FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16768708
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11300.031.70804448
ATAATAC44500.029.19348117
AATAATA46200.027.89370716
GATCGGA13750.027.8051151
TAATACA47450.027.3784218
TACGACA13700.023.3269
GAAAATA56400.022.97227713
TATTGAA60150.022.8682339
GATTTAT61400.022.4644035
GCACTAC56900.022.28283525
TCGTATG18550.021.55403745
GTTACGA7700.021.2070314
GCGCGTT8150.021.02193862
GCTAGCG17600.020.18869454
AGCACTA65600.019.803824
CACTACA68450.018.9798126
AAATAAT69650.018.90133315
AGTCACG21300.018.7548928
AGATTTA64900.018.6832944
ACACGCG27150.018.46689852
Produced by FastQC (version 0.11.5)