FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18906876
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT686150.3629102978196927No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG304240.1609150025630887No Hit
CGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCG233680.12359524651243284No Hit
ATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGCCGGGG208590.1103249421004295No Hit
CTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAACAGCAGTTGAACAT195650.10348087119204674No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATGGC214750.040.7917870
TAGCATA61800.038.8031469
TCACCCG139650.038.6766433
CGGACAC141450.038.2447913
ACACGGA143100.037.89998216
ACCCGGC143000.037.820345
CACCCGG144000.037.483634
CCGGACA150050.036.30697312
GCCCGGA152950.035.594510
CCCGGAC152400.035.54236611
CGGACAG154700.035.39327219
ACGGACA155400.035.2111718
CACGGAC160500.034.02861817
CTCACCC160100.033.980882
GACACGG164900.032.99524315
GGACACG172100.031.65487114
CCTCACC175100.031.5220431
GTAGCAT76550.031.2803678
CCAGTAG77700.030.6779255
TCTCGAT180050.029.97076248
Produced by FastQC (version 0.11.5)