FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1323905
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGGAATGCTGCCATGGAGATCTGCTTGAATGTGCTGATGACAGGGCGG33594625.375385696103574No Hit
CGCCCGTGATACACCAAGAAATGATTGGTGGATTACGGAACAACAACGAG235301.777317858909816No Hit
CCAAGAGCATTTCGCTCACTTGCTGATCCTCAGGGACACCAAGACCTACA212591.6057798709121878No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG125130.945158451701595No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC123310.9314112417431764No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC113460.8570101329022852No Hit
CTGGTCTCTGGACACTATGGGCACACGACTCCTCCCAGCTCTGTTTCTTG110270.8329147484147277No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA100120.756247615954317No Hit
CCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTC98850.7466547826316843No Hit
GCCGAGAACATGTTGCTCACCTGCTGTTCCTTAGGGACACCAAGACCTTG92650.6998236278282807No Hit
TGTGACCGAACTCAACGGCGACATAATCACCAATACCATGACATTGGGTG82590.6238363024537259No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC76080.5746635899101521No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC68790.5195992159558276No Hit
CTGCAGAACCATGGAGCTTTGTGAAGAATGCCACCTCGTACCCTCCTATG63400.4788863249251268No Hit
CCAGGAATATGAACAAGCCGCTGCTTTGGATCTCTGTCCTCACCAGCCTC63090.47654476718495664No Hit
GCAATCCAGAGCGGCCATCAACAAATGGGTGTCCAATAAGACCGAAGGCC61230.4624954207439355No Hit
CAAAGTGGCATTCACAGGCTCCACTGAGATTGGCCGCGTAATCCAGGTTG58430.44134586696175326No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC57400.4335658525347363No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG55510.4192899037317632No Hit
GGAGTCGCTACCCACATAAGCCTGAAATCAACTCCACTACCCATCCTGGG50500.38144730928578713No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT48450.36596281455240365No Hit
CTGAAGAATGCTAGCCCATCTGGCTGCCGATCTGCTATCACCTGCAACTC48050.36294144972637765No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC48040.36286591560572695No Hit
TCCTGCTCGCCTGTTGGCTCCTGTGCCTGTGCCGGCTCCTGCAAATGCAA47380.35788066364278404No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG43080.32540099176300413No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC43030.3250233211597509No Hit
CGGTCGTGTGGTACTTATTCAGCGCCGGAAATGAGGCCGATGTACATGGA41610.31429747602735847No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC39800.3006258001895907No Hit
GACTATTTGTCGGAGCACGGATCTACCACACCATTGCATATTTGACACCC38840.29337452460712815No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC38790.29299685400387493No Hit
GAACCGCCCATTCCTGTTTGCTGTGTATGATCAAAGCGCCACTGCCCTGC38650.2919393763147658No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC36970.27924964404545644No Hit
GTTCCAGGAGCCGAATCAGAAATGTCATCCTCAGGCACGCCAGACTTACC36530.27592614273682775No Hit
CCATTACGGAGAGTTTCCTATGTTTCACTGTGCAAATATATCTGCTATTC36190.2733579826347057No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC35790.2703366178086796No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC35100.2651247634837847No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC33200.25077328056016107No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG32530.24571249447656743No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC32300.24397520970160244No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG31870.24072724251362446No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG30260.2285662490888697No Hit
CCCTAAGGGCACCGAAGTGTACCCTATGCTGGGCTCTGTGCTGAGAGACC30060.22705556667585663No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG30030.2268289643139047No Hit
CCACCAATGCCTGCTACGGTGGTACTGCCTCCCTCTTCAATGCTGCCAAC29900.22584702074544624No Hit
CCCAAATTATGTGGTCGCTGACCGGACCGAATGTTTCATCACTGGCTGGG28730.21700952862932008No Hit
AGACTCACAGAACACAGTTGAAGAAGGAAAGTGGCGATGGACCTCATCCC27890.21066466249466542No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC27650.20885184359904976No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG27490.20764329766863937No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC26720.20182717037853923No Hit
GGTCATCCGGACATCCCAACGCATGCTCCTGGAGCTCACAGCCTTCTGTG26320.19880580555251323No Hit
ATGTCAATATTTGGTGACATAACTAGAAAGTTCTGTAAAGATGTAGTTTC26240.198201532587308No Hit
GTTTGGAGATGTCCCAGAATAAGGCACAATGTCAATAGCAGGAGTTGCTG26190.19782386198405477No Hit
TGGAAACTTCGCTATCATGGAGATCCAATGCCCTGCCCTAAGGAAGACAC25900.1956333724851859No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG25500.19261200765915984No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC25400.1918566664526533No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA25280.1909502570048455No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG25120.1897417110744351No Hit
GTGTCATCATGTAGTGGCATGCTCAGCTCTGGATTTGCAGTCAGTTTGGG24820.18747568745491558No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG24220.1829436402158765No Hit
CATCCAGGAGTTCCAACACGACCAGGAGCTACAACGGATTCAGGAACGGG24100.18203723076806871No Hit
GAAGAAGGCTTGGAACCTGTCTGCTTTGAAAGGACTGATGACATCGGAGG23610.1783360588561868No Hit
GAATTGACAGGACTCCCAACTAGTACAATGACAGAAGATAAGGTCACTGG23560.17795838825293356No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG23420.17690091056382445No Hit
GGTCCACAAAGGAAATTCCCGATGAGATCAGCATTCTCCTTCTCGGTGTG23380.17659877408122182No Hit
CCTACACCTCTTGCATTCCATCCTCAACACCCAACTGTCTCGATGCAATG23140.17478595518560622No Hit
GCAGTTGCTGTAGGATTCTAAATCCAGGTGATTGTTTCAAACTGAGCATC22800.1722177950834841No Hit
CCTGAGGCAGAGTTGAGAATGGAGAGAATGTTACCTCTCCTGGCTCTGGG22540.17025390794656717No Hit
CCCTAAGGGAACGACACTCATCACCAACCTGTCATCGGTGCTGAAGGATG22190.16761021372379437No Hit
CTAGCTTCAGGTGTCACCATCACGTCCTGGAAGAATAAGGAGTGGAGCTC21640.1634558370880086No Hit
GAAAGTTCCATTTCCGTTCCAGAGGGAAGGGAACCTCCCTAGGTCCTTCC21220.16028340402068125No Hit
CAAAGCCTTCACTGTGTGCCTCCACTTCTACACGGAACTGTCCTCGACCC20310.153409799041472No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG20000.15106824130130184No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC19600.14804687647527578No Hit
GGGCTTGTCCTCCTTTCTGTTACGGTCCAGGGCAAGGTCTTTGAAAGGTG19560.14774473999267318No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG19490.14721600114811864No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC19340.14608298933835887No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC19030.1437414315981887No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC18490.13966258908305354No Hit
GCCCAGCGGCATCAGGCTTTCTTAAAGTGAGAGCACGCCTGTACTAGAGC18390.13890724787654704No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC17910.13528161008531578No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC17890.1351305418440145No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC17480.1320336428973378No Hit
GAACAGGACCAGAGACGGGTGGCAATGGTTCTGGAGCCCGAGCACCTTCC17430.13165597229408454No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG17340.1309761652082287No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG17140.12946548279521566No Hit
GGAGAAGTTGAGACAGGCGGTCAAGCAGGTGCTGAAGTGTGATGTGACTC16990.1283324709854559No Hit
GGCCAAGTGGGAGATCTTCCTCTTCCTGGCCATCCTGCTACAGCAACTGG16740.12644411796918964No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG16500.12463129907357401No Hit
GCTGCTCAACTCGCTCTGCACAGCAGTCAAAGCCATCTCTTCGGCGGTGC16040.12115672952364408No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG15810.11941944474867909No Hit
CCATTCCCACAGTTGACCTCCAGTCCCGCTGGGCAGCACAAGTAATAAAG15550.11745555761176218No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC15460.1167757505259063No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC15410.11639807992265307No Hit
GTGCAGGTTAAAGATTCGCTTGACCAGTTGGTAGGAGGAGTCCCAGTAAC15190.11473632926833875No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG15050.11367885157922963No Hit
CTTCGACACGGTCATCACCAACCAGGAAGAACCGTACCAGAACCACTCCG14920.11269690801077117No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT14780.11163943032166206No Hit
GGTAGCCGGACTCAGCTGCGAGGAGATGGGCTTCCTCAGGGCACTGACCC14710.1111106914771075No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT14640.11058195263255295No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC14380.10861806549563602No Hit
CCACTTGGGCTCCCTGTTTGGAGTCTGGCTGGCTCTGAGCATTGCCAATG14360.10846699725433472No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG14310.10808932665108145No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT14270.10778719016847885No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG14150.10688078072067105No Hit
GCACCAAGAACACCATACTGAAAGCCTACGATGGGCGTTTCAAGGACATC14100.1065031101174178No Hit
CACAAACCTTGCCAACAGCAGCCTGGGCTTCATTCTTGCTGATGCTGGTT13900.10499242770440477No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC13860.10469029122180218No Hit
CCAGACCATGGACAACCATATGCAGCTGGGCATGATCTGCTACAATCCAG13660.10317960880878915No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC13570.10249980172293328No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG13490.10189552875772807No Hit
GCAACGATCACATCGACACCTGGGAGTGGAATGATGTGACGCTCTACGGC13440.10151785815447484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAT750.044.0146266
CCTTAGC600.044.0146261
CGTATGT254.4344997E-544.0146265
GTATTCT1050.044.0146266
ACGCAAT207.844066E-444.0146262
CCCTAAC207.844066E-444.0146261
GTCCTAT408.289135E-944.0146265
TAGCGCA408.289135E-944.0146264
GTTAGTA502.7284841E-1144.0146261
AAGCGTC207.844066E-444.0146265
CGTTCGT502.7284841E-1144.0146266
CTAACTT207.844066E-444.0146263
ACGATAG207.844066E-444.0146262
TGCGCGG207.844066E-444.0146266
ATATACA750.044.0146261
CCATATA207.844066E-444.0146261
AACGATA207.844066E-444.0146261
GCTAGTC302.5222344E-644.0146266
GACGCGC254.4344997E-544.0146265
AATAGCG207.844066E-444.0146266
Produced by FastQC (version 0.11.5)