FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005293227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1067762
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC165411.5491279891960943No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG141321.3235159145952No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC127341.1925878613398866No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG121901.1416401782419676No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC100430.9405654068977918No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG90960.8518752306225544No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC78160.7319983292156866No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG65170.6103420050535606No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC62580.5860856632845147No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG51000.477634529042989No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG50380.47182799163109385No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC50230.4704231841927321No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC50180.4699549150466115No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC43560.40795608010024703No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC38550.3610355116589652No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA35050.3282566714305248No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG34870.3265709025044907No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG33330.3121482128039769No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC33190.31083705919483934No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC32270.30222090690622067No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG31290.29304283164225736No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG30960.2899522552778615No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC30650.28704898657191397No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC30560.2862061021088969No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG28850.2701912973115732No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG28600.2678499515809703No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC27530.25782899185398994No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG26910.25202245444209476No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC26810.2510859161498536No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC26700.2500557240283884No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG26640.24949380105304367No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA26470.24790168595623369No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG25670.24040937961830444No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA25510.2389109183507186No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG25220.23619495730321927No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG25070.23479014986485755No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG24990.2340409192310646No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC24760.23188688115890993No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC24650.23085668903744466No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC24440.22888995862373826No Hit
CCAGCGCGTCTTCCCGGATGTTTCTTCTCGTGGGAGCTCCCAAAGCAAAC23780.22270880589494663No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC23070.21605938402003444No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT23050.21587207636158617No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG23020.21559111487391386No Hit
GCAGTGTTGCTCCATTCCTAGCTTGGGAAGCTTCCGCTTAGAGGTCCTGG22220.20809880853598461No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC21700.20322880941633062No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG21230.19882707944279715No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG21060.19723496434598722No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG20740.19423804181081553No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC20670.1935824650062467No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG20470.19170938842176438No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG20300.19011727332495446No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC20250.18964900417883387No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC20000.18730765844823097No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG19920.18655842781443804No Hit
CAACCCGCTGCAGGACGCGAACCTCTGCTCACGCGTGTTCTTCTGGTGGC19520.18281227464547342No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA19490.18253131315780108No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG19270.18047092891487054No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC19210.17990900593952583No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG19210.17990900593952583No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC18940.17738035255047474No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG18680.17494535299064773No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG18480.1730722764061654No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC18360.17194843045547603No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT17960.1682022772865114No Hit
CAACAGGGTCAGCTTCTGGAGGTTGGTTGGAACAATACGGCAAGTGCTCG17750.166235546872805No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG17730.16604823921435677No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG17720.16595458538513264No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG17050.1596797788271169No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG16910.1583686252179793No Hit
CAACCCAGCCCAGGAGGATAGCAACAGCGACGGAGTGGGAGACATCTGTG16660.1560272794873764No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG16520.1547161258782388No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG16520.1547161258782388No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG16470.15424785673211822No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC16230.15200016483073944No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG16210.1518128571722912No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC15860.14853497314944716No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG15860.14853497314944716No Hit
GGAGACGGACATCAGGACTCTCGGGACAACTGTCCCACGGTGCCTAACAG15700.14703651188186131No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC15640.14647458890651663No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC15600.14609997358962015No Hit
GCTGTCCTTTGGAGGGTGTCTTCTGGGTAGAGGGATGGGAAGGAAGGGAC15530.14544439678505133No Hit
CAAAGTCCGCCAAGGCACCCTGAAGAAGGCGCGCTACAATGCTCAGTGCC15500.145163435297379No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC15460.14478881998048254No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT14960.14010612851927678No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG14890.13945055171470797No Hit
CCAGCTGAGCAATGCATGGAGTGGACCTGTAGGCGACTTGCATCGTCTTC14770.13832670576401856No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG14740.13804574427634622No Hit
CACTGGCACTGGCACACGCTATGGCAAATGAAGTGCAAGACCTGCTCTCC14610.13682824449643272No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG14520.1359853600334157No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC14190.13289478366901988No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG14070.13177093771833048No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG14070.13177093771833048No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC13880.1299915149630723No Hit
GGCTGCGGAGCTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGG13830.1295232458169517No Hit
CCAGGCCAACCTTGGCTAAATGGAGCAGCGAAATCAACGAGAAACTGGAC13770.12896132284160702No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT13760.12886766901238292No Hit
GTGGACCTGAACACAGGTGAGCCAATGGTGGCAGCAGAAGTAGGCGTATG13750.12877401518315879No Hit
CTGATGAGGAAAGCATCCGGGCTCACGTGATGGCCTCCCACCATTCCAAG13750.12877401518315879No Hit
GCAAGTTCCAACCTGGAAAGGCCATCGGCTTTGTCAAGGATGTAGCCAAC13710.12839939986626234No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT13700.12830574603703823No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT13690.1282120922078141No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC13510.12652632328178No Hit
CCAGAGGGTGCAAGAATAAATGCCATTCACATTTCCACGATACACTCGGC13350.1250278620141942No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC13270.12427863138040125No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC13240.12399766989272891No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG12900.12081343969910897No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC12890.12071978586988487No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG12610.11809747865160963No Hit
GGAAACGTCTCCCAGATCGCTCCCTTGGCTTTGAGGCCGAAGCTGCTGTG12500.11706728653014435No Hit
GTTGCATGAACTTCGAAGGGCAGATATTACTGCACAAACTGCCATCTCGC12480.11687997887169611No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG12310.11528786377488617No Hit
GGCAAGAAGGCAGCTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTAC12050.11285286421505916No Hit
GGTGATGAGGAACACGGTGTATGCTGTGAAGAGGCTGAAGGAGAACGCTG12050.11285286421505916No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG12030.11266555655661092No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC11950.111916325922818No Hit
CCGCAGGATATACGCCAACATGTTCAAGAAGTTTGCAGAGCAGGATGCCA11920.11163536443514566No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG11910.11154171060592155No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC11680.1093876725337669No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG11610.10873209572919808No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG11520.10788921126618103No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG11440.10713998063238811No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG11370.1064844038278193No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG11310.10592248085247462No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG11160.10451767341411289No Hit
CTCAAGGACATCGAGACGGCCTGCAAGCTGCTCAACATCACCGCAGATCC11130.10423671192644055No Hit
CTACTTCATCAAGGTGCACGTCGGCGACGAGGACTTCGTACACCTGCGAG11100.1039557504387682No Hit
CCCATCCACGTATGCGGCGCTATATCAAGAACCCTCGCATTGTGCTGCTG11020.10320651980497526No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC10950.10255094300040647No Hit
CGGCGACAAGTACATCGCCAAGTTTCAGGGAGAAGACACGGTCGTGATCG10920.1022699815127341No Hit
CTGGCTTGACCACTGGATGCTTTGCCGAAAGCAGCTTGGCTTGGTAGCTC10730.10049055875747592No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG10710.10030325109902768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)