FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339020
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC194681.4538991202521248No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC177721.3272393242819376No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG177711.3271646427984645No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC129770.9691416110289616No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG128620.9605532404295678No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC123090.9192543800690057No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG106170.7928933100327105No Hit
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA104380.7795253244910457No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG89140.6657107436782124No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC88610.661752625054144No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC88340.6597362250003733No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC86330.6447252468223028No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC85380.637630505892369No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG75670.565114785440098No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG71020.5303878956251588No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC68360.5105226210213439No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG68200.5093277172857761No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC67560.5045481023435049No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG65240.4872219981777718No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC64870.4844587832892712No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC63310.4728084718674852No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC62160.46422010126809155No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG61410.4586189900076175No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC59560.4448029155651148No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC56280.4203073889859748No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG54990.41067347761795947No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG54480.40686472196083706No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG53270.3978282624606055No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT52450.39170438081582054No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG51680.38595390658840045No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC49630.37064420247643803No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG44750.33419963854162No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC44060.32904661618198383No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA41620.31082433421457484No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC41180.30753834894176335No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC40440.30201191916476233No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG40440.30201191916476233No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC40440.30201191916476233No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG37390.27923406670550105No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG36940.2758733999492166No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA35800.267359710833296No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG35500.2651192663291064No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG34740.2594434735851593No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG34410.2569789846305507No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC34400.2569043031470777No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG34340.2564562142462398No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG34120.25481322160983405No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC33830.2526474585891174No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC33490.2501082881510358No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG33470.24995892518408983No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC32010.23905542859703363No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG31230.23323027288614062No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG30610.22860002091081538No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC29740.22210273184866547No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG29720.2219533688817195No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG29220.21821929470807006No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC29100.21732311690639425No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG28040.20940687965825752No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG27380.20447790174904032No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC27130.20261086466221565No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG25980.19402249406282207No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG25130.1876745679676181No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG24920.18610625681468537No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG24870.18573284939732043No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT24560.1834177234096578No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC24260.18117727890546817No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG24090.1799076936864274No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG23970.17901151588475153No Hit
GGTGTTACACAGCCCAAGAGGACTGAGACTGGCACTTAGCCCAAGAGGTC23370.17453062687637227No Hit
GTGACGGAAAGATGTGGTGTGCGACCACAGCCAACTACGATGATGACCGC23000.1717674119878717No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT22900.17102059715314186No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG22860.1707218712192499No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC22620.1689295156158982No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG22590.16870547116547924No Hit
GGCGATGACCACTCAGAAGGAGGCCTGGTGGAGAACCACGTGGACAGCAC22520.16818270078116832No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA22510.16810801929769534No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG22200.16579289331003272No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT22030.16452330809099192No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC21820.16295499693805918No Hit
CGTCCGGAAGGACCGAGCTCTTCTCGCGGATCCAGTGTTCCGTTTCCAGC21820.16295499693805918No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT21380.15966901166524772No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC21370.15959433018177474No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG21240.1586234708966259No Hit
GAGGACCTCGGTCTTCATCCAAGTGGCCTGAGTATTTCACTGGCAGGTTG20730.15481471523950352No Hit
CGAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTG20370.15212618183447596No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC20260.15130468551627307No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG20100.15010978178070528No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG20090.1500351002972323No Hit
AGCGGGAGTATGCGGCTAACAAGTTCATCACCAAGGACAAGAGGCGCAAG19990.14928828546250242No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG19850.1482427446938806No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG19700.14712252244178578No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG19690.1470478409583128No Hit
CACTACAGGTATCTGTCCCTGGGCCAGCAGGGACCTCTGAAGCCTTCTTT19140.1429403593672985No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG18860.14084927783005483No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC18810.14047587041268988No Hit
CATAGGAGCGCAAAGAAGCTACAGCCTGGACTTACCACCACTAAACTGCG18720.13980373706143298No Hit
CACTTGGCACAGTAACGATGACGATGTGTATAGGGCGCCTCCAATTGACC18710.13972905557796No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC18460.1378620184911353No Hit
CTGTAGATGTCATGAGGCGAGCTGGGATTAAGGTCACCGTTGCAGGCCTG18460.1378620184911353No Hit
CATGGTGACGATGACACACCCTGGTGGCATGCCCGTGTATGTTGGTTTAG18390.1373392481068244No Hit
CTGGAGAGTCCAGGAGAGAAGACTCACCTCTGTCGCTTGGGTTAAACAAG18340.13696584068945947No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC18250.13629370733820256No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG18160.1356215739869457No Hit
GCTCTCCAATTTCGGTTACCCTGGCTACCAGTCCAAACACTACGCCTGCA18030.13465071470179685No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT18030.13465071470179685No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC17820.13308240354886408No Hit
GGTCCACAAAGGAAATTCCCGATGAGATCAGCATTCTCCTTCTCGGTGTG17670.13196218129676926No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG17640.13173813684635033No Hit
CAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGC17510.13076727756120146No Hit
CGCCCGTGATACACCAAGAAATGATTGGTGGATTACGGAACAACAACGAG17480.13054323311078253No Hit
CCTACATGTTGAAGGGCCTAACCAGCCTTGGGAGTATTGACTGGTCCCTT17290.12912428492479575No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG17200.12845215157353884No Hit
GGAATGTTAAACTCCCGCGTCATAGAAATAATGCAGAAGCCCAGATGTGG16930.12643575151976819No Hit
GCTGCTAGACGTGAAGCACTTCTCGCCGGAGGAAATTGCTGTCAAGGTGG16650.12434466998252454No Hit
GCAAGGGCACAAGTCCTGGATGTTGTGTGTATCGAGAGGCCAAAGGCTGG16400.12247763289569985No Hit
ATATACAACCTGCTTGGGTGGAGAAGCCATTGTCTTCGGAAACCTTGGTG16310.12180549954444295No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG16080.12008782542456424No Hit
GCAAGTTCCAACCTGGAAAGGCCATCGGCTTTGTCAAGGATGTAGCCAAC16060.11993846245761826No Hit
CTCCCTGCATCTTCGGTGGTACGGTGTACCGCAGCGGAGAGTCCTTCCAG16050.11986378097414527No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG15900.11874355872205046No Hit
GCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAG15660.11695120311869873No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT15420.11515884751534704No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG15340.11456139564756315No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG15340.11456139564756315No Hit
GCACTCGTTCTCGATCTCGGTCGACCTCCAAGTCCAGATCCGCACGAAGG15250.11388926229630625No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG15250.11388926229630625No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC15120.11291840301115741No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG15090.11269435856073845No Hit
GCCTCAACCTGGAGGCCATCAACTACATGGCAGCTGACGGTGACTTCAAG15030.11224626965990053No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA15030.11224626965990053No Hit
GATGAACGACATCAACGCGCCCTATCTGGTCATGGGACAGAAACAGGGTG14650.10940837328792699No Hit
GTTAGTAATGCTCTGACCAAGCCGAGATGCCCATTCTCTTAGTGATGGCG14630.10925901032098101No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC14630.10925901032098101No Hit
GGACAGATGAAGCTGCTTTCCAGAAGCTGATGAGCAACTTGGACAGCAAC14620.10918432883750802No Hit
GACGGCTATGATTACGATGGGTACCGTCTGCGGGTGGAGTTTCCTCGAAG14590.10896028438708906No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC14470.10806410658541321No Hit
GTGTGCATATCAAGCTGCCCAACCTGGGCAAGCATGAGAAGTTCTCGGAG14410.1076160176845753No Hit
CCCACTCAGTGAAATGACCCGCGAAGAGATCAATGCGCTAGTGCAGGAGC14330.10701856581679138No Hit
GGCTGCGGAGCTTGCAGAATACACTGCCAAGATTGCCCTCCTGGAAGAGG14260.10649579543248047No Hit
CAAGGTCTCCTGATCCGTCCGAGGCTGCTTCCCAGAGGAGCAGCTCTCCC14140.10559961763080461No Hit
CTGAACACCACCCAGTGTGGAGCAGCCCAGCCAAGCACTGTCAGGAATCC14050.10492748427954773No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC13950.10418066944481785No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC13920.1039566249943989No Hit
GAAGGTTTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCAAC13870.10358321757703394No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG13740.10261235829188511No Hit
GCAGTGTTGCTCCATTCCTAGCTTGGGAAGCTTCCGCTTAGAGGTCCTGG13720.10246299532493913No Hit
GTGGGAACGTCTTACGGTAGCTGATGCATTGGAACCAGTGCAGTTTGAAG13710.10238831384146614No Hit
GAGGAAGCCAAGGGCTTAGACCGAATTAATGAGAGGATGCCGCCTCGCAG13600.10156681752326328No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG13600.10156681752326328No Hit
CCATTCGTTCGTGAGTGTCCCGTGCACGTCCTGACCCTGGACAGTGAAGG13580.1014174545563173No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG13520.10096936565547937No Hit
CTTCTCTCACGCTGTGTCATCCAACGGGAATGCAGTTGAGGATCCAATGG13470.10059595823811444No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAA650.044.0107965
CTATGCG408.296411E-944.0107963
CCTTAGC207.8474625E-444.0107961
TCGCACC254.4368047E-544.0107964
CGTATGT750.044.0107965
CATATAC502.7284841E-1144.0107962
CGTATAT502.7284841E-1144.0107964
GATAACG454.783942E-1044.0107961
ATAGGAC254.4368047E-544.0107965
GCGCGTC408.296411E-944.0107964
GCGCGAT207.8474625E-444.0107961
CGTGATA2250.044.0107965
TTGCTAA254.4368047E-544.0107966
TTTCGCG1550.044.0107966
TAGCGCA900.044.0107964
GTTAGTA1200.044.0107961
CACGAAA3550.044.0107964
TTTCATG502.7284841E-1144.0107966
ATCGTTG408.296411E-944.0107966
TACATTT207.8474625E-444.0107965
Produced by FastQC (version 0.11.5)