FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132920
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC209341.8477915475055606No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC157471.3899480987183561No Hit
CCCTAGAGGGTTCCTGTAGACCTAGGGAGGACCTTATCTGTGCGTGAAAC106980.9442855629700243No Hit
CAGGAGCGATGAACGCAAGAGGCATACCAAGATCCACTTGCGGCAGAAGG102770.9071249514528827No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG89580.7907001376972778No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC82890.7316491897044805No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC82700.7299721074744907No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG79420.7010203721357201No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG79300.6999611623062529No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG78710.6947533806447057No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT68830.6075451046852381No Hit
GTCTCCATCCCTGTCCACTCCACGAACAGTGCGCTGAGCTACCTTCAGAG64200.5666772587649613No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA61250.5406383504572255No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC59470.5249267379867951No Hit
CTTCATGGACGGCTACACAGGAGAGTTTGACACCTTCCTCTACCAGCTGC51270.4525473996398687No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG49760.43921900928573954No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG49720.4388659393425838No Hit
GAATAAGCTAGCCCAGCCACACCACCTTGTTGTGTGACCTTGGGCAGGTG49610.43789499699890555No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG49170.43401122762419236No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC47850.4223599195000529No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC46950.4144158457790488No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG46220.4079723193164566No Hit
GCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGC46180.40761924937330085No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG40690.35916039967517566No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC40530.3577481199025527No Hit
CATGAACCACGTTAACAGTGGGTGCCAACTCATGCTAACGCAGCAGTTGC39950.3526286057267945No Hit
AATGAAATTACAGGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAC39490.3485683013805035No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC37310.32932598947851566No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG37090.32738410479115915No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG36860.3253539526180136No Hit
AACAGCCCTTCTACCACGACGACTCATACACAGCTACGGGATACGGCCGG36860.3253539526180136No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG35320.3117607598065177No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC34690.30619990820181475No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG34100.30099212654026763No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC32030.28272075698195814No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG29150.2572997210747449No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC28790.25412209158634325No Hit
CACAATGAGCTCCCGCATCGCCAGGGCGCTCGCCTTAGTCGTCACCCTTC28750.2537690216431875No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC28590.25235674187056456No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG28430.2509444620979416No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG27940.2466193552942838No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC27640.24397133072061578No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG27570.24335345832009322No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA27000.23832221163012393No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT26530.23417363979804398No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG26220.231437347738587No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG26170.23099601030964234No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC26100.23037813790911982No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC25900.2286127881933411No Hit
CTGTGAATCACTGCAGGCTGTGTTCTTGCATCTTGTCTGCAAACAGGTCC25880.22843625322176322No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC25580.2257882286480952No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC25260.2229636691028493No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC25220.22261059915969356No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG23840.21042968612082052No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC23800.21007661617766482No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG22680.2001906577693041No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA22420.1978957031387918No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG21810.1925113865056668No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA21730.1918052466193553No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC21620.19083430427567702No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC21580.1904812343325213No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG20540.18130141581047204No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG20380.1798891360378491No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG20370.17980086855206018No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC20300.17918299615153763No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG20100.1774176464357589No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG19910.17574056420576917No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG19080.16841436288528758No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC18980.16753168802739823No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC18890.1667372806552978No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG18810.16603114076898634No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG18290.16144123150796172No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC18280.16135296402217278No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC17920.15817533453377114No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG17780.15693958973272606No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT17690.15614518236062566No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG17620.1555273099601031No Hit
GGAAGGATTTCGCTGCTTCCCGAAGGTCTTGGACGAGCGCTCTAGCTCTG17510.1545563676164248No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG17440.15393849521590225No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC16890.14908378349751086No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG16880.14899551601172192No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG16620.14670056138120963No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC16530.1459061540091092No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC16420.1449352116654309No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG16180.1428167920064965No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG16030.14149277971966248No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG15860.1399922324612506No Hit
AGATCAGTGTACCATTTGCCTCCCGGGCTCAAGCGATTCTCCTGCCTCAG15680.13840341771704973No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG15580.1375207428591604No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG15580.1375207428591604No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG14980.1322246937118243No Hit
CGAGGGTCCGCTTCGTCTTTCGAGAGTGACTCCCGCGGTCCCAAGGCTTT14910.13160682131130177No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT14900.13151855382551284No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG14870.13125375136814604No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC14720.12992973908131203No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT14500.12798785439395544No Hit
AGGGATCCTCCTATGTGTGCCTCCTAGAGTGCAGGGATTACAGGCGTGAG14470.12772305193658864No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC14420.12728171450764397No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC14390.12701691205027715No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC14320.12639903964975463No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG14220.12551636479186526No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT14210.12542809730607632No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC13980.12339794513293084No Hit
GCTGCTAGACGTGAAGCACTTCTCGCCGGAGGAAATTGCTGTCAAGGTGG13920.12286834021819723No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT13920.12286834021819723No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG13860.1223387353034636No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG13820.12198566536030789No Hit
GTCTCGGATGGATAAGGTGGAACGCCTGAAACAGGTGTTGCTCCACCAAC13820.12198566536030789No Hit
CGAGTGCGAGTACTACCCGGTGGTGCCTGGGAAGTATGTGGTGACCATCA13820.12198566536030789No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC13760.12145606044557428No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT13730.12119125798820746No Hit
CAAAGTAGACAAATGGCGCCGGGCACGGTGGCTCACGCCTGTAATCCCAG13720.12110299050241853No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA13570.1197789782155845No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG13550.11960244324400664No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG13510.11924937330085089No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC13460.11880803587190622No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG13430.11854323341453943No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC13320.11757229107086113No Hit
CTGATCCTGTAACGCTGCTGGAGGTCAGTCTGAGCTACCAAGACTGTCCC13120.11580694135508245No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC13110.1157186738692935No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG12970.11448292906824842No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG12950.11430639409667055No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG12930.11412985912509269No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG12850.1134237192387812No Hit
GGCAGCCACACTTCTCAGGACACAGGCCTGTGTAGCTGTGACCTGCTGAG12690.11201143946615824No Hit
GATGGAGACAGTATGCCGGCTCACCTTGGTGACCAGCCAAGATGTCTCAA12640.11157010203721358No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG12610.11130529957984678No Hit
AAAGAGAAAGCGAGACAGGCCCGTGGACAGTGATTCCCAGACGGGCAGCG12610.11130529957984678No Hit
GGTTCGAGACTGGAGTACAAGGAAGAGCAGGGACTTCAATGAAGAGTGTC12580.11104049712247996No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT12530.11059915969353529No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC12380.10927514740670127No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG12010.10600925043251068No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG11910.10512657557462132No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG11690.10318469088726476No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC11640.1027433534583201No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC11560.10203721357200861No Hit
CCGCAGCCCTCTCTAGCTTCATGAACCCAGACAAGATCTCGGCTCCCTTG11510.10159587614306394No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG11450.10106627122833034No Hit
GACGAGGACAAGACGTCGGTCCACTTCGAGGAGGACTTCCACAAGCTCAG11430.10088973625675246No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC11400.10062493379938565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTT408.260031E-944.03116
TGTTATA302.515424E-644.03116
ATCGTTG254.4243065E-544.0310976
TATACCT254.4243065E-544.0310976
AAATATT600.044.027215
GTCCTAT351.4390571E-744.027215
AAGCGTC302.5169538E-644.027215
TTGCGCG207.832549E-444.027215
TATAGGA351.4390571E-744.027215
ATGCGCG351.4390571E-744.027215
AATCGTT302.5169538E-644.027215
GTACGGA207.832549E-444.027215
AGTCGAT1050.044.027215
ATATACT254.4266148E-544.0272065
TAGTCGG502.7284841E-1144.0272065
CGTATAT408.270945E-944.0252654
GCGAAAC302.5177196E-644.0252654
TATGCGC408.270945E-944.0252654
ATTGACG9350.044.0252654
CTATGCG351.4400575E-744.023323
Produced by FastQC (version 0.11.5)