FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651909
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC532098.162028749411345No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG162642.4948267319518522No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG85381.3096919968891363No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC76081.1670340492307976No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG73461.1268443908582333No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC60760.9320319247011469No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC60400.9265096815659855No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC48980.7513318576672511No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC48810.7487241317423138No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC45740.7016316694507976No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG43080.6608284285076599No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA40310.618337835495445No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA37810.5799889248346011No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG37090.5689444385642781No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT36270.5563659958675214No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA33450.5131084246420896No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG32240.49454755188224125No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG31320.4804351527590507No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC30020.460493719215412No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC29760.45650543250668424No Hit
CCTGGACACACGCAGATATGCTGTCTAGTCACACACACACGCAGACATGC29350.45021621115830585No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC27760.42582630397800925No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC27710.42505932576479233No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG26420.40527128786379696No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC26250.40266356193885955No Hit
GTGAAGACTTTGAACGCACTCCTCAGAGTCCGACTCTCCCACCTGCTAAG26110.4005160229418523No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG24940.3825687327525774No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG24870.3814949632540738No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC24100.3696834987705339No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC23980.3678427510588134No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG23900.3666155859176664No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC23340.35802542992963743No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC23280.3571050560737772No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC22640.3472877349446012No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG22330.3425324700226566No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG22130.339464557169789No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC22020.3377772051007119No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC21200.3251987624039551No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC21080.32335801469223463No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG20770.31860274977029No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC20500.3144610674189189No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG20500.3144610674189189No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG20230.31031938506754775No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC20080.30801845042789716No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG19380.2972807554428609No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC19340.2966671728722874No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG19270.29559340337378376No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG19050.2922186992356295No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG18940.2905313471665524No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG18230.27964025653887276No Hit
CATGGATTGGTGTTGCTGGACCTTCAGAGTTATGATGGTGATGCGCAAGG18040.27672573932864863No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG17930.2750383872595715No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG17160.2632269227760316No Hit
GTGGAGCCCAGCATTTATGGTGCCTGATTCCTGCATGGTCAGTGAGCTGC16500.2531028103615689No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG15790.24221171973388922No Hit
CCCACTTGATTAGAGCAATGGGAAGCATACTGTGGCCTACCAGCATCTGG15410.23638268531344098No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC15370.23576910274286747No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC15170.23270118989No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG14860.22794592496805538No Hit
CTGCAAACGCTCTTTGTTCCAGAGCATGGAGATCACGGAGCTGGAGTTTG14590.22380424261668422No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC14500.22242368183289385No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG14460.22181009926232034No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC14450.221656703619677No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG14410.22104312104910348No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC14180.21751502126830585No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG14060.21567427355658536No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC13820.21199277813314432No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT13680.20984523913613712No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC13590.20846467835234672No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT13560.20800449142441663No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC13110.20110168750546473No Hit
GCCGTGCAAGGCATGCTGGACTTTGACTATGTCTGCTCCCGAGACGAGCC12920.1981871702952406No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG12570.19281832280272246No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG12550.19251153151743572No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC12540.19235813587479234No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC12400.1902105968777851No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC12250.18790966223813446No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG12050.18484174938526696No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG11880.18223402346032957No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG11420.17517782389873435No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG11320.1736438674723006No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG11210.17195651540322346No Hit
GCAGTTTATGGAGGAGACAGAGCGGTCCCTGCATGATGCCATCATGATCG11180.17149632847529334No Hit
CAACGTGGTCACCACGCTCGTGCTCATCTCCTACTGTCCCACGGCCACCG10960.1681216243371391No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC10860.16658766791070534No Hit
GGCCACTTTCCCTGTGTACACCATGGGCGCTATTCCAATTGTTTGCAAGG10820.1659740853401318No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC10820.1659740853401318No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG10810.16582068969748845No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG10740.16474692019898482No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG10570.16213919427404744No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC10560.16198579863140408No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG10350.1587644901358932No Hit
CTGCCTCAATCCTAGGTAACGAGGGCAACAGGGAGCACCTGTCTGGATTG10140.1555431816403823No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG10080.15462280778452206No Hit
CTTCTTGTAGTACAGATATGTGCATTCATGTCTGTAAATTAGGACAAGAC10020.1537024339286618No Hit
AGCTTTACTACCAGAGTTACGAAGAGCATCAAATCTTCTTTATGGTCAGC9960.15278206007280157No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT9870.1514014992890112No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG9760.14971414721993406No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC9730.14925396029200394No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG9670.1483335864361437No Hit
GAAGCCTAGGAAAGACCCAGGAGTTCCAAACAGTGCTCCCTTTAAGGAGG9510.1458792561538497No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG9400.14419190408477256No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC9360.14357832151419908No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC9360.14357832151419908No Hit
GGACGCACAGCATGAGTCTGGACGGAGTAGCTCCAAGAGCTCTCACTGTG9340.1432715302289123No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG9280.14235115637305207No Hit
CGCTGGTGGATTGGAATCTTGAAGCAGGTGTACAGTGTAAAGCTGCCCTG9200.14112399123190508No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC9150.1403570130186882No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC9130.14005022173340143No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC8990.1379026827363942No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC8900.13652212195260383No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG8760.13437458295559657No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG8680.13314741781444958No Hit
CTCAGCTCTTGCCAGGCCAAATTGAGACATGTCTGACACAAGCGAGAGTG8460.1297727136762953No Hit
GCGGAACCATCTTGGCAACAGTCCCACTGGTCGTAGATGCGGAGAAGCTG8430.1293125267483652No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC8330.12777857032193143No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG8290.12716498775135793No Hit
GGGAACTCATGCACTATCTCAGGGAGGTGATGCAGGATTACCGAGATGAG8240.12639800953814104No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC8210.12593782261021091No Hit
TACTCTCACAGGTAGAATGAACTGTGTACTGGCCACATATGGAAGCATTG8210.12593782261021091No Hit
GCTGACATACAAATGCCATTCACCTGCTCGGTGACATATTATGGACCATC8200.12578442696756756No Hit
GCTTCTCCAGCTTTGTGCCTGTCACTATTCCTCATGCCACCACTGCCAAC8180.1254776356822808No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG8160.12517084439699405No Hit
AGTTGAAATGGATTGTTTCTGGGCGTACTGCACGGCAATTGAAGCATAGC8030.12317670104263019No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG7920.12148934897355307No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG7820.1199553925471193No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG7760.11903501869125906No Hit
GCACCTCTTGTCCTTTGGCTTTGTCACGAGAGTTGTGAGGAGAATGGTGG7640.11719427097953855No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC7540.11566031455310481No Hit
GGAGCAAGAGGTCAATTAGGAACTGGGCACACTTGTAATGTTAAGTGCCC7510.11520012762517469No Hit
GGTAGCCGGACTCAGCTGCGAGGAGATGGGCTTCCTCAGGGCACTGACCC7450.11427975376931442No Hit
GTGCCTCTCGGGTAGCATGGAGAAGTTGCCAAGAGTTCTTTAGGTGCCTC7380.11320598427081081No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG7380.11320598427081081No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG7380.11320598427081081No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG7380.11320598427081081No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA7270.11151863220173368No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC7150.10967788449001317No Hit
CAGTGCCAACTTCGCTGCCAACTTTGGTGCCATTGGTTTCTTCTGGGTTG7130.10937109320472643No Hit
CTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGG7010.10753034549300593No Hit
GATGCCAATAATGACCCACAGTGGTCTGAGGAACAGCTGATTGCTGCAAA6900.1058429934239288No Hit
GAAGTTCTGCGCTGGTCGGCGGAGTAGCAAGTGGCCATGGGGAGCCTCAG6880.10553620213864204No Hit
GTGGACCTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATG6880.10553620213864204No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG6870.10538280649599868No Hit
CCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTCTGAGCATTGCCAATG6860.10522941085335531No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT6860.10522941085335531No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT6820.10461582828278179No Hit
CTCCTCACAGCCCACTGGTCCTCAAGAGGTGCCACGTCTCCACACATCAG6820.10461582828278179No Hit
AAGGCGCCTATCAGGACCGCGACAAGCCAGCCCAGATCCGCTTCAGCAAC6800.10430903699749504No Hit
GGTGGTGTCTGAGGAGGTCTGCAGTAAGCTCTATGACCCGCTGTACCACC6770.10384885006956492No Hit
CACACTGTACTACCAACTAGGAGACCACGAACTGTCCCTCAGTGAAGTTC6760.10369545442692155No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG6750.10354205878427819No Hit
CGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCG6670.10231489364313118No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG6670.10231489364313118No Hit
CCGCCACCGCATCGCGCAGCTCTTTGCCGTCGGAGCGCTTGTTTGCTGCC6620.1015479154299143No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC6570.10078093721669742No Hit
GAAGGTTTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCAAC6570.10078093721669742No Hit
CATGCTGAAGCGACATTGTTGGTCAAGAAACCAGTTTCTGGCATAGCGCT6560.10062754157405406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTAAA2250.044.0105133
CGACGCA950.044.0105131
CCGATCA302.5218742E-644.0105132
ATCAATA302.5218742E-644.0105135
TATTGTA3250.044.0105134
AGTTAAA351.4420766E-744.0105131
AATAGCG351.4420766E-744.0105136
TGATCGT800.044.010516
GGGTGTA254.434455E-544.010516
CATATTC207.8447367E-444.010511
GTATTCT551.8189894E-1244.010516
GATAACC207.8447367E-444.010515
AAATATT502.7284841E-1144.010515
GCGAACG207.8447367E-444.010512
CGTGATA207.8447367E-444.010515
TTGCTAA254.434455E-544.010516
GTCCTAT254.434455E-544.010515
GAACTAA1300.044.010512
TAGCGAG1050.044.010513
GGTCCGT502.7284841E-1144.010513
Produced by FastQC (version 0.11.5)