FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005293634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005293634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15720941
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447810.2848493611164879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA389490.2477523451045329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCGG33600.024.9672222
TAGCATA13250.023.283839
TTAGCCC36250.022.56912630
GATTAGC38200.022.41392928
ACTTTCG40250.020.84220121
GCTACCG36500.020.48862570
GGATTAG43050.020.05014827
CGGATTA43850.019.92227426
CGTAATT6050.019.01677970
TCTCGAT34300.018.62337148
CTCGATT34600.018.46478349
TTCGGAT46250.018.21335824
GGCGCGT22200.018.1082345
TTCTCGA37150.017.56487547
TGGCGCG22700.017.4030444
ATTAGCC49150.017.20897129
ATGTAAG49050.017.11082639
TCGGATT51400.016.38863625
ATCTACT52200.016.33669717
TCTACTT52500.016.30880418
Produced by FastQC (version 0.11.5)