728005481724581No Hit
CAAGGTCTCCTGATCCGTCCGAGGCTGCTTCCCAGAGGAGCAGCTCTCCC16240.11713579890726149No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG16100.1161260075373713No Hit
CCTTGTGGATATGCTGTGTGAACCGTCGTGTGAGTGTGGTATGCCTGATC16020.11554898389743405No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC15750.11360152911264584No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT15640.11280812160773211No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG15630.11273599365273995No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG15440.111365562507889No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG15420.11122130659790468No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG15250.10999513136303804No Hit
CCAGGACGGAGACCTCACCCTGTACCAGTCCAATACCATCCTGCGTCACC15030.1084083163532106No Hit
GCACCATCCCAGTGATGTTCTGGCAGGATTTGCTCAAGGAGCCCTGGTGG14970.10797554862325764No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG14820.10689362929837531No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC14810.10682150134338317No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG14770.10653298952341453No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC14750.10638873361343021No Hit
GGCATTCGGAAAGTATTGTCGGCCAGAGAGCCAGGAGCATCCTGAAGCTG14710.1061002217934616No Hit
GCCTCAACCTGGAGGCCATCAACTACATGGCAGCTGACGGTGACTTCAAG14690.10595596588347728No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG14680.10588383792848513No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG14670.10581170997349298No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG14630.10552319815352434No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC14590.10523468633355573No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC14560.10501830246857925No Hit
CAGCCAGCCAAGGCGGACAAGGAGAGCTTCGAGAAGGCGTACCAGGTGGG14350.10350361541374398No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG13980.10083488107903421No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC13970.10076275312404205No Hit
CTGGAACAGCCAGTCGTCCTTGCTGGATGTGCAACGGGTTCCTTCCTTCG13910.1003299853940891No Hit
CCGCTGACCACCCTCAACACGGATATCAGCATCCTGTCCTTGCAGGCTTC13870.10004147357412049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT408.294592E-944.012051
GTTAGTA1200.044.012051
CCATATA950.044.012051
AACGATA650.044.012051
CACGGCG351.4436773E-744.0104643
GCGCGTC302.5239624E-644.0104644
TTGCTAA254.4370834E-544.0104646
ATCGTTG408.296411E-944.0104646
TATTGTA4250.044.0104644
TTATAGT207.84785E-444.0104644
ACGATAG700.044.0104642
TCGTGAC502.7284841E-1144.0104642
ACAATCT302.5239624E-644.0104645
GAAACGT1250.044.0104642
GGTATTA207.84785E-444.0104646
TGTTATA551.8189894E-1244.0104646
AATCTAC254.4370834E-544.0104642
GGCGGGC207.84785E-444.0104642
CGTACGT254.4370834E-544.0104644
ATGCGCG254.4370834E-544.0104645
Produced by FastQC (version 0.11.5)