FastQCFastQC Report
Thu 12 May 2022
EGAF00005293330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214108387
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTC8348020.38989691702268536No Hit
CGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC7883830.36821677611349246No Hit
CGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAG5922270.2766014952977998No Hit
CCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAA5700750.26625533356617176No Hit
TCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA5536100.258565303189174No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG5361230.25039794447659824No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT5260150.2456769710754021No Hit
GCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC4891160.2284431763058399No Hit
CACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTC4887380.22826663020911928No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC4754900.2220791098669105No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG4399590.2054842438283373No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG4155890.19410215817468188No Hit
CAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCC4151150.1938807749740322No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGT4034750.18844427612263503No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACC3879090.18117412654180615No Hit
GATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATA3868650.18068652303657773No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT3770880.17612014423330366No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC3753220.17529532834227554No Hit
CGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACA3733600.1743789700307256No Hit
TTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAAC3699230.17277370829943248No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT3663260.17109371806159093No Hit
GTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATA3660690.17097368539794752No Hit
TTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGG3605250.16838434264604496No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT3588490.16760156153995032No Hit
CACGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCA3545800.16560771157460544No Hit
CTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAA3501550.16354100131537583No Hit
CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG3492230.16310570776473132No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG3464200.16179655774063628No Hit
GCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAAC3403290.15895173690697134No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA3400040.15879994462804486No Hit
CCTCGATGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTT3388910.15828011445436746No Hit
CCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC3347710.15635585541074576No Hit
GGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT3312780.15472443870216068No Hit
GTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGA3242270.15143124682920525No Hit
GGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCT3236840.15117763696010658No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT3234930.1510884298054144No Hit
CAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACT3184910.14875222986944459No Hit
GTTAAATCAACAAAACTGCTCGCCAGAACACTACGAGCCACAGCTTAAAACTCAAAGGACCTGGCGGTGCTTCAT3151900.1472104873687176No Hit
GTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCA3148800.14706570088728005No Hit
CACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAACACACAATAGC3144360.1468583292816082No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT3058720.1428584859686043No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAAT3057940.14282205582166194No Hit
GAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT3046760.1422998903821549No Hit
CACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTT3025160.14129105554375132No Hit
CCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCC2999020.14007017856801657No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA2983390.13934017446967176No Hit
TCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGA2923480.13654205895259955No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACTCCCATGTCCCTTGGGAAGGTCTG2858730.1335178897032184No Hit
CTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACCCAAGTCAAT2792200.13041058499030214No Hit
CGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCT2734290.12770588010641545No Hit
TGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAA2721000.12708516644889767No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2679340.12513942295964334No Hit
GTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCA2639710.12328849126307229No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT2603520.12159822585558033No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATAT2601740.12151509039204522No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT2586210.12078975682535967No Hit
CTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTGT2505570.11702344009531958No Hit
GTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAA2504890.1169916804800365No Hit
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2486790.11614631424970755No Hit
CAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTTC2468140.1152752600952526No Hit
GATTAAAGTCCTACGTGATCTGAGTTCAGACCGGAGTAATCCAGGTCGGT2451530.11449948478664687No Hit
CTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGG2445780.1142309292162385No Hit
CACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAG2441560.11403383278021707No Hit
TGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC2398770.1120353122832129No Hit
TTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAAGAGAAATAAG2397380.11197039189314896No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA2382320.11126700982526201No Hit
CCGGAGTAATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACG2365020.11045900784820728No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGC2357760.11011992724974384No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACC2355990.11003725883937465No Hit
CTGGGATTAGATACCCCACTATGCTTAGCCCTAAACCTCAACAGTTAAATCAACAAAACTGCTCGCCAGAACACT2344130.10948333378458454No Hit
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2341670.10936843870576635No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGT2335400.10907559637072975No Hit
CCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAAC2304710.10764221020449798No Hit
CAACAATAGGGTTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTG2259230.10551805240585926No Hit
GCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCC2217960.10359052399007611No Hit
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT2201850.10283810133976676No Hit
ATTGAAACCTGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAGCCAAGCATAATATAGCAAGG2182090.10191520428389383No Hit
TGTTAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTA2176940.10167467190344112No Hit
GGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGC2176010.10163123595900987No Hit
CTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGA2167170.10121836096032986No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA2164040.10107217331939454No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA2163940.10106750278773527No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAA2154580.10063034102442703No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT2152700.10054253502923265No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)