FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610202
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCAACTTTACGTGCACTGAGCTGCAACTTCTTACTTCCCTACTGA353352.1944451689912197No Hit
CATGGATGACCAGCGCGACCTTATCTCCAACAATGAGCAACTGCCCATGC337272.0945819220197217No Hit
CCATGTCTTACTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCC321041.9937871149085642No Hit
GAGAAGAACACCCTGCCGACCAAAGAGACCATTGAGCAGGAGAAGCGGAG215651.339272960783802No Hit
GACTGAGAGGCAAGAGTTGTTCCTGCCCTTCCCTTTGTGACTTGAAGAAC205131.2739395429890161No Hit
GGTTTGGCAGTGAAGGAACTGAAATGAACCAGACACACTGATTGGAACTG159890.9929810048677122No Hit
GCCCATGTCTCATGAACTACCCTGTGGCCATACTCAGACAGTCCAGGCAC135060.8387767497494104No Hit
CCAATGATCAGGGAAACCGAACCACTCCAAGCTATGTCGCCTTTACGGAC130430.8100225934385872No Hit
GCTACGGACGACCTCGGCAGATCATCAGTTAGAGCGGAGAGGGCTAGCCC126470.7854294057515766No Hit
CGCAAGTTCACCCTCAGAAGGTGACCAAGTTCATGCTGTGTGCTGGACGC122620.7615193621669828No Hit
GGTAGAGCTGTGCATTAAACTTGCACATGACTGGAACGAAGTATGAGTGC115880.7196612598916161No Hit
GCTCCACTCAGTGTCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAG93950.5834671674733978No Hit
GGGTAAGTAAGGAGGTCTCTGTACCATGGCTCGTACAAAGCAGACTGCCC92770.576138894374743No Hit
GGAAGGTGAAGGTCGGAGTCAACGGATTTGGTCGTATTGGGCGCCTGGTC89060.5530983069204982No Hit
CCCAAGCGAAATTGTGGGCAAGAGAATCCGCGTCAAACTAGATGGCAGCC87460.5431616654307969No Hit
CGGATACTTCCACAGGTCCCACAACCGCAGCGAGGAGTTTCTCATTGCTG78450.4872059530419165No Hit
GGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTACTCAGAGTATCGC70900.44031742601238855No Hit
CCGGTCTCTAGTAGTGCAGCTTCGGCTGGTGTCATCGGTGTCCTTCCTCC67140.4169663185115905No Hit
GGTTCTAAAGGAAAGGGTGGAGAGATTCAACCAGTTAGCGTGAAAGTTGG66020.41001066946879955No Hit
GGCTCGAGATCTTCGCGGGAAGAAGAAGGAGGAGCTGCTGAAACAGCTGG63780.39609937138321777No Hit
GGAGTCAAGGATGGTGGCCGGAGAGACTCTGCAAGCTATCGGGATCGTAG62930.39082053059181393No Hit
GCGCACCATCTCTGTGATCCTGTTCCTCAACAAGCAAGATCTGCTCGCTG62070.38547958579109953No Hit
CTGGTTCGGCGAGAGCATGACCGATGGATTCCAGTTCGAGTATGGCGGCC62030.38523116975385696No Hit
CAGAGAGCCTCCACTAGAGTGATGCTAAGTGGAAATGTGAGGTGCAGCTG59220.36777994313756907No Hit
GAGCCCGAAGAAGAACCTGAAGAGACAGCAGAAGACACAACAGAAGACAC58540.363556870504446No Hit
GAGATTGCCCACAATGCTGCCTTTGGCAACTGCAAAGCAATGTGGAATCG57520.35722226155476144No Hit
GGAATGGACATGCATTGCCTACTCGCAGCTTCGAGATCAGTGCATTGTTG56870.3531855009495703No Hit
CACGTTCTGGCATCACTTGGCCTTTCTGGATGCTGGACACCTGAAGCTTG56690.35206762878197895No Hit
GTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGTGGACGTGGTT55880.3470372040278176No Hit
GACAAATGCGACGAACCTCTGAGCATCCTGGTGAGGAATAACAAGGGCCG54200.3366037304636313No Hit
CTTTGCCGATCCGCCGCCCGTCCACACCCGCCGCCAGCTCACCATGGATG53970.3351753382494867No Hit
GGAGACTTGGGCAATGTGACTGCTGACAAAGATGGTGTGGCCGATGTGTC52000.322940848415292No Hit
GCATATTGTACTGGCCTGCTGCTGGCCCGCAGGCTTCTCAATAGGTTTGG51290.318531463754237No Hit
CGGTGGAAGGAGGAATGAAATGTGTGAAGTTCTTGCTCTACGTCCTCCTG50590.31418418310249274No Hit
GGGCTTGTCCTCCTTTCTGTTACGGTCCAGGGCAAGGTCTTTGAAAGGTG50540.31387366305593956No Hit
CATTTGCTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTC50500.31362524701869704No Hit
CGAAGCTGCAGGATGCTGAAATTGCAAGGCTGATGGAAGACTTGGACCGG50200.31176212673937803No Hit
GCTGGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTG50050.3108305665997185No Hit
TGGGACAAGCCCGAGCATATCCCTGACCCTGATGCTAAGAAGCCCGAGGA49210.30561382981762536No Hit
GTCGTGTTCGGGTCTCTGGTCAGGGCCTTCACGAAGGCCACACCTTTGAG47210.29319302795549873No Hit
GCTTCGGTTACCCACAGTCTTTCGCCAGATGAGACCGGTGTCCAGGGTAC43790.27195345677126226No Hit
CCCTGGAGGGTATCCGCCAGTTCTACATCAACGTGGAACGAGAGGAGTGG42470.2637557275422587No Hit
GTGCAGTGGCAAGTCACCACTTACTGCAGCTTTAAGCTCCTGGGCTCAAG40460.25127282167082143No Hit
CGAAGGGACAGCGAAAGATGAGGGTGGCAGGAAGAGAAGGGCGCTTTCTG40400.25090019761495763No Hit
GGTGGACAGATTCTAGTGCTGAGAAGAAACACGTTTGGTTTGGAGAGTCC40040.24866445327977482No Hit
GACATGCGCATCATGGCGTGGCAAGAGCCTTCGGGTTCAGAAGATCAGGC39760.24692554101907713No Hit
GATGAGGCGAGAGGAGGAGGAGGAGGAGGAGAAGGCTCTGGGCTCCTCTG39090.2427645723952647No Hit
GACGGCAGATCCGAGTAGACCAGGCAGGCAAGTCGTCAGACAACCGATCC38930.24177090824629457No Hit
GCCCACTTCCCGTTTCCTCTTTAACTGAGGAGAAGCTGATCCAGTTTCCG38880.2414603881997414No Hit
GGAAACCTTCCACCCAGTGCTGAATTTCCCTCTCTCATACCCTCCCTCCC38340.23810677169696723No Hit
GATTGTGGAGGAGGGCGATATGAAAGGTGAGGTCTATCCATTTGGCATCG38340.23810677169696723No Hit
CTGACAGGTGAATTGGATGGTTCTCAGAACCATTTCACCCAGACAGCCTG37630.23369738703591225No Hit
CTCGTCAGGATCCCGCTGCACAAGTTCACGTCCATCCGCCGGACCATGTC37310.231710058737972No Hit
CGCCCGTGATACACCAAGAAATGATTGGTGGATTACGGAACAACAACGAG36110.22425757762069604No Hit
GCTACTTGTGGACCTCACCAGAGACCAGGAGGGTTTGGTTAGCTCACAGG35570.22090396111792188No Hit
GATGCTGCTGACAAACCAAGAGGATTTGCCAGAGGTCTTGATCCTGAAAG35520.22059344107136866No Hit
CGAGGGAATGCAGAAAGAGTTAAGGAAGGCAGGTTGTCCTTCTATTCAGG35420.21997240097826237No Hit
CTGAGTATGCCAAAGCCGCTGGGAAGCTGAAGGCAGAAGGTTCCGAGATC34960.21711561654997324No Hit
GCAGAAGCCTCTGACTTTCAACGACCTAGTGAAACCAGTGTGTCTGCCCA34200.2123957118423651No Hit
GCAGTGTTGCTCCATTCCTAGCTTGGGAAGCTTCCGCTTAGAGGTCCTGG34200.2123957118423651No Hit
GGCACCTGGATTGAGTTGCACAGCTTTGCTTTATCCGGGCTTGTGTGCAG34100.21177467174925876No Hit
GGCGACATAAATACAGAACGGCCCGGGATGTTGGACTTCACGGGCAAGGC33140.20581268685543802No Hit
GCTCTCTGACCTCTTCCGGGACCCGGACCACTGTCGCCAGGCCTACTCTG33000.20494323072508913No Hit
CTTCGACACGGTCATCACCAACCAGGAAGAACCGTACCAGAACCACTCCG32990.20488112671577852No Hit
CCTACGTGAACAACCTGAACGTCACCGAGGAGAAGTACCAGGAGGCGTTG32780.2035769425202552No Hit
GTTCACGAAATCCTGTGCAAGCTCAGCTTGGAGGGTGATCACTCTACACC32150.19966438993368535No Hit
CGGGCAAGGATAGTTACTGACCGGGAAACTGGGTCCTCCAAAGGGTTTGG31810.19755285361712382No Hit
GAAGAGAACTTGCAAAGGTAGAAGAAGAAATCCAGACTCTGTCTCAAGTG30930.1920877007977881No Hit
CAGGGATACTCGGGCCTTTCTGTGAAAGTTGTAAACTCCACCACAGGGCC30160.18730569208086936No Hit
CGACGCAACCATGGCTGAAGAACAACCGCAGGTCGAATTGTTCGTGAAGG30140.1871814840622481No Hit
CACGGAGATCTCGCCGGCTTTACGTTCACCTCGGTGTCTGCAGCACCCTC29810.1851320517549972No Hit
CAAGGAGTAGGGCACAGGGACATCTTTCTTTGAGTGACCGTCTGTGCAGG29630.18401417958740582No Hit
GGCTCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTGGCT28930.17966689893566148No Hit
GTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGG28600.1776174666284106No Hit
CCGCCCTGCAATTAACGTTGGTCTGTCTGTATCTCGTGTCGGATCCGCTG28460.17674801049806174No Hit
CGTGCTGATCGTGATCTTCACAGTGCTCCTGCAGTCTCTCTGTGTGGCTG28420.1764995944608192No Hit
GTGCATTCGTCGCTGAACAGAAGGTGGAGTATACCCTGGGAGAGGAGTCG28400.17637538644219794No Hit
GATGGTCTGGTAGAAACACCAACAGGATACATTGAAAGCCTGCCTAGGGT27990.17382912206046197No Hit
GAGATTGACGCCCTGAAGGGCACTAACGATTCCCTGATGAGGCAGATGCG27070.16811555320388374No Hit
TTACTTGTGACTGGACCTCTGGTCCTCAATCGAGTTCCTCTACGAAGAAC27070.16811555320388374No Hit
CAAGGCATACGAACAGGCACTGCAATACTCGCCTTACGGCTCTACGTTGC26910.1671218890549136No Hit
CGCACACAGTACGACACGAGGAGAAAGTGCCATGTCACGTGTGTGGCAAG26750.16612822490594348No Hit
CCGGAGCTGGGTATCTCAAATCTGCTTTCATCCAGCCACTGCCCAAAGCC26600.16519666476628397No Hit
GACAAGACTATGTGCTCCGCTGCCCACCTTCAGCCTGCAATGAGGGACTG26320.16345775250558625No Hit
GAGCCTCCAGAACCGAAGAAGAGCCGCAGGTGTGTGCTGCTATGAACATC26110.16215356831006295No Hit
GATCTCTCTCATCTCGCTCGGCTGCGGGAAATCGGGCTGAAGCGACTGAG26060.16184304826350981No Hit
GACAACACATCTCAAGAAACTCAAAGAATCATACTGTCAAAGACAGGGTG26020.16159463222626724No Hit
CCAGACCTGGACCCGTGAAGTCTTCAGCTCCTGCAGCTCTGAAGTGGTTC25990.16140832019833534No Hit
TCCTGGTATTCTGGGTCTCCCTGGCTCGAGAGGTGAACGTGGTCTACCAG25920.16097359213316093No Hit
GATGTTACGGGAATTTCAATGGCTTCGAGCAAATAGTAGTGTTGTCAGTG25910.1609114881238503No Hit
AGCGCACTAAATCGTCTCCAAGTTCGAAGCATTCAGCAAACAATGGCAAG25830.16041465604936525No Hit
GCTGGCTTTCTGTTGACTGGCCCGGAGCTGTACTGCAAGACCCTTGTGAG25620.15911047185384194No Hit
CGTCTTGGCCAACATGATTAGTGAACCAAGGATCAGTTACGGAAACGATG25460.1581168077048718No Hit
TACTCTCACAGGTAGAATGAACTGTGTACTGGCCACATATGGAAGCATTG25300.15712314355590168No Hit
GCCATGCTTGAGGAAGAAGATACTGAAGATTAGACATGGTTGGGAAGACG24890.15457687917416574No Hit
GCAAGTTCCAACCTGGAAAGGCCATCGGCTTTGTCAAGGATGTAGCCAAC23920.14855279027103432No Hit
TTCTCTTGCCATTTGTAGCTTTGCCCATTGTCTTATTGGCACATGGGTGG23810.14786964616861736No Hit
CAAACACATTGTGCTTTCTGGAGGGTCTACTATGTATCCTGGCCTGCCAT23760.1475591261220642No Hit
CTTCGCAAATACTGTGGACAATGCCCGCATCGTTCTGCAGATTGACAATG23640.1468138780103366No Hit
CCTCTCCCAGGTATGCGACGGGAAAGTATCCGTCCACGTCATCGAGGGTG23630.14675177400102596No Hit
CATTGACACATGTCGCAGCATGGTGGCCGTGATGGATAGCGACACCACAG23600.14656546197309406No Hit
CCAAGGACATCTGGAATGTCACTGGTGCCCAGGTGTACTTGAGCTGTGAG23590.14650335796378341No Hit
GCACGTGTTCCCGACATAACATTGTACTGTAATGGAGTGAGCGTAGCAGC23530.14613073390791964No Hit
GCGAGAGGGCTTGAGTCTCAAGGACTCACTGATTACATACCCAAAGGCTC23270.14451602966584318No Hit
CAAGAAACGGCTTTCAGTTGAGCTGACCAGCTCTCTCTTCAGAACAGAGC23200.14408130160066873No Hit
CTCTAACAGCCCTCCTCTGCACTCCCACCTGACACACCAGGCGCTAATTC22750.14128662118169025No Hit
CTGCCTTGCATAAGGCCATAATGGTTAAAGGTGTGGATGAAGCAACCATC22740.1412245171723796No Hit
GTCTGACGTACAGAAGCGTGTGCAAGTGGATGTCAAGATGGCCATCCTGC22140.13749827661374164No Hit
TGTCCCACTGGTCCAGACATCATTCCAATTGTAGCTGGTGTGGTTGCTGG21990.13656671647408214No Hit
GATGAACATCATCCCAGAGGTGGTGTTCAGTGTCAGACCTCTTAATGGGC21680.1346414921854525No Hit
CTACGCCAACAAGTACAGCTTCTGGCTGACCACCATTCCCGAGCAGAGCT21610.13420676412027807No Hit
GACAGGGTACAAGCGGGAGCACATTAACCTGGGCTGCGACATGGATTTCG21120.13116366766405704No Hit
GGCAAGAAGGCAGCTCTTGATGAAGCTCAAGGAGTGGGCCTCGATTCTAC20360.12644376295644896No Hit
GCAACGAGACAATGACTGGGTGGGTGCATGATGTGTTGGGCCGGAACTGG20360.12644376295644896No Hit
CCTGTTAGCAGGTGGTAATACCATGGGTGCTGTGACACTAACAGTCATTG20170.12526378677954692No Hit
GGAACTAAACGTCACCAAGGTGGCTTCTCCTTGGCTGAGAGATGGAAGGC20090.12476695470506184No Hit
CGTCTCTCAGGATGGCGAGTAGCAGCGGCTCCAAGGCTGAATTCATTGTC19860.12333856249091728No Hit
CAGATGGAAACAAGTCACCTGCTCCCAAACCTTCTGACTTGAGACCAGGA19800.12296593843505348No Hit
GATGGGACAACACCCGAAAGGATAGCACAAATAACAGGACCTCCAGACCG19570.12153754622090893No Hit
CAACAAGACGATCCACGCAGAGCTCTCAAAGCTGGTGAAGAAGCATGCGG19420.12060598608124944No Hit
CAACCCAGCCCAGGAGGATAGCAACAGCGACGGAGTGGGAGACATCTGTG19130.11880496981124108No Hit
GGCTGGAACAACTCCACCTTCGCCTAAGAGCCGCAGGGACCCACGCTGTC19000.11799761769020285No Hit
GAGATGAGGCCAGACCATGGACACTACACCCAGCAATAGAGACGGGACTG18460.11464400118742865No Hit
CGAGAGGGACGCGTACAAGGAGAAATACGAGAAGTTGGTGAGCAGCGGCT18410.11433348114087548No Hit
CCCGAGTGCACGTGTCTGAGGAGGGCACTGAGCCCGAGGCGATGCTCCAG18360.11402296109432233No Hit
AAAGTTCTTTGTGAGCTGGCTGATCTGCAAGACAAAGAAGTTGGAGATGG18350.1139608570850117No Hit
GCTCCACTCAGTGCTAAGGCACCCTGCTTCCTTTGCTTGCATCCCACAGG18320.1137745450570798No Hit
CAGGCTCACAGACGCCAAACGCATTAACTGGCGAACAGTGCTGAGTGGCG18250.11333981699190537No Hit
GCTGCAGATTCGTTCAGAGTCTAAATTCCGTTAACAACTTGGAAGCTACC18070.11222194482431397No Hit
GAAGGTTTAAGGCTCAGAAAGGTAGCACATTCGGATAAACCTGGATCAAC18010.11184932076845017No Hit
GGACAGATGAAGCTGCTTTCCAGAAGCTGATGAGCAACTTGGACAGCAAC17920.11129038468465446No Hit
GAACAGACAAGGGAACAGCCTGGACATGGCATCAGAGATCCACATGACAG17820.11066934459154813No Hit
GGAACATCAAGGCCACCGATCTGCGGCCCATCTACATCTCTGTGCAGCCG17780.1104209285543056No Hit
GATCGAGACACGTGATGGGAAGCTGGTGTCTGAGTCCTCTGACGTCCTGC17650.10961357643326738No Hit
GTGAGAATTCAGCATGGAATGTCTCTACTATTTCCTGGGATTTCTGCTCC17530.10886832832153978No Hit
CAGAGTATCTGCCAAGCCCGGGCTTCCGTGATGGTCTACGATGACACCAG17370.10787466417256965No Hit
GCCAGCAACGCTTGTAGAACTCACTCTGGGCTGTAACGTGGCACTGGTAG17140.10644627195842508No Hit
CGGAACCGGTCAAACACACCCATCCTGGTAGACGGCAAGGATGTGATGCC17090.10613575191187194No Hit
GATCACCTGACTCCAGCTAGATTGCCTCTCCTGGACATGGCAATGATGAG16990.10551471181876561No Hit
ACTCGTCATTTGCATACGACAGTAGCATTCTGACCACACTTGTACGCTGT16840.1045831516791061No Hit
GGGTGTTTCGCGAGTGTGAGAGGGAAGCGCCGCGGCCTGTATTTCTAGAC16640.10334107149289344No Hit
GCAGTGGGAAAGTAATGGTCAAGTAACACTAGGTAGACTATCATGCCTGT16630.1032789674835828No Hit
CTAGCTCAGGTGTGTCGGTACTGAGCCTGTGTGAGAAAGGTGATGCCATG16560.10284423941840837No Hit
GGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTATGAGCAC16530.10265792739047647No Hit
GGTGACCAATGATGGTCACCAAATCAGCTGCTACTACTCCTGTAGGAAGG16480.10234740734392331No Hit
GCACCAAGAACACCATACTGAAAGCCTACGATGGGCGTTTCAAGGACATC16450.10216109531599141No Hit
CATTGGCCTTGCCATCCATGGAGCTGAACGAATTGTACGTGGGCAGACCG16230.10079480711115749No Hit
ATGCAGTTTGTTGAGCCAAGTCGGCAGTTTGTAAAGGACTCCATTCGGCT16220.10073270310184686No Hit
GAACGAGAGCAAGTAATTGCAGCCCTGAGAGCCAGTTTCAACAACCCTGA16200.10060849508322559No Hit
GAGCCAATGGAAATCTTCAGAAACTGTGTAAAGATTGAAGAAATCATGCC16170.10042218305529368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAA302.5247919E-644.0090142
GCCGGTT207.849504E-444.0090142
AGGTTAG1300.044.0090144
TTTATTA302.5247919E-644.0090143
ACGGTAT408.301868E-944.0090143
AGCGTAA254.4382716E-544.00900743
CGGCGGT351.4442412E-744.00900742
ACGCCGA254.4382716E-544.00900742
GCGGTTA254.4382716E-544.00900742
CCGGACG254.4382716E-544.00900743
GTTCGTT254.4382716E-544.00900744
TCGTCTA502.7284841E-1144.00900742
CAAGCGT254.4382716E-544.00900744
TTATTAT351.4442412E-744.00900744
GCGTAAG254.4382716E-544.00900744
TCGCAAG454.783942E-1044.0076441
CTTGCGA254.4390858E-544.0076440
TAGGCGT1250.044.0076441
GACGCCG254.4390858E-544.0076441
GTGTAAG551.8189894E-1244.00763741
Produced by FastQC (version 0.11.5)