FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293630

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293630
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16768708
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGAT44950.018.37748148
TAGCTCT47100.018.18410540
ATAGCTC47250.018.1256239
TTCTCGA46950.018.10827447
ACACGGA48850.017.80847416
TAGCATA18100.017.807279
CTCGATT47200.017.50467949
ATTCCGT49650.017.28640253
CGGACAC50650.016.90173113
ACGGACA55250.016.37256618
TTGATAG54350.016.00948136
GCGTTCG33050.015.6257720
CGGACAG58500.015.22618119
TTCCGTG57750.015.16647454
TGATAGC58600.015.14516437
ACCCGGC57200.015.1447385
GCTACCG28050.015.08082270
CGTAGAT59450.015.0493841
CGTGGGT57550.014.44928357
TAGGTCG10800.014.4381573
Produced by FastQC (version 0.11.5)